Analysis of methylation status of the promoter of the IRF2 gene, a candidate gene for psoriasis
| Project/Area Number |
17591180
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Osaka City University |
|---|
Principal Investigator |
FUKAI Kazuyoshi Osaka City University, Graduate School of Medicine, Associate professor, 大学院医学研究科, 助教授 (20244642)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | psoriasis / interferon / transcription factor / methylation / promoter / インターフェロン / エピジェネティックス |
|---|
| Research Abstract |
The interferon regulatory factor 2 gene is a strong candidate gene for type 2 psoriasis, because of the localization of the gene to chromosome 4q35, and the function of the protein to negatively regulate the secretion of interferon alpha and beta. The methylation of the promoter alters the expression of the gene and has been implicated as the cause for aging and cancer. The methylation status of the interferon regulatory factor 2 was investigated in DNA of peripheral leukocytes with type 2 psoriasis. Genomic DNAs of ten cases with old psoriasis were bisulfated. The promoter region of the IRF2 gene was PCR-amplified and cloned to TA cloning vector and sequenced. Approximately 20 clones were sequenced with each subject. However, it was demonstrated that the promoter of the IRF2 gene was not methylated in type 2 psoriasis. The proportion of the methylated CpG was about 0.5%. Thus, it was suggested that the methylation of the promoter of IRF2 gene was not associated with type 2 psoriasis.
|
Report
(3 results)
Research Products
(23 results)
