Expression and functional analysis of cellular anti-HIV factor among HIV-infected Long-Term Nonprogressor

• Project/Area Number: 17591062
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Osaka National Hospital, National Health Organization
• Principal Investigator: YAMAMOTO Yoshihiko Osaka National Hospital, National Health Organization, Investigator, 研究員 (30393292)
• Co-Investigator(Kenkyū-buntansha): SHIRASAKA Takuma Osaka National Hospital, National Health Organization, 部長 (40359310) ; UCHIYAMA Tsuneo Graduate School of Tokushima University, Associate Professor, 大学院, 助教授 (90151901) ; GATANAGA Hiroyuki International Medical Center of Japan, AIDS Clinical Center, 厚生労働技官
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2005: ¥3,100,000 (Direct Cost: ¥3,100,000)
• Keywords: HIV / Long Term Nonprogressors (LTNP) / AOBEC3G / vif

Research Abstract

1,Search of HIV-infected Long Term Nonprogressors
We searched the patients who maintained CD4+-T Cell count more than 500 for recent six years among more than 2100 patients who hospitalized to Osaka National hospital and International Medical Center of Japan. As a result, 15 patients were found.
2,Expression of APOBEC3G
We received anti-APOBEC3G antibody and it successfully showed the high expression of intracellular APOBEC3G through the FI assay. However, this expression level in PBMC goes up and down in each lot. Thus, we constructed the standard cell line which expresses the APOBEC3G protein constantly, and this cell line was useful for the normalization of APOBEC3G. Generally, non-Adherent cells were used for the HIV infection assay. However, it is difficult to make and maintain the appropriate APOBEC3G expression constantly. Then, we establish the cell line which is based on the adherent cell line, 293T cells. Among the clone A3G-C1 and A3G-C4, vif-defected HIV could not infect with. APOBEC3G expression in these cells was high and functional. By these cell line, quantification of APOBEC3G is possible and the measurement of APOBEC3G expression among Long Term Nonprogressors and slow progressors.

Research Products

• [Journal Article] Generation and characterization of APOBEC3G-positive 293T cells for HIV-1 Vif study. (2007)
  • Author(s): Piroozmand A, Yamamoto Y, Khamsri B, Fujita M, Uchiyama T, Adachi A
  • Journal Title: Journal of Medical Investigation 54・1-2 Pages: 154-158
  • NAID: 120006470126
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Convalescence of atypical reversible posterior leukoencephalopathy syndrome in human immunodeficiency virus infection. (2007)
  • Author(s): Tanioka R, Yamamoto Y, Sakai M, Makie T, Mori M, Uehira T, Shirasaka T
  • Journal Title: Journal of Medical Investigation 54・1-2 Pages: 191-194
  • NAID: 120006470132
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Tuberculous and syphilitic meningitis in a patient infected with the human immunodeficiency virus (2007)
  • Author(s): Makie T, Yamamoto Y, Uehira T, Shirasaka T, Takeda M
  • Journal Title: Internal Medicine 46・7 Pages: 415-418
  • NAID: 130000078773
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Generation and characterization of APOBEC3G-positive 293T cells for HIV-1 Vif study. (2007)
  • Author(s): Piroozmand A, Yamamoto Y, Khamsri B, Fujita M Uchiyama T, Adachi A.
  • Journal Title: J Med Invest. 54(1-2) Pages: 154-158
  • NAID: 120006470126
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Convalescence of atypical reversible posterior leukoencephalopathy syndrome in human immunodeficiency virus infection. (2007)
  • Author(s): Tanioka R, Yamamoto Y, Sakai M, Makie T, Mori M, Uehira T, Shirasaka T.
  • Journal Title: J Med Invest. 54(1-2) Pages: 191-194
  • NAID: 120006470132
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Tuberculous and syphilitic meningitis in a patient infected with the human immunodeficiency virus. (2007)
  • Author(s): Makie T, Yamamoto Y, Uehira T, Shirasaka T, Takeda M.
  • Journal Title: Intern Med.(Epub 2007 Apr 2.) 46(7) Pages: 415-418
  • NAID: 130000078773
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Drug-resistant HIV-1 prevalence in patients newly diagnosed with HIV/AIDS in Japan. (2007)
  • Author(s): Gatanaga H, Ibe S, Matsuda M, Yoshida S, Asagi T, Kondo M, Sadamasu K, Tsukada H, Masakane A, Moti H, Takata N, Minami R, Tateyama M, Koike T, Itoh T, Imai M, Nagashima M, Gejyo F, Ueda M, Hamaguchi M, Kojima Y, Shirasaka T, Kimura A, Yamamoto M, Fujita J, Oka S, Sugiura W.
