| Project/Area Number |
17591116
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | KEIO UNIVERSITY |
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Principal Investigator |
SHIMADA Hiroyuki Keio University, School of Medicine, Assistant Professor, 医学部, 講師 (80265868)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Kimiko National Cancer Center Research Institute, Molecular Oncology Division, Staff Scientist, 分子腫瘍学部, 主任研究官 (00161414)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Pediatric Cancer / Susceptibility / SNP / DNA repair gene / Apoptosis |
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| Research Abstract |
Factors affected the susceptibility of pediatric malignant diseases may be innate phenomena. To explore this possibility, we tried a unique approach to analyze genetical and biological feature of lymphoblast cells established from the patients with childhood leukemia. Cells from the patients displayed marked chromosomal instability ; changes in ploidy, translocations and breakages. We re-sequenced entire coding regions in the ATM, CHK2, and TP53 genes in patients and healthy samples. The mutations in them were found only in the patients. Expression profiling shows abnormal expression of apoptosis and repair related genes. The result suggests germ-line defect(s) in repair or apoptosis pathways leads chromosomal aberrations and resulted in malignant transformation.
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