| Project/Area Number |
17591126
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kagoshima University (2006)
Kurume University (2005) |
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Principal Investigator |
KOSAI Kenichiro Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院医歯学総合研究科, 教授 (90258418)
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| Co-Investigator(Kenkyū-buntansha) |
MAENO Yasuki Kurume University, School of Medicine, Assistant Professor, 医学部, 講師 (90248401)
TAKEMURA Genzou Gifu University, Graduate School of Medicine, Assistant Professor, 大学院医学研究科, 講師 (40283311)
KUNISADA Takahiro Gifu University, Graduate School of Medicine, Professor, 大学院医学研究科, 教授 (30205108)
TAKAHASHI Tomoyuki Kurume University, School of Medicine, Associate Professor, 医学部, 助教授 (20332687)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Regenerative medicine / Gene / Development and differentiation / Transplantation |
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| Research Abstract |
We obtained the following results from the present research that established human ES cell-based technologies of regenerative medicine for pediatric diseases.
1. The development of cardiac differentiation
(1) Addition of growth factors in combination with embryoid body formation led to efficient cardiac differentiation of mouse ES cells. However, this method did not work well in the case of using human ES cells. Therefore, we move to another method.
(2) We screened several kinds of mesenchymal cells, and found that coculture with certain two types of mesenchymal cells led to successful cardiac differentiation of not only mouse ES cells but also human ES cells.
(3) We generated plasmid vectors that expressed cardiac-specific transcriptional factors, and transduced either of these to mouse ES cells. However, this method did not result in drastically efficient induction of cardiac differentiation. The results indicates that cardiac-specific transcriptional factors are not the master gene.
2. Purification of the ES cell-derived cardiac cells
We applied gene therapy vector technologies to in vitro differentiation systems using mouse ES cells, and developed novel method that efficiently isolated ES cell-derived target cells. Then, we introduce this method to human ES cell system, however, unexpectedly, this system did not visualize the target cells. After our careful studies, we found that the unknown characters of human ES cells that were different from mouse ES cells.
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