Humanized model mouse based on the structures of proteins

• Project/Area Number: 17591081
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Gifu University
• Principal Investigator: YAMAMOTO Yutaka Gifu University, Graduate School of Medicine, Part-time Lecturer (50397329)
• Co-Investigator(Kenkyū-buntansha): KATO Zenichiro Gifu University, Hospital, Lecturer (90303502) ; KONDO Naomi Gifu University, Graduate School of Medicine, Professor (50124714)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: IL-18 / RECEPTOR / MODEL

Research Abstract

The research was performed based on the three dimensional structures of the relevant proteins. The structures were analyzed by multi dimensional, multi nuclear, nuclear magnetic resonance techniques. and crystallography or in silico simulation and modeling. To obtain this information, we established the system to analyze the structures developing new techniques. The obtained structures were used for analysis of the receptor activation mechanisms or the intracellular signaling process. The biochemical and physical data obtained here can be used for the structure based drug design. Through this research, we could make a clue to develop the new drugs based on the structure.

Research Products

• [Journal Article] A novel polymorphism in the 5-lipoxygenase gene associated with bronchial asthma in Japanese children (2008)
  • Author(s): Bai CY, Matsui E, Ohnishi H, Kimata K, Kasahara K, Kaneko H, Kato Z, Fukao T, Kondo N.
  • Journal Title: Int J Mol Med 21 Pages: 139-144
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The response of bovine beta-lactoglobulin-specific T-cell clones to single amino acid substitutions of T-cell core epitope. (2008)
  • Author(s): Kondo M, Kaneko H, Fukao T, Suzuki K, Sakaguchi H, Shinoda S, Kato Z, Matsui E, Teramoto T, Nakano T, Kondo N.
  • Journal Title: Pediatric Allergy and Immunology (Epub ahead)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A novel polymorphism in the 5-lipoxygenase gene associated with bronchial asthma in japanese children (2008)
  • Author(s): Bai, CY., Matsui, E., Ohnishi, H., Kimata, K., Kasahara, K., Kaneko, H., Kato, Z., Fukao, T., Kondo, N
  • Journal Title: Int J Mol Med 21 Pages: 139-144
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The response of bovine beta-lactoglobulin-specific T-cell clones to single amino acid substitutions of T-cell core epitope (2008)
  • Author(s): Kondo, M., Kaneko, H., Fukao, T., Suzuki, K., Sakaguchi, H., Shinoda, S., Kato, Z., Matsui, E., Teramoto, T., Nakano, T., Kondo, N
  • Journal Title: Pediatric Allergy and Immunology
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel polymorphism in the 5-lipoxygenase gene associated with bronchial asthm a in japanese children (2008)
  • Author(s): Bai CY, Matsui E, Ohnishi H, Kimata K, Kasahara K, Kaneko H, Kato Z, Fukao T, Kondo N.
  • Journal Title: Int J Mol Med 21 Pages: 139-144
  • Peer Reviewed
• [Journal Article] Lymphocyte responses to chymotrypsin- or trypsin V-digested β-lactoglobulin in patients with cow's milk allergy. (2007)
  • Author(s): Kondo M, Fukao T, Shinoda S, Kawamoto N, Kaneko H, Kato Z, Matsui E, Teramoto T, Nakano T, Kondo N: Allergy,
  • Journal Title: Asthma and Clinical Immunology 3 Pages: 1-9
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Lymphocyte responses to chymotrypsin- or trypsin V-digested β-lactoglobulin in patients with cow's milk allergy (2007)
  • Author(s): Kondo, M., Fukao, T., Shinoda, S., Kawamoto, N., Kaneko, H., Kato, Z., Matsui, E., Teramoto, T., Nakano, T., Kondo, N
