The dynamics of epiplakin null keratinocytes and the production of transgenic mice of epiplakin
| Project/Area Number |
17591176
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Oita University |
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Principal Investigator |
FUJIWARA Sakuhei Oita University, Faculty of Medicine, Professor, 医学部, 教授 (90181411)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Osamu Oita University, Faculty of Medicine, Lecturer, 医学部, 講師 (40284799)
SATO Haruaki Oita University, Faculty of Medicine, Assistant, 医学部, 助手 (20363559)
UCHIDA Tomohisa Oita University, Faculty of Medicine, Assistant, 医学部, 助手 (70381035)
SUMIYOSHI Hideaki Oita University, Faculty of Medicine, Assistant, 医学部, 助手 (60343357)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Bullous Pemphigoid / plectin / hemidesmosome / autoantiger / keratinocyte / intermediate filament / cell attachment / epiplakin / 基底膜 |
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| Research Abstract |
Abstract
Epiplakin, a cytoskeletal linker protein, was originally identified as an autoantigen in a serum spec men obtained from a patient with subepidermal blistering disease. To examine the binding ability of epiplakin with intermediate filaments (IFs), we performed slot-blot assays using fusion proteins that included various domains and subdomains of epiplakin. At least two of the 4.6 copies in the B domains of epiplakin were necessary for the binding of fusion proteins to keratin. The repeated structures of linker domains also played an important role in the binding of epiplakin to keratin in these assays while also increasing the repeated structure in the linker domain of epiplakin which is involved in the increased binding to IFs. A similar but weaker binding to vimentin and desmin was also detected. These observations indicated that the highly repeated structures of epiplakin in both the B and the linker domains, which is the unique feature of this molecule in the plakin family, play an essential role in the functioning of this molecule.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Epiplakin, a novel member of the plakin family originally identified as a 450-kDa human epidermal autoantigen : Structure and tissue localization.2001
Author(s)
Fujiwara.S, Takeo.N, Otani.Y, Parry.DAD, Kunimatsu.M, Lu.R, Sasaki.M, Matsuo.N, Khaleduzzaman.M, Yoshioka.H
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Journal Title
J Biol Chem 276
Pages: 13340-13347
Description
「研究成果報告書概要(欧文)」より
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