| Project/Area Number |
17591101
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
TSUTSUMI Hiroyuki Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (80217348)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAI Kazushige Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (50347168)
HONMA Shinjiro Sapporo Medical University, School of Medicine, Instructor, 医学部, 助手 (00404658)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Infection / Bronchial asthma / Allergy / 分子疫学 / RSウイルス / RSウイルスグループA / RSウイルスグループB |
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| Research Abstract |
The circulation pattern and genetic evolution of respiratory syncytial virus (RSV) in Japan were examined based on 109 RSV field strains isolated over 20 seasons (1980-2002) in two cities, Sapporo and Tokyo. The second hypervariable region of the large glycoprotein (G) gene was amplified by RT-PCR and the products sequenced directly. The nucleotide sequences were compared to those representatives of RSV genotypes identified previously. Japanese group A and B isolates clustered into five and four genotypes defined previously, respectively. Another one group A and one group B genotypes, which could not be assigned to previous genotypes, were also identified. Although different genotypes usually co-circulated in each season, the isolates in proximate seasons from two communities were usually located in the same branches. Moreover, the strains with genotypes defined previously were usually isolated at the same time as each reference strain of Western countries. Several mutant group B strains with 1-20 longer amino acid G proteins were newly identified in Sapporo. These findings suggest that Japanese RSV strains underwent geographical and also temporal clustering while participating in RSV genetic evolution in a global setting. In addition, Japanese strains, especially group B, might have evolved individually in each community, sometimes changing the length of the G protein.
Next, the relationship between RSV subgroup / genotype and the prognosis of RSV lower respiratory tract infection, that is recurrent wheezing or bronchial asthma were investigated. However, no apparent relationship of particular subgroup / genotype and these diseases.
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