  • Journal Title: Allergy,Asthina and Clinical Immunology 3 Pages: 1-9
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Lymphocyte responses to chymotrypsin-or trypsin V-digested β-lactoglobulin in patients with cow's milk allergy. (2007)
  • Author(s): Kondo M, Fukao T, Shinoda S, Kawamoto N, Kaneko H, Kato Z, Matsui E, Teramoto T, Nakano T, Kondo N: Allergy,
  • Journal Title: Asthma and Clinical Immunology 3 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Development of fluorescence-linked immunosorbent assay for high throughput screening of interferon-gamma. (2006)
  • Author(s): matsukuma E, Kato Z, Omoya K, Hashimoto K, Li A, Yamamoto Y, Ohnishi H, Hiranuma H, Komine H, Kondo N.
  • Journal Title: Allergol Intternational 55 Pages: 49-54
  • NAID: 130004476881
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Development of fluorescence-linked immunosorbent assay for high throughput screening of interferon-gamma (2006)
  • Author(s): Matsukuma, E., Kate, Z. Omoya, K., Hashimoto, K., Li, A., Yamamoto, V., Ohnishi, H., Hiranuma, H., Komine, H., Kondo, N
  • Journal Title: Allergol Int 55(1) Pages: 49-54
  • NAID: 130004476881
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of fluorescence-linked immunosorbent assay for high throughput screening of interferon-gamma. (2006)
  • Author(s): Matsukuma E, Kato Z, Omoya K, Hashimoto K, Li A, Yamamoto Y, Ohnishi H, Hiranuma H, Komine H, Kondo N
  • Journal Title: Allergol International 55 Pages: 49-54
  • NAID: 130004476881
• [Journal Article] Development of Fluorescence-linked Immunosorbent Assay for High Throughput Screening of Interferon-γ (2006)
  • Author(s): Matsukuma E, Kato Z, Yamamoto Y, Kondo N, et al.
  • Journal Title: Allergology International 55 Pages: 49-54
  • NAID: 10020557520
• [Journal Article] New Methods for Clinical Proteomics in Allergy (2005)
  • Author(s): Kato Z and Kondo N
  • Journal Title: Allergology International 54 Pages: 351-357
  • NAID: 130004476837
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] New Methods for Clinical Proteomics in Allergy (2005)
  • Author(s): Kato, Z., Kondo, N
  • Journal Title: Allergology International 54 Pages: 351-357
  • NAID: 130004476837
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] New Methods for Clinical Proteomics in Allergy (2005)
  • Author(s): Kato Z, Kondo N
  • Journal Title: Allergology International 54 Pages: 351-357
  • NAID: 130004476837
• [Journal Article] The response of bovine beta-lactoglobulin-specific T-cell clones to single amino acid substitutions of T-cell core epitope.
  • Author(s): Kondo M, Kaneko H, Fukao T, Suzuki K, Sakaguchi H, Shinoda S, Kato Z, Matsui E, Teramoto T, Nakano T, Kondo N.
  • Journal Title: Pediatric Allergy and Immunology (In press)
  • Peer Reviewed
• [Presentation] MyD88タンパク立体構造析による新規シグナル伝達制御分子の開発 (2007)
  • Author(s): 大西秀典, 加藤善一郎, 李 愛蓮, 名田匡利, 相馬和佳, 松井永子, 金子英雄, 近藤直実, 杤尾豪人, 白川昌宏
  • Organizer: 日本アレルギー大会学会秋季学術大会(第57回)
  • Place of Presentation: 横浜
  • Year and Date: 2007-11-02
• [Presentation] MyD88タンパク立体構造解析による新規シグナル伝達制御分子の開発 (2007)
  • Author(s): 大西秀典, 加藤善一郎, 李 愛蓮, 名田匡利, 相馬和佳, 松井永子, 金子英雄, 近藤直実, 杤尾豪人, 白川昌宏
  • Organizer: 日本アレルギー大会学会秋季学術大会(第57回)
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Development of reguratory molecules based on the MyD88 structure (2007)
  • Author(s): Ohnishi, H., Kato, Z., Li, A., Nada, K., Samna, K., Matsui, E., Hideo, K., Rondo, N., Tochio, H., Shirakawa, M
  • Organizer: Annual Meeting of Japanese Society of Allergology
  • Place of Presentation: Yokohama. Japan
  • Description: 「研究成果報告書概要(欧文)」より