Physiological role of neurogrycan C, a protoglycan on a dendritic spine, on neuronal development
| Project/Area Number |
17591135
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Institute for Developmental Research, Aichi Human Service Center |
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Principal Investigator |
TOKITA Yoshihito Institute for Developmental Research, Aichi Human Service Center, Institute for Developmental Research Department of Perinatology, Senior scientist (50291175)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Chondroitinsulfate / Proteoglycan / synapse / spine / developmental disorder / 神経細胞 / フィロポディア |
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| Research Abstract |
Neuroglycan C is a membrane-spanning chondroitin sulfate proteoglycan involved in presynaptic formation in early postnatal stages in vivo. Neuroglycan C is located dendritic filopodia and spines both in vitro and in vivo. In this study, we found that a treatment of an antibody against neuroglycan C ectodomain translocates neuroglycan C to neuronal cell body from dendritic spine in primary cultured neuron. Numerous immunopositive vesicles were observed in cell soma of cultured neurons after the antibody treatment. The finding suggests thats neuroglycan C with dimmer formation could act as other trancemembrane type receptors for signaling molecule. Actually, a dimer form of neuroglycan C is detected with a western blotting of rat brain. Therefore, we investigated the effect of anti-neuroglycan C antibody on spine shape and neurite morphogenesis in vitro. The antibody collapses phalloidin positive growth cone and dendritic spines of cultured neuron. In addition to this, long term treatment of the antibody results in the reduction of MAP2 positive neurites, dendrites.
These results suggest that an unknown ligand for neuroglycan C that could interact with neuroglycan C ectodomain stimulates reorganization of cytoskeleton such as actin fiber and also microtubules via dimer formation of Neuroglycan C. Neuroglycan C dimer may activate intracellular signalings linking to neurite retraction. Endogenous ligand for neuroglycan C should be clarified to understand physiological function of neuroglycan C.
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Report
(4 results)
Research Products
(77 results)
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[Journal Article] Reduction of cerebral degeneration in neonatal hypoxic-ischemic rats by intracerebroventricular injection of neural stem/progenitor cells together with chondroitinase ABC.2008
Author(s)
Sato Y, Nakanishi K, Hayakawa M, Kakizawa H, Saito A, Kuroda Y, Ida M, Tokita Y, Aono S, Matui F, Kojima S, Oohira A
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Journal Title
J Neurochem 104
Pages: 1565-76
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ectodomain shedding of neuroglycan C, a brain-specific chondroitin sulfate proteoglycan, by TIMP-2- and TIMP-3-sensitive proteolysis.2007
Author(s)
Shuo T, Aono S, Nakanishi K, Tokita Y, Kuroda Y, Ida M, Matsui F, Maruyama H, Kaji T, Oohira A
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Journal Title
J Neurochem 102
Pages: 1561-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neuroprotective effect of nipradilol, an NO donor, on hypoxic-ischemic brain injury of neonatal rats.2007
Author(s)
Kakizawa H, Matsui F, Tokita Y, Hirano K, Ida M, Nakanishi K, Watanabe M, Sato Y, Okumura A, Kojima S, Oohira A
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Journal Title
Early Hum Dev 83
Pages: 535-40
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and functions of chondroitin sulfate in the milieu of neural stem cells2006
Author(s)
Ida, M, Shuo T, Hirano K, Tokita Y, Nakanishi K, Matsui F, Aono S, Fujita H, Kaji T, Oohira A
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Journal Title
J Biol Chem 281
Pages: 5982-91
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression and identification of a new splice variant of neuroglycan C, a transmembrane chondroitin sulfate proteoglycan, in the human brain.2006
Author(s)
Aono S, Tokita Y, Yasuda Y, Hirano K, Yamauchi S, Shuo T, Matsui F, Keino H, Kashiwai A, Kawamura N, Shimada A, Kishikawa M, Asai M and Oohira A
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Journal Title
J Neurosci Res 83
Pages: 110-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fates of Cdh23/CDH23 with Mutations Affecting the Cytoplasmic Region.2006
Author(s)
Yonezawa S, Yoshizaki N, Kageyama T, Takahashi T, Sano M, Tokita Y, Masaki S, Inaguma Y, Hanai A, Sakurai N, Yoshiki A, Kusakabe M, Moriyama A and Nakayama A
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Journal Title
Hum Mutat 27
Pages: 88-97
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Aono S,Kuroda Y,Suzuki A,Matsui F,Tokita Y,Nakanishi K,Ida M,Ikawa M,Okabe M,Ono K,Oohira A:Chondroitin sulfate of neuroglycan C,a CNS-specific transmembrane proteoglycan.2007
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[Presentation] Chondroitin sulfate of neuroglycan C, a CNS-specific transmembrane proteoglycan.2007
Author(s)
Aono S, Kuroda Y, Suzuki A, Matsui F, Tokita Y, Nakanishi K, Ida M, Ikawa M, Okabe M, Ono K, Oohira A
Organizer
21st Biennial Meeting of the International Society for Neurochemistry
Place of Presentation
Cancun, Mexico
Year and Date
2007-08-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A highly sulfated chondroitin sulfate preparation, CS-E, prevents excitatory amino acid-induced neuronal cell death.2006
Author(s)
Sato Y, Nakanishi K, Hayakawa M, Saito A, Kakizawa H, Ida M, Tokita Y, Matsui F, Aono S, Kojima S, Oohira A
Organizer
The 36th annual meeting of Society for Neuroscience
Place of Presentation
Atlanta, USA
Year and Date
2006-10-15
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Reduction of cerebral degeneration in neonatal hypoxic-ischemic rats by intracerebroventricular injection of neural stem/progenitor cells together with chondroitinase ABC.2006
Author(s)
Sato Y, Nakanishi K, Hayakawa M, Saito A, Kakizawa H, Ida M, Tokita Y, Matsui F, Aono S, Kojima S, Oohira A
Organizer
The 2nd Congress of Asian Society for Pediatric Research
Place of Presentation
Yokohama, Japan
Year and Date
2006-02-09
Description
「研究成果報告書概要(欧文)」より
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