• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification and characterization of a novel human thyroid hormone receptor isoform

Research Project

Project/Area Number 17590973
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionNational Hospital Organization, Kyoto Medical Center, Clinical Research Institute

Principal Investigator

TAGAMI Tetsuya  Kyoto Medical Center, Clinical Research Institute, Department of Endocrinology, Head of Laboratory, 内分泌研究部・分子内分泌研究室, 室長 (60273439)

Co-Investigator(Kenkyū-buntansha) MORIYAMA Kenji  Kyoto Medical Center, Clinical Research Institute, Department of Endocrinology, Investigator, 内分泌研究部, 研究員 (00301739)
NARUSE Mitsuhide  Kyoto Medical Center, Clinical Research Institute, Department of Endocrinology, Head of Department, 内分泌研究部, 部長 (40120018)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsThyroid hormone / Nuclear Receptor / Thyroid Hormone Receptor / Cloning / Transcription Factor
Research Abstract

Thyroid hormone exerts a pleiotropic effect on development and homeostasis. A novel thyroid hormone receptor β isoform (hereafter referred to as TRβ4) was cloned using PCR from a human pituitary cDNA library as a template. Analysis of the PCR products revealed a 137-bp insertion, which contains a stop codon in the middle, between the 5^<th> and 6^<th> exons that encode the ligand-binding domain of TRβ. The corresponding sequence of this insertion exists within the 5^<th> intron of the human TRβ gene and consensus splice sequences were found at the junction sites. RACE analysis revealed that TRβ4 is a carboxyl-terminal splicing variant of TRβ1. RT-PCR and. Northern blot analyses indicate that TRβ4 mRNA is expressed in various human tissues, and especially abundant in skeletal muscle. The TRβ4 protein was unable to bind thyroid hormone (T3) and transient transfection assays demonstrate that TRβ4 construct does not mediate regulation of either positively or negatively regulated reporter genes. Furthermore, TRβ4 interacts minimally with RXR and co-repressor proteins. TRβ4 inhibited transcription mediated by functional TRs when co-transfected with TRβ1, TRβ2 or TRα1. This dominant negative activity of TRβ4 was comparable to TRα2, but not as potent as mutant TRs that are found in the patients with resistance to thyroid hormone (RTH). Thus, this novel isoform may modulate T3 action as an endogenous antagonist in the tissue or cellular context.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (15 results)

All 2007 2006 2005 Other

All Journal Article (15 results)

  • [Journal Article] Anti-thyroid Drugs Inhibit Thyroid Hormone Receptor-mediated Transcription.2007

    • Author(s)
      Moriyama K, Tagami T, Usui T, Naruse M, Nambu T, Hataya Y, Kanamoto N, Li Y, Yasoda A, Arai H, Nakao K
    • Journal Title

      J Clin Endocrinol Metab 92・3

      Pages: 1066-1072

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Anti-thyroid Drugs Inhibit Thyroid Hormone Receptor-mediated Transcription.2007

    • Author(s)
      Moriyama K, Tagami T, Usui T, Naruse M, Nambu T, Hataya Y, Kanamoto N, Li Y, Yasoda A, Arai H, Nakao K.
    • Journal Title

      J Clin Endocrinol Metab 92(3)

      Pages: 1066-1072

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 新規TRβアイソフオームのクローニング.2006

    • Author(s)
      田上哲也, 森山賢治, 臼井 健, 長田太助, 島津 章, 成瀬光栄
    • Journal Title

      日本内分泌学会雑誌 82・1

      Pages: 54-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cloning of a novel isoform of human thyroid hormone receptor beta.2006

    • Author(s)
      Tagami T, Moriyama K, Usui T, Nagata D, Shimatsu A, Naruse M.
    • Journal Title

      Nippon Naibunpitsu Gakkai Zasshi 82(1)

      Pages: 54-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 新規TRβアイソフォームのクローニング2006

    • Author(s)
      田上 哲也, 森山 賢治, 臼井 健, 長田 太助, 島津 章, 成瀬 光栄
    • Journal Title

      日本内分泌学会雑誌 82・1

      Pages: 54-54

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification and characterization of a novel isoform of human thyroid hormone receptor beta that does not bind T3.2005

    • Author(s)
      Tagami T, Moriyama K, Usui T, Nagata D, Shimatsu A, Naruse M
    • Journal Title

      The 7th international workshop on thyroid hormone resistance Syllabus

      Pages: 15-15

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-thyroidal drugs, PTU and MMI, an alternative action on the transcriptional activity mediated by the thyroid hormone receptors.2005

    • Author(s)
      Moriyama K, Tagami T, Usui T, Naruse M, Hataya Y, Kanamoto N, Li Y, Arai H, Akamizu T, Nakao K
    • Journal Title

      The 7th international workshop on thyroid hormone resistance Syllabus

      Pages: 53-53

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification and characterization of a novel isoform of human thyroid hormone receptor beta that does not bind T3.2005

    • Author(s)
      Tagami T, Moriyama K, Usui T, Nagata D, Shimatsu A, Naruse M.
    • Journal Title

      The 7th international workshop on thyroid hormone resistance. Syllabus

      Pages: 15-15

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-thyroidal drugs, PTU and MMI, an alternative action on the transcriptional activity mediated by the thyroid hormone receptors.2005

    • Author(s)
      Moriyama K, Tagami T, Usui T, Naruse M, Hataya Y, Kanamoto N, Li Y, Arai H, Akamizu T, Nakao K
    • Journal Title

      The 7th international workshop on thyroid hormone resistance. Syllabus

      Pages: 53-53

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A novel isoform of thyroid hormone receptor beta that does not bind T3.2005

    • Author(s)
      Tagami T, Moriyama K, Usui T, Nagata D, Shimatsu A, Naruse M
    • Journal Title

      The 7th international workshop on thyroid hormone resistance Syllabus

      Pages: 15-15

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Clinical and molecular features of a TSH-secreting pituitary microadenoma.2005

    • Author(s)
      Usui T, Izawa S, Sano T, Tagami T, Nagata D, Shimatsu A, Takahashi JA, Naruse M
    • Journal Title

      Pituitary 8

      Pages: 127-134

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 新規TRβアイソフオームのクローニング.

    • Author(s)
      田上哲也
    • Journal Title

      内分泌・糖尿病科 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cloning of a novel thyroid hormone receptor beta isoform.

    • Author(s)
      Tagami T.
    • Journal Title

      Naibunpitsu" Tonyobyoka (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The incidence of gestational hyperthyroidism and postpartum thyroiditis in the treated patients with Graves' disease

    • Author(s)
      Tagami T, Hagiwara H, Kimura T, Usui T, Shimatsu A, Naruse M
    • Journal Title

      Thyroid (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Molecular mechanism of the inhibitory effect of aldosterone on endothelial nitric oxide synthase activity

    • Author(s)
      Nagata D, Takahashi M, Sawai K, Tagami T, Usui T, Shimatsu A, Hirata Y, Naruse M
    • Journal Title

      Hypertension (In press)

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi