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Functional Analysis of the human CTLH complex, Homolog of Yeast GID complex, in Muscle Cells

Research Project

Project/Area Number 17590944
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionYokohama City University

Principal Investigator

UEDA Atsuhisa  Yokohama City University, Hospital, associate Professor, 附属病院, 準教授 (60295483)

Co-Investigator(Kenkyū-buntansha) KANEKO Takeshi  Yokohama City University, Medical Center, Professor, 附属市民総合医療センター, 教授 (90275066)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsGID complex / ARMC8 / p44 CTLH / p48 EMLP / RanBPM / CTLHドメイン / グルコース効果 / プロテオリシス
Research Abstract

Ran binding protein in microtubule organising centre (RanBPM) was originally isolated as a protein that binds to the small GTPase Ran. RanBPM also associates with the HGF receptor MET, p75 the neurotrophin receptor, and integrin LFA-1, and modifies the signaling of some of these molecules. Therefore, RanBPM may function as a scaffolding protein that coordinates the signaling input of cell surface receptors with intracellular signaling pathways. Previously, we identified RanBPM as a component of a 20S large protein complex. Here we purified the complex from soluble extract of HEK293 cells by an antibody against RanBPM, and identified Muskelin, ARMC8α, p48EMLP, ARMC8β, and p44CTLH as complex components by tandem mass spectrometry. ARMC8α and ARMC8βare novel armadillo-repeat proteins. Since the N-terminal 364 amino acids of ARMC8a and ARMC8βwere completely conserved, these proteins are probably alternatively spliced products from the same gene. Interestingly, RanBPM, Muskelin, p48EMLP, and p44CTLH possess LisH/ CTLH motifs, which are present in proteins involved in microtubule dynamics, cell migration, nucleokinesis, and chromosome segregation. Immunoblot analysis showed dominant expression of ARMC8α, ARMC8β, p48EMLP, and p44CTLH mRNAs in skeletal muscle and testis. We next confirmed the in vivo association of each complex component by co-immunoprecipitation assays using the Cos-7 cells in which these components were exogenously overexpressed. Pull-down assay using bacterially-expressed Twa1 revealed that each In vitro-translated component could bind to the Twa1. Finally, we confirmed the cellular co-localization of these proteins. Taken together, we revealed that RanBPM, Muskelin, p48EMLP, Twa1, p44CTLH, and ARMC8 form complexes in cells.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (11 results)

All 2007 2006 2005

All Journal Article (11 results)

  • [Journal Article] RanBPM, Muskelin, p48EMLP, p44CTLH and the armadillo-repeat proteins ARMC8α and ARMC8β are components of the CTLH complex2007

    • Author(s)
      Kobayashi N, Ueda A, et al.
    • Journal Title

      Gene (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Tumor Necrosis Factor α Acceleration of Inflammatory Responses by Down-Regulation Heme Oxygenase 1 in Human Peripheral Monocytes2007

    • Author(s)
      Kirino Y, Ueda A, et al.
    • Journal Title

      Arthriis and Rheumatism 56

      Pages: 464-475

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Regulatory Role of Heme Oxygenase 1 in Inflammation of Rheumatoid Arthritis2006

    • Author(s)
      Kobayashi H, Ueda A, et al.
    • Journal Title

      Arthriis and Rheumatism 54

      Pages: 1132-1142

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transactivation of MCP-1/CCL2 by beta-catenin/TCF-4 in human breast cancer cells.2006

    • Author(s)
      Mestdagt M, Ueda A, et al.
    • Journal Title

      Int J Cancer 1118 (1)

      Pages: 35-42

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transactivation of MCP-1/CCL2 by beta-catenin/TCF-4 in human breast cancer cells.2006

    • Author(s)
      Mestdagt M, Ueda A, et al.
    • Journal Title

      Int.J Cancer 1118

      Pages: 35-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulatory Role of Heme Oxygenase 1 in Inflammation of Rheumatoid Arthritis2006

    • Author(s)
      Kobayashi N, Ueda A, et al.
    • Journal Title

      Arthriis and Rheumatism 54

      Pages: 1132-1142

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Transactivation of MCP-1/CCL2 by beta-catenin/TCF-4 in human breast cancer cells.2006

    • Author(s)
      Mestdagt M, Ueda A, et al.
    • Journal Title

      Int J Cancer 1118(1)

      Pages: 35-42

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Transactivation of MCP-1/CCL2 by beta-catenin/TCF-4 in human breast cancer cells.2006

    • Author(s)
      Mestdagt M, Ueda_A, et al.
    • Journal Title

      Int.J Cancer 1118(1)

      Pages: 35-42

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Heme oxygenase-1 inhibits cigarette smoke-induced increase in the tracheal mucosal permeabilty in guinea pigs in vivo.2005

    • Author(s)
      Tagawa A, Ueda_A, et al.
    • Journal Title

      Inflamm Res, 54(5)

      Pages: 229-234

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Increased serum HO-1 in hemophagocytic syndrome and adult-onset Stills disease : use in the differential diagnosis of hyperferritinemia.2005

    • Author(s)
      Kirino Y.Ueda A et al.
    • Journal Title

      Arthritis Res Ther. 7(3)

      Pages: 616-624

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Adenovirus-mediated transfer and overexpression of heme oxygenase 1 cDNA in lungs attenuates elastase-induced pulmonary emphysema in mice.2005

    • Author(s)
      Shinohara T, Ueda A
    • Journal Title

      Human Gene Ther 16(3)

      Pages: 318-327

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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