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Research on the pathophysiology of dysmyelination using diffusion-weighted magnetic resonance imaging of mutant mice

Research Project

Project/Area Number 17591104
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionIbaraki Prefectural University of Health Sciences

Principal Investigator

IWASAKI Nobuaki  Ibaraki Prefectural University of Health Sciences, 付属病院, Associate professor (70251006)

Co-Investigator(Kenkyū-buntansha) MATSUMURA Akira  Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Department of Neurosurgery, Professor (90241819)
NITTA Naotaka  Ibaraki Prefectural University of Health Sciences, The National Institute of Advanced Industrial Science and Technology, Institute for Human Science & Biomedical Engineering, biomedical sensing and imaging group, Researcher (60392643)
NUMANO Shoichi  Tokyo Metropolitan University, Department of Radiological Science, Graduate School of Human Health Science, Assistant Professor (10399511)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsmyelination / dysmyelination / myelin basic protein / magnetic resonance imaging / diffusion weighted MRI / mutant mouse / shiverer mouse / 18q-syndrome / 磁気共鳴画像 / 拡散画像法 / 髄鞘形成 / MBP / 拡散画像
Research Abstract

Diffusion-weighted magnetic resonance imaging techniques were developed with MRI equipment specifically designed for small experimental animals. The diffusion-weighted images using a three-dimensional MP-RAGE MRI sequence, in addition to the conventional multislice spin-echo sequence, were performed on rats. Subsequently, the diffusion-weighted images and apparent diffusion coefficient maps were acquired in which a new saddle coil was adjusted for use on the mouse brain.
The temporal order of the myelination of the nerve tracts was quantitatively analyzed using immunohistochemistry with anti-myelin basic protein (MBP) antibody, anti-proteolipid protein (PLP) antibody and anti-myelin associated glycoprotein (MAG) antibody. This analysis was performed on the brains of wild-type, heterozygous mutant (MBPshi/+) and homozygous mutant (MBPshi/MBPshi) shiverer mice. No difference in the staining intensities of MBP, PLP and MAG was revealed between the wild-types and heterozygous mutants. On th … More e other hand, in the homozygous shiverer mutant, decreases in staining intensities for MBP and PLP were apparent, although MAG staining intensity appeared similar to the level observed in wild-type and heterozygous mice. Therefore, it seemed necessary to analyze the relationship between MBP and MAG.
A boy afflicted with chromosome 18 ring was investigated by brain MRI and autopsy examination. The T2 weighted image revealed diffuse high intensity signals throughout the cerebral white matter. The cerebral white matter was clearly described in the immunohistochemical staining for MBP and PLP.Electron microscopy revealed clusters of axons wrapped by compact myelin sheaths with distinct major dense lines. Holzer staining and immunohistochemical staining for GFAP revealed gliosis of the cerebral white matter.
It has previously been assumed that the high intensity signal of T2 weighted MRI images was caused by dysmyelination resulting from MBP gene abnormality. However, our pathological study demonstrated that the gliosis of cerebral white matter was an important cause for MRI abnormality instead of dysmyelination. We show the importance of using pathophysiological analysis in combination with MRI imaging in evaluating this mutant mouse as a valid animal model. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (11 results)

All 2008 2007 2006 2005

All Journal Article (9 results) Presentation (2 results)

  • [Journal Article] In vivo isotropic fat-saturated diffusion imaging of the ratpelvis using 3D MP-RAGE MR sequence2008

    • Author(s)
      Numano T, Homma K, Iwasaki N, Yagi K, Niitsu M, Abe S, Senoo A
    • Journal Title

      International Journal of Computer Assisted Radiology and Surgery 3・suppll

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] In vivo isotropic fat-saturated diffusion imaging of the rat pelvis using 3D MP-RAGE MR sequenceInternational2008

    • Author(s)
      Numano T, Homma K, Iwasaki N, Yagi K, Niitsu M, Abe S, Senoo A.
    • Journal Title

      Journal of Computer Assisted Radiology and Surgery 3・suppl1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fat-saturated diffusion-weighted imaging of the rat pelvisusing three-dimensional MP-RAGE MR sequence2007

    • Author(s)
      Numano T, Homma K, Iwasaki N, Hyodo K, Nitta N
    • Journal Title

      Conf Proc IEEE Eng Med Biol Soc 2007

      Pages: 2038-41

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fat-saturated diffusion-weighted imaging of the rat pelvis using three-dimensional MP-RAGE MR sequenceMRI2007

    • Author(s)
      Numano T, Homma K, Iwasaki N, Hyodo K, Nitta N.
    • Journal Title

      Conf Proc IEEE Eng Med Biol Soc 2007

      Pages: 2038-41

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] In vivo isotropic 3D diffusion tensor mapping of the rat brain using diffusion-weighted 3D MP-RAGE MRI.2006

    • Author(s)
      Numano T, Homma K, Iwasaki N, Hyodo K, Nitta N, Hirose T.
    • Journal Title

      Magn Reson Imaging 24・3

      Pages: 287-93

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] In vivo isotropic 3D diffusion tensor mapping of the rat brain using diffusion-weighted 3D MP-RAGE MRI2006

    • Author(s)
      Numano T, Homma K, Iwasaki N, Hyodo K, Nitta N, Hirose T
    • Journal Title

      Magn Reson Imaging 24-3

      Pages: 287-93

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] In vivo isotropic 3D diffusion tensor mapping of the rat brain using diffusion-weighted 3D MP-RAGE MRI2006

    • Author(s)
      Numano T, Homma K, Iwasaki N, Hyodo K, Nitta N, Hirose T
    • Journal Title

      Magn Reson Imaging 24

      Pages: 287-93

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Diffusion-weighted three-dimensional MP-RAGE MR imaging2005

    • Author(s)
      Numano T, Homma K, Hirose T
    • Journal Title

      Magn Reson Imaging 23・3

      Pages: 463-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Diffusion-weighted three-dimensional MP-RAGE MR imaging2005

    • Author(s)
      Numano T, Homma K, Hirose T.
    • Journal Title

      Magn Reson Imaging 23-3

      Pages: 463-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Fat-saturated Diffusion-weighted Imaging of the rat pelvis using three-dimensional MP-RAGE MR sequence2007

    • Author(s)
      T. Numano, K. Homma, N. Iwasaki, K. Hyodo, N. Nitta
    • Organizer
      29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
    • Place of Presentation
      Lyon, France
    • Related Report
      2007 Annual Research Report
  • [Presentation] 高速三次元Diffusion Tensor MRIの生体応用2007

    • Author(s)
      沼野智一、本間一弘、岩崎信明、疋島啓吾、廣瀬武志
    • Organizer
      第12回三次元CT・MRI研究会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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