• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Application of nuclear transferred embryonic stem cells for reconstitution of hematopoiesis

Research Project

Project/Area Number 17591020
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionCell Engineering Division, RIKEN BioResource Center (RIKEN BRC)

Principal Investigator

NAKAMURA Yukio  RIKEN Bio Resource center, Cell Engineering Division, Head, 細胞材料開発室, 室長 (60231479)

Co-Investigator(Kenkyū-buntansha) HIROYAMA Takashi  RIKEN Bio Resource center, Cell Engineering Division, Scientist, 細胞材料開発室, 研究員 (50373361)
SUDO Kazuhiro  RIKEN Bio Resource center, Cell Engineering Division, Scientist, 細胞材料開発室, 協力研究員 (10392002)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsEmbryonic stem cell / Nuclear transfer / Hematopoiesis / Bone marrow transplantation / Hematopoietic stem cell
Research Abstract

The establishment of a number of human embryonic stem (ES) cell lines has spurred research into the potential applications of such cells. The use of ES cell-derived cell populations for cell therapy appears to be one of the more promising clinical applications. However, there are a few reasons to limit clinical uses of the cells derived from ES cells. One is in the case of a mismatch of major histo-compatibility (MHC) antigen between the ES cells and the recipient's cells. This limitation may be overcome in several ways: an induction of tolerance in the recipient using ES cell-derived antigen-presenting cells; production of many ES cell lines to cover many types of MHC; production of cloned ES cell lines that possess nuclei derived from the recipient, i.e. so called "therapeutic cloning".
The establishment of nuclear transferred ES (ntES) cell line is possible with mouse oocytes and nuclei. On the other hand, although the establishment of human ntES cell line has not been succeeded yet, … More it is highly likely that the technology to establish human ntES cell line will be developed in the future. Prior to the use of human ntES cell lines in the clinic, many basic researches should be performed using mouse and/or primate ntES cell lines.
Since many groups have already reported that hematopoietic cells can be derived from human ES cells, hematopoietic cells may be also derived from human ntES cells. Then, we have tested whether hematopoietic cells can be derived from mouse ntES cells. First, we have analyzed the characteristics of several mouse ntES cell lines. All mouse ntES cell lines tested have demonstrated that they have the characteristics quite similar to ordinary ES cell lines, e.g., in morphology, cell surface phenotype, and gene expression pattern. Next, we have tested whether mouse ntES cells could produce hematopoietic cells following the induction of those cells into hematopoietic cells. As the results, all mouse ntES cell lines tested could produce hematopoietic cells robustly.
We are currently studying whether hematopoietic stem cells are included in the hematopoietic cell population produced from mouse ntES cells. In addition, we will test the potential of those hematopoietic stem cells whether they can reconstitute hematopoietic cells in vivo by transplantation assay. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (12 results)

All 2006 2005 Other

All Journal Article (12 results)

  • [Journal Article] Essential role for gene profiling analysis in the authentication of human cell lines.2006

    • Author(s)
      Yoshino, K., Iimura, E., Saijo, K., Iwase, S., Fukami, K., Ohno, T., Obata, Y., Nakamura, Y.
    • Journal Title

      Human Cell 19

      Pages: 43-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Refinement of cytokine use in the in vitro expansion of erythroid cells.2006

    • Author(s)
      Miharada, K., Hiroyama, T., Sudo, K., Nagasawa, T., Nakamura, Y.
    • Journal Title

      Human Cell 19

      Pages: 30-37

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Long lasting in vitro hematopoiesis derived from primate embryonic stem cells.2006

    • Author(s)
      Hiroyama, T., Miharada, K., Aoki, N., Fujioka, T., Sudo, K., Danjo, I., Nagasawa, T., Nakamura, Y.
    • Journal Title

      Experimental Hematology 34

      Pages: 760-769

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells.2006

    • Author(s)
      Miharada, K., Hiroyama, T., Sudo, K., Nagasawa, T., Nakamura, Y.
    • Journal Title

      Nature Biotechnology 24

      Pages: 1255-1256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] A method for the selection of human embryonic stem cell sub-lines with high replating efficiency after single cell dissociation.2006

    • Author(s)
      Hasegawa, K., Fujioka, T., Nakamura, Y., Nakatsuji, N., Suemori, H.
    • Journal Title

      Stem Cells 24

      Pages: 2649-2660

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Long lasting in vitro hematopoiesis derived from primate embryonic stem cells.2006

    • Author(s)
      Hiroyama, T., Miharada, K., Aoki, N., Fujioka, T., Sudo, K., Danjo, I., Nagasawa, T., Nakamura, Y.
    • Journal Title

      Exp. Hematol. 34

      Pages: 760-769

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells.2006

    • Author(s)
      Miharada, K., Hiroyama, T., Sudo, K., Nagasawa, T., Nakamura, Y.
    • Journal Title

      Nat. Biotechnol. 24

      Pages: 1255-1256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.2006

    • Author(s)
      Sudo, K., Kanno, M., Miharada, K., Ogawa, S., Hiroyama, T., Saijo, K., Nakamura, Y.
    • Journal Title

      Stem Cells (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Lipocalin 2 functions as a negative regulator of red blood cell production in an autocrine fashion.2005

    • Author(s)
      Miharada, K., Hiroyama, T., Sudo, K., Nagasawa, T., Nakamura, Y.
    • Journal Title

      FASEB J. 19

      Pages: 1881-1883

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.

    • Author(s)
      Sudo, K., Kanno, M., Miharada, K., Ogawa, S., Hiroyama, T., Saijo, K., Nakamura, Y.
    • Journal Title

      Stem Cells (doi : 10.1634/stemcells.2006-0504) (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Essential role for gene profiling analysis in the authentication of human cell lines.

    • Author(s)
      Yoshino, K., Iimua, E., Saijo, K., Iwase, S., Fukami, K., Ohno, T., Obata, Y., Nakamura, Y.
    • Journal Title

      Human Cell (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Refinement of cytokine use in the in vitro expansion of erythroid cells.

    • Author(s)
      Miharada, K., Hiroyama, T., Sudo, K., Nagasawa, T., Nakamura, Y.
    • Journal Title

      Human Cell (In press)

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi