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Analysis of multi-step processes of leukemia-genesis in childhood leukemia.

Research Project

Project/Area Number 17591024
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionAichi Cancer Center Research Institute

Principal Investigator

TSUZUKI Shinobu  Aichi Cancer Center, Division of Molecular Medicine, Laboratory head, 遺伝子医療研究部, 室長 (00342965)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsTEL-AML1 / childhood leukemia / array-based CGH / isoform / ETV6-RUNX1 / array-CGH / genomic imbalances / second hits / leukemogenesis / mouse model / retrovirus
Research Abstract

(1)TEL-AML1 fusion gene, generated by t(12 ; 21) translocation, is the commonest abnormality in childhood leukemia, and is exclusively associated with B cell leukemia. We have addressed the mechanism whereby the translocation generates the leukemia, using mouse model. Although we are able to see the fusion is able to block B cell differentiation, and expand cell population, mice did not develop leukemia. We therefore next searched for additional genetic abnormalities which could be linked leukemia-genesis. To this end, we analyzed clinical leukemia samples and cell lines by the use of array-CGH method. We then found that all the cases had at least 2 genetic abnormalities, in addition to TEL-AML1 translocation. Among these, loss of TEL on the untranslocated allele was most commonly found, followed by losses of genes involved in cell cycle regulation, such as BTG1, p16INK4a/ARF. Enforced expressions of the gene products found lost in Reh, a TEL-AML1 cell line, inhibited the cell proliferation. These findings suggest the losses of the genes may partly account for the leukemia-genesis.
(2)Evidence is accumulating that a specific isoform of a given transcription factor is associated with leukemia. There are two isoforms in Runx1/AML1 transcription factor, but their differential roles and possible association with leukemia-genesis have not been well explored. We found that the short isoform, AML1a, is expressed exclusively in CD34-positive immature stem/progenitors among human cord blood cells. We therefore next transduced mouse/human hematopoietic cells using retrovirus/lentivirus to see if the expression of AML1a has any impact on stem/progenitor activities both in vitro and in vivo. We then found that AML1a expression confers cells self-renewal activities, growth advantage, higher engraftment potential. We concluded that the blood cell expansion thus achieved is more likely to obtain additional genetic hits, and thus leading to leukemia.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (16 results)

All 2007 2006 2005 Other

All Journal Article (16 results)

  • [Journal Article] Genetic abnormalities involved in t(12;21) TEL-AMLl acute lymphoblastic leukemia : Analysis by means of array-based comparative genomic hybridization2007

    • Author(s)
      Tsuzuki S, Karnan S, Horibe K, Matsumoto K, Kato K, Inukai T, Goi K, Sugita K, Nakazawa S, Kasugai Y, Ueda R, Seto M
    • Journal Title

      Cancer Science

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic abnormalities involved in t(12;2l) TEL-AML1 acute lymphoblastic leukemia : Analysis by means of array-based comparative genomic hybridization2007

    • Author(s)
      Tsuzuki S, Karnan S, Horibe K, Matsumoto K, Kato K, Inukai T, Goi K, Sugita K, Nakazawa S, Kasugai Y, Ueda R, Seto M
    • Journal Title

      Cancer Science (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isoform-specific potentiation of Stem and Progenitor Cell Engraftment by AML1/RUNX12007

    • Author(s)
      Tsuzuki S, Hong D, Rajeev G, Matsuo K, Seto M, Enver T
    • Journal Title

      Plos Medicine (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic abnormalities involved in t(12;21) TEL-AML1 acute lymphoblastic leukemia : Analysis by means of array-based comparative genomic hybridization2007

    • Author(s)
      Tsuzuki S
    • Journal Title

      Cancer Science (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Isoform-specific potentiation of Stem and Progenitor Cell Engraftment by AML1/RUNX12007

    • Author(s)
      Tsuzuki S
    • Journal Title

      Plos Medicine (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genomewide array-based comparative genomic hybridization analysis of acute promyelocytic leukemia.2006

    • Author(s)
      Karnan S, Tsuzuki S, Kiyoi H, Tagawa H, Ueda R, Seto M, Naoe T
    • Journal Title

      Genes Chromosomes Cancer. 45

      Pages: 420-425

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Genomewide array-based comparative genomic hybridization analysis of acute promyelocytic leukemia.2006

    • Author(s)
      Karnan S
    • Journal Title

      Genes Chromosomes Cancer. 45

      Pages: 420-425

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Developmental impact of leukemic fusion genes on stem cell fate.2005

    • Author(s)
      Eriver T, Tsuzuki S, Greaves M
    • Journal Title

      Ann N Y Acad Sci. 1044

      Pages: 16-23

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] MALT1 contains nuclear export signals and regulates cytoplasmic localization of BCL1O.2005

    • Author(s)
      Nakagawa M, Hosokawa Y, Yonezumi M, Izumiyama K, Suzuki R, Tsuzuki S, Asaka M, Seto M
    • Journal Title

      Blood 106

      Pages: 4210-4216

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparison of genome profiles for identification of distinct subgroups of diffuse large B-cell lymphoma.2005

    • Author(s)
      Tagawa H, Suguro M, Tsuzuki S, Matsuo K, Karnan S, Oshima K, Okamoto M, Morishima Y, Nakamura S, Seto M
    • Journal Title

      Blood 106

      Pages: 1770-1777

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Developmental impact of leukemia fusion genes on stem cell fate.2005

    • Author(s)
      Enver T, Tsuzuki S, Greaves M
    • Journal Title

      Ann N Y Acad Sci. 1044

      Pages: 16-23

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] MALT1 contains nuclear export signals and regulates cytoplasmic localization of BCL10.2005

    • Author(s)
      Nakagawa M, Hosokawa Y, Yonezumi M, Izumiyama K, Suzuki R, Tsuzuki S, Asaka M, Seto M
    • Journal Title

      Blood 106

      Pages: 4210-4216

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] MALT1 contains nuclear export signals and regulates cytoplasmic localization of BCL10.2005

    • Author(s)
      Nakagawa M, Hosokawa Y, Yonezumi M, Izumiyama K, Suzuki R, Tsuzuki S, Asaka M, Seto M.
    • Journal Title

      Blood. 106

      Pages: 4210-4216

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Developmental impact of leukemic fusion genes on stem cell fate.2005

    • Author(s)
      Enver T, Tsuzuki S, Brown J, Hong D, Gupta R, Ford T, Egucchi MI, Egucchi M, Greaves M.
    • Journal Title

      Ann N Y Acad Sci. 1044

      Pages: 16-23

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Comparison of genome profiles for identification of distinct subgroups of diffuse large B-cell lymphoma.2005

    • Author(s)
      Tagawa H, Suguro M, Tsuzuki S, Matsuo K, Karnan S, Ohshima K, Okamoto M, Morishima Y, Nakamura S, Seto M.
    • Journal Title

      Blood. 106

      Pages: 1770-1777

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isoform-specific potentiation of Stem and Progenitor Cell Engraftment by AML1/RUNX1

    • Author(s)
      Tsuzuki S, Hong D, Rajeev G, Matsuo K, Seto M, Enver T
    • Journal Title

      Plos Medicine (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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