  • Journal Title: Antiviral Research (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] HLA-Cw8 primarily associated with hypersensitivity to nevirapine. (2007)
  • Author(s): Gatanaga H, Yazaki H, Yanuma J, Honda M, Genka I, Teruya K, Tachikawa N, Kikuchi Y, Oka S.
  • Journal Title: AIDS Vol.21 Pages: 264-265
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Generation and characterization of APOBEC3G-positive 293T cells for HIV-1 Vif study. (2007)
  • Author(s): Piroozmand A, Yamamoto Y, Khamsri B, Fujita M, Uchiyama T, Adachi A.
  • Journal Title: Journal of Medical Investigation 54・1-2 Pages: 154-8
  • NAID: 120006470126
• [Journal Article] Convalescence of atypical revesible posterior leukoencephalopathy syndrome in human immunodeficiency virus infection. (2007)
  • Author(s): Tanioka R, Yamamoto Y, Sakai M, Makie T, Mori M, Uehira T, Shirasaka T.
  • Journal Title: Journal of Medical Investigation 54・1-2 Pages: 191-4
• [Journal Article] Tuberculous and syphilitic meningitis in a patient infected with the human immunodeficiency virus (2007)
  • Author(s): Makie T, Yamamoto Y, Uehira Y, Shirasaka T, Takeda M.
  • Journal Title: Internal Medicine 46・7 Pages: 415-8
  • NAID: 130000078773
• [Journal Article] Generation of HIV-1 derivatives that productively infect macaque monkey lymphoid cells. (2006)
  • Author(s): Kamada K, Igarashi T, Martin MA, Khamsri B, Hatcho K, Yamashita T, Fujita M, Uchiyama T, Adachi A
  • Journal Title: Proceedings National Academy of Science USA 103・45 Pages: 16959-16964
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effects of lysine to arginine mutations in HIV-1 Vif on its expression and viral infectivity. (2006)
  • Author(s): Khamsri B, Fujita M, Kamada K, Piroozmand A, Yamashita T, Uchiyama T, Adachi A
  • Journal Title: International Journal of Molecular Medicine 18・4 Pages: 679-683
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mutations other than 103N in human immunodeficiency virus type 1 reverse transcriptase (RT) emerge from K103R polymorphism under non-nucleoside RT inhibitor pressure (2006)
  • Author(s): Gatanaga H, Hachiya A, Kimura S, Oka S
  • Journal Title: Virology 344 Pages: 354-362
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] S-nitrosoglutathione modulates CXCR4 and ICOS expression. (2006)
  • Author(s): Yamamoto Y, Pahwa R, Pahwa S.
  • Journal Title: Cell Mol Biol Lett. 11(1) Pages: 30-36
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Generation of HIV-1 derivatives that productively infect macaque monkey lymphoid cells. (2006)
  • Author(s): Kamada K, Igarashi T, Martin MA, Khamsri B, Hatcho K, Yamashita T, Fujita M, Uchiyama T, Adachi A.
  • Journal Title: Proc Natl Acad Sci USA. 103(45) Pages: 16959-16964
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of lysine to arginine mutations in HIV-1 Vif on its expression and viral infectivity. (2006)
  • Author(s): Khamsri B, Fujita M, Kamada K, Piroozmand A, Yamashita T, Uchiyama T, Adachi A.
  • Journal Title: Int J Mol Med. 18(4) Pages: 679-683
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Morphological study on biologically distinct vpx/vpr mutants of HIV-2. (2006)
  • Author(s): Piroozmand A, Khamsri B, Fujita M, Adachi A, Uchiyama T.
  • Journal Title: J Med Invest. 53(3-4) Pages: 271-276
  • NAID: 130004465103
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Construction of gag-chimeric viruses between HIV-1 and SIVmac that are capable of productive multi-cycle infection. (2006)
  • Author(s): Kamada K, Yoshida A, Khamsri B, Piroozm, A, Yamashita T, Uchiyama T, Fujita M, Adachi A.
  • Journal Title: Microbes Infect. 8(4) Pages: 1075-1081
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparative study on the structure and cytopathogenic activity of HIV Vpr/Vpx proteins. (2006)
  • Author(s): Khamsri B, Murao F, Yoshida A, Sakurai A, Uchiyama T, Shirai H, Matsuo Y, Fujita M, Adachi A.
  • Journal Title: Microbes Infect.(Epub 2005 Aug 8.) 8(1) Pages: 10-15
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Difference of progression to AIDS according to CD4 cell count, plasma HIV RNA level and the use of antiretroviral therapy amon : HIV patients infected through blood products in Japan. (2006)
  • Author(s): Kawado M, Hashimoto S, Yamaguchi T, Oka S, Yoshizaki K, Kimura S, Fukutake K, Higasa S, Shirasaka T.
  • Journal Title: J Epidemiol 16(3) Pages: 101-106
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mutations other than 103N in human immunodeficiency virus type 1 reverse transcriptase (RT) emerge from K103R polymorphism under non-nucleoside RT inhibitor pressure. (2006)
  • Author(s): Gatanaga H, Hachiya A, Kimura S, Oka S.
  • Journal Title: Tirology Vol.344 Pages: 354-362
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Altered HIV-1 Gag protein interactions with cyclophilin A (CypA) upon the acquisition of H219Q and H219P substitutions in the CypA binding loop. (2006)
  • Author(s): Gatanaga H, Das D, Suzuki Y, Yeh DD, Hussain KA, Ghosh AK, Mitsuya H.
  • Journal Title: Tournal of Biological Chemistry Vol.281 Pages: 1241-1250
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cooperative contribution of Gag substitutions to nelfinavir-dependent enhancement of precursor cleavage and replication of human immunodeficiency virus type-1. (2006)
  • Author(s): Matsuoka-Aizawa S, Gatanaga H, Sato H, Koike K, Kimura S, Oka S.
  • Journal Title: kntiviral Research Vol.70 Pages: 51-59
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Urinary 2-microglobulin as a possible sensitive marker for renal injury caused by tenofovir clisproxil fumarate. (2006)
  • Author(s): Gatanaga H, Tachikawa N, Kikuchi Y, Teruya K, Genka I, Honda M, Tanuma J, Yazaki H, Ueda A, Kimura S, Oka S.
  • Journal Title: SIDS Research and Human Retroviruses Vol.22 Pages: 744-748
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure-based design of novel HIV-1 protease inhibitors to combat drug resistance. (2006)
  • Author(s): Ghosh AK, Sridhar PR, Leshchenko S, Hussain AK, Li J, Kovalevsky AY, Walters DE, Wedekind JE, Grum-Tokars V, Das D, Koh Y, Maeda K, Gatanaga H, Weber IT, Mitsuya H.
  • Journal Title: Journal of Medical Chemistry Vol.49 Pages: 5252-5261
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Distinct expression of Kaposi's sarcoma-associated herpesvirus-encoded proteins in Kaposi's sarcoma and multicentric Castleman's disease. (2006)
  • Author(s): Abe Y, Matsubara D, Gatanaga H, Oka S, Kimura S, Sasao Y, Saitoh K, Fujii T, Sato Y, Sata T, Katano H.
  • Journal Title: Pathology International Vol.56 Pages: 617-624
  • NAID: 10019268757
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Generation of HIV-1 derivatives that productively infect macaque monkey lymphoid cells. (2006)
  • Author(s): Kamada K, Igarashi T, Martin MA, Khasri B, Hatcho K, Yamashita T, Fujita M, Uchiyama T, Adachi A.
  • Journal Title: Proceedings National Academy of Science U S A 103・45
• [Journal Article] Effects of lysine to arginine mutations in HIV-1 Vif on its expression and viral infectivity. (2006)
  • Author(s): Khamsri B, Fujita M, Kamada K, Piroozmand A, Yamashita T, Uchiyama T, Adachi A.
  • Journal Title: International Journal of Molecular Medicine 18・4 Pages: 679-83
• [Journal Article] S-Nitrosoglutathione modulates CXCR4 and ICOS expression (2006)
  • Author(s): Yamamoto Y, Pahwa R, Pahwa S
  • Journal Title: Cellular and Molecular Biology Letters 11・1 Pages: 33-39
• [Journal Article] Altered HIV-1 Gag protein interactions with cyclophilin A (CypA) upon the acquisition of H219Q and H219P substitutions in the CypA binding loop. (2006)
  • Author(s): Gatanaga H, Das D, Suzuki Y, Yeh DD, Hussain KA, Ghosh AK, Mitsuya H.
