|Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
The deposition of immune complexes induces an acute inflammatory response with tissue injury. Immune complex-induced tissue injury is mediated by inflammatory cell infiltration that is highly regulated by multiple chemokines. To assess the role of chemokine receptors, CCR1 and CCR5, and a ligand for these receptors, CCL3/macrophage inflammatory protein-1α, in this pathogenic process, the reverse passive cutaneous and peritoneal Arthus reaction was induced in mice lacking CCR1, CCR5, or CCL3. In the cutaneous Arthus reaction, edema was significantly attenuated in CCR1-deficient (CCR1^<-/->) and CCL3^<-/-> mice but not CCR5^<-/-> mice, compared with wild-type mice. Numbers of infiltrating neutrophils and mast cells were reduced in CCL3^<-/-> and CCR1^<-/-> mice, respectively, compared with wild-type mice. CCR1 and CCR5 were expressed on neutrophils and mast cells. Remarkably, the intradermal mRNA expression of CCL5/RANTES, another ligand for CCR1 and 5, was increased in CCR5^<-/-> and CCL3^<-/-> mice, compared with wild-type mice, while the cutaneous CCL3 mRNA expression was augmented in CCR1^<-/-> and CCR5^<-/-> mice. These results indicate that CCR1, CCR5, and CCL3 cooperatively contribute to the cutaneous Arthus reaction and also suggest that enhanced expression of CCL3 and CCL5 compensates for the loss of CCR1, CCR5, and CCL3 in the reaction.