The role of retroelement in rheumatoid arthritis
Grant-in-Aid for Scientific Research (C)
|Allocation Type||Single-year Grants |
|Research Institution||National Hospital Organization Osaka Minami Medical Center Department of Clinical Research |
OHSHIMA Shiro National Hospital Organization Osaka Minami Medical Center, Department of Clinical Research, The head of Autoimmune disease lab, 臨床研究部・免疫異常疾患研究室長 (50362728)
SAEKI Yukihiko National Hospital Organization Osaka Minami Medical Center, Department of Clinical Research, Director, 臨床研究部, 部長 (40240840)
|Project Period (FY)
2005 – 2006
Completed (Fiscal Year 2006)
|Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
|Keywords||retroelement / galectin3|
We analyzed the expression of all known retroelements using DNA chip developed by Dr.W Seifarth. We found some expression patterns of retroelements, as shown below.
1)Some Retroelements including HERV-K mRNA are expressed even in normal samples.
HML-4 (HERV-K) Seq10、K-T47D
2)There are no RA-specific expression of Retroelements
3)There are cell-type specific expression patterns for some HERVs (ST, SF and MNC).
HML-6 (HERV-K) Seq56
HML-10 (HERV-K (C4)) K-C4
Mammalian ERV MoMLV、OvEV-2
HML-2 (HERV-K) K10、U87Zir
HML-3 (HERV-K) SLE666、Seq34、Seq42
HML-3 (HERV-K) Seq43
4)The expressions of some retroelements were reduced in RA synovial tissues.
HML-6 (HERV-K) HML-6、Seq38
HML-3 (HERV-K) RT244、Seq26
HML-nd K (U39937)
The investigation of downstream of L1 retrotransposon showed Galectin3 (Gal-3) and Galectin binding protein (Gal-3 BP) were enhanced in local rheumatoid synovium. Moreover, Gal-3 can induce MMP-1,-3 productions in dose dependent manner. Gal-3 plays the important role in induction of MMPs through IL-1β and TNFα independent pathway. Taken together, Gal-3 might contribute to the bone/cartilage destruction by induction of MMPs in RA.
Report (3 results)
Research Products (4 results)