Regulation of hematopoiesis by dendritic cells
| Project/Area Number |
17590978
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Akita University |
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Principal Investigator |
OHTEKI Toshiaki (2006) Akita University, Medicine, Professor, 医学部, 教授 (50233200)
川端 良成 (2005) 秋田大学, 医学部, 助手 (70361227)
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| Co-Investigator(Kenkyū-buntansha) |
SAWADA Kenichi Akita University, Medicine, Professor, 医学部, 教授 (90226069)
樗木 俊聡 秋田大学, 医学部, 教授 (50233200)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | CD34 / Erythroblast / Megakaryocyte / Dendritic cells / CD11c / Interleukin-15 / hemophagocytic syndrome / CD11c / 血球貧食症候群 / 巨核球系前駆細胞 / TNF-α / 貪食 / 免疫寛容 |
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| Research Abstract |
The head investigator has been changed from Y.Kawabata to T.Ohteki at 2007. We conducted a study entitled "Regulation of hematopoiesis by dendritic cells (DC)". We planned this study to clarify the physiological and pathological role of hemophagocytosing DC co-developed with hematopoietic progenitors from human hematopoietic stem/progenitor cells (CD34+ cells) and obtained the following results :
1) The costimulation of thrombopoietin (TPO) and tumor necrosis factor-α (TNF-α) generated megakaryocytic progenitors (MPs) and DC together from human CD34+ cells. CD11c+ DCs were found to engulf damaged MPs by TNF-α and to induce autologous T-cell proliferation. An engulfment of blood cells by CD11c+ DCs was also observed in patients with hemophagocytic syndrome. TNF-α induces hemophagocytic DC in erythroid lineage differentiation, as we reported previously. Thus, this study, for the first time, suggested that DC generated under hematopoietic and inflammatory stimuli are involved in hematopoiesis and the subsequent immune responses (Blood 2006;107:1366).
2) Subsequent study revealed that the nature of hemophagocytic DC is different between megakaryocytic and neutrophilic lineage in the way for stimulating T lymphocytes (ASH Annual Meeting Abstracts, Part 1, 108, Issue 11:488a, 2006, in preparation for submitting).
3) The analysis of DNA profiling between non-hemophagocytosing DC and hemophagocytosing DC has not been completely accomplished and been being continued.
4) The induction mechanism of inflammatory diseases by IL-15 produced from dendritic cells (DCs) was investigated. We showed that IL-15 is requisite for the induction of granuloma formation and endotoxin-shock (J Exp Med 2006;203:2329).
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] 赤芽球癆診療の参照ガイド-PRCAの診療基準と診療の参照ガイド作成のためのワーキンググループ-2006
Author(s)
澤田賢一, 浦部晶夫, 中尾眞二, 別所正美, 唐沢正光, 石田陽治, 小松則夫, 増田道彦, 廣川誠, 茂木睦仁, 小峰光博
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Journal Title
臨床血液 47 (4)
Pages: 316-330
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Successful autologous peripheral blood stem cell transplantation using thiotepa in a patient with systemic sclerosis and cardiac involvement.2006
Author(s)
Komatsuda A, Kawabata Y, Horiuchi T, Motegi M, Ozawa M, Fujishima N, Kume M, Hirokawa M, Wakui H, Yamaguchi A, Sawada K.
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Journal Title
Tohoku J Exp Med. 209(1)
Pages: 61-67
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] 赤芽球癆診療の参照ガイド-PRCAの診療基準と診療の参照ガイド作成のためのワーキンググループ-2006
Author(s)
澤田賢一, 浦部晶夫, 中尾眞二, 別所正美, 唐沢正光, 石田陽治, 小松則夫, 増田道彦, 廣川誠, 茂木睦仁, 小峰光博
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Journal Title
臨床血液 47(4)
Pages: 316-330
Related Report
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[Journal Article] Long-term relapse-free survival and overall survival of patients with acquired primary idiopathic PRCA receiving cyclosporine A. A nationwide cohort study in Japan for the PRCA Collaborative Study Group.
Author(s)
Sawada K, Hirokawa M, Fujishima N, Teramura M, Bessho M, Dan K, Tsurumi H, Nakao S, Urabe A, Omine M, Ozawa K for the PRCA Collaborative Study Group.
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Journal Title
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] (2006) IL-15-dependent cross-talk between cDC and pDC is essential for CpG-induced immune activation.
Author(s)
Kawajima, S., Sato, T., Ishida, K., Tada, H., Tezuka, H., Ohteki, T.
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Journal Title
Nature Immunology (in press)
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