  • Journal Title: Journal of Biological Chemistry 281 Pages: 1241-1250
• [Journal Article] Mutations other than 103N in human immunodeficiency virus type 1 reverse transcriptase (RT) emerge from K103R polymorphism under non-nucleoside RT inhibitor pressure. (2006)
  • Author(s): Gatanaga H, Hachiya A, Kimura S, Oka S
  • Journal Title: Virology 344 Pages: 354-362
• [Journal Article] Establishment of a biological assay system for human retroviral protease activity. (2005)
  • Author(s): Yoshida A, Piroozm, A, Sakurai A, Fujita M, Uchiyama T, Kimura T, Hayashi Y, Kiso Y, Adachi A.
  • Journal Title: Microbes Infect. 7(5-6) Pages: 820-824
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Unique characteristics of HIV-1 Vif expression. (2005)
  • Author(s): Wang H, Sakurai A, Khamsri B, Uchiyama T, Gu H, Nakata A, Kamiguchi H
  • Journal Title: Microbes Infect. 7(3) Pages: 385-390
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Spontaneous recovery of hemoglobin and neutrophil levels in Japanese patients on a long-term CombivirR containing regimen. (2005)
  • Author(s): Matushita S, Yoshimura K, Kimura T, Uehira T, Takano M, ETO K, Shirasaka T, Mituya H, Oka S
  • Journal Title: J.Clin.Virol. 33(3) Pages: 188-193
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Relatonship of CD4+CD25+ regulatory T cells to immune status in HIV-infected patients. (2005)
  • Author(s): Tunemi S, Iwasaki T, Imado T, Higasa S, Kakishita E, Shirasaka T, Sano H.
  • Journal Title: AIDS 19(9) Pages: 879-886
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] わが国におけるAIDSへの対応-もっと行動を- (2005)
  • Author(s): 白阪琢磨
  • Journal Title: 医療 59・12 Pages: 635-636
  • NAID: 130004107807
• [Journal Article] Unique characteristics of HIV-I Vif expression. (2005)
  • Author(s): Wang, H., Sakurai, A., Khamsri, B., Uchiyama, T., Gu, H., Adachi, A., Fujita, M.
  • Journal Title: Microbes and Infection 7・3 Pages: 385-390
• [Journal Article] Growth of typhus group and spotted fever group rickettsiae in insect cells. (2005)
  • Author(s): Uchiyama, T.
  • Journal Title: Annals of the New York Academy of Sciences 1063 Pages: 215-221
• [Journal Article] Determination of HIV-1 infectivity by lymphocytic cell lines with integrated luciferase gene. (2004)
  • Author(s): Nagao T, Yoshida A, Sakurai A, Piroozmand A, Jere A, Fujita M, Uchiyama T, Adachi A.
  • Journal Title: Int J Mol Med. 14(6) Pages: 1073-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2007. A protein from a parasitic microorganism, Rickettsia prowazekii, can cleave the signal sequences of proteins targeting mitochondria.
  • Author(s): Kitada, S., Uchiyama, T., Funatsu, T., Kitada, Y., Ogishima, T., Ito, A.
  • Journal Title: Journal of Bacteriology 189 Pages: 844-850
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2006. Murine typhus from Vietnam, imported into Japan.
  • Author(s): Azuma, M., Nishioka, Y., Ogawa, M., Takasaki, T., Sone, S., Uchiyama, T.
  • Journal Title: Emerging Infectious Diseases. 12 Pages: 1466-1468
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 2005. Growth of typhus group and spotted fever group rickettsiae in insect cells.
  • Author(s): Uchiyama, T.
  • Journal Title: Annals of the New York Academy of Sciences 1063 Pages: 215-221
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] HIV Q＆A(改訂第2版)免疫再構築症候群とは何か (2006)
  • Author(s): 山本善彦
  • Total Pages: 3
  • Publisher: 医学ジャーナル社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] HIV Q＆A (改訂第2版) 免疫再構築症候群とは何か (2006)
  • Author(s): 山本善彦
  • Total Pages: 3
  • Publisher: 医学ジャーナル社