| Project/Area Number |
17590934
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Yamaguchi University |
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Principal Investigator |
EMOTO Masahiro Yamaguchi University, Hospital, research associate, 医学部附属病院, 助手 (50294640)
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| Co-Investigator(Kenkyū-buntansha) |
TSURU Masatoshi Yamaguchi University, Hospital, research associate, 医学部附属病院, 助手 (20379960)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | membrane microdomain / insulin / glucose transporter / actin / molecular motors / GPI anchored proteins / インスリン抵抗性 / TNF-α / 脂肪細胞 / カベオリン |
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| Research Abstract |
We determined the active microdomains for glucose-transport "Hot-spots" in adipocytes. These areas are well actin-reorganized membranes and activated by insulin. We found two independent proteins ; Rmel and GPI anchored protein p69, as regulators of glucose transport activity. Rmel inhibits GLUT4 entry into caveolin rafts; in contrast, p69 promotes it. These data suggests that membrane microdomains are important for glucose homeostasis.
Furthermore, we identified IRS1 foci just beneath the "hot-spots" area, where insulin signaling are inhibited by signal cross-talk with TNF alpha. This micro-structures are also functional for normal regulation for glucose metabolism.
These results suggest that membrane microdomains/ microstructures are functional organization for normal glucose metabolism.
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