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Development of quantitative analyzing method for phase X-ray CT images of biological objects

Research Project

Project/Area Number 17591244
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionUniversity of Tsukuba

Principal Investigator

WU Jin  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant Professor, 大学院人間総合科学研究科, 講師 (20375512)

Co-Investigator(Kenkyū-buntansha) TAKEDA Tohoru  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant Professor, 大学院人間総合科学研究科, 講師 (10197311)
YUASA Tetsuya  Yamagata University, Faculty of Engineering, Professor, 工学部, 教授 (30240146)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsSynchrotron radiation / X-ray interferometer / Phase-contrast X-ray CT / Biomedical imaging / Oncology / Alzheimer's disease / Kidney / Animal model of human disease / 生体イメージング / 血管 / 癌 / 小動物 / 形態画像 / 定量化
Research Abstract

Currently, X-ray micro-imaging techniques for small laboratory animal models of human diseases are an exciting research topic. To obtain the fine morphological information, it is inevitable to take on the optimal scale of imaging modality on small animals, and high-contrast and high-spatial resolutions are required. X-ray phase-contrast imaging techniques with an X-ray interferometer, which has about 1000-times higher contrast sensitivity for the light atomic elements than conventional X-ray absorption-contrast method, enables us to enhance the contrast of biological soft tissue without a contrast agent. The aim of this study is to develop a three-dimensional quantitative analyzing method for the evaluation of the physiological states of small animals.
The X-ray phase-contrast CT (PCCT) system consists of a triple Laue-case X-ray interferometer, a phase shifter, a sample cell and an X-ray CCD camera. The analyzer thickness was 1mm at the PF of the High Energy Accelerator Research Organi … More zation (KEK), and 0.04 mm at 20XU of SPring-8 respectively. The pixel size of the detector was 0.018mm x 0.018mm at PF of KEK, and 0.004 mm x 0.004 mm at SPring-8 respectively.
The formalin-fixed biological samples of human diseases models, such as colon cancer implanted in nude mice, the brain with Alzheimer's disease in mice, and the kidney of focal segmental glomerulosclerosis in hamsters, are imaged. 3D PCCT images could clearly demonstrate various pathological changes and anatomical structures of colon cancer specimens without a contrast agent. In the case of Alzheimer's disease, the deposition of B amyloid was visualized in the cortex and the hippocampus, and its number and size of individual B amyloid plaque were analyzed quantitatively. In the kidney, glomeruli and tubular, their structures were observed as similar images to 40 or 100-time magnified optical microscope images by our micro-PCCT. The distribution of renal microvasculature, and the number and size of glomeruli were successfully depicted and observed. The density of renal tissue, and glomerular volume were also analyzed quantitatively. Our results suggest that the quantitative PCCT image analysis could provide important biomedical information for small animal research. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (34 results)

All 2007 2006 2005

All Journal Article (34 results)

  • [Journal Article] Validity of fusion imaging of hamster heart obtained by fluorescent and phase-contrast X-ray CT with synchrotron radiation.2007

    • Author(s)
      Jin Wu
    • Journal Title

      Proc. AIP CP 879

      Pages: 1956-1959

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interferometer-based phase-contrast X-ray CT of colon cancer specimens : Comparative study with 4.74 tesla MRI and optical microscopy.2007

    • Author(s)
      Tohoru Takeda
    • Journal Title

      J. Computed Assist Tomography 31

      Pages: 214-217

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Validity of fusion imaging of hamster heart obtained by fluorescent and phase-contrast X-ray CT with synchrotron radiation.2007

    • Author(s)
      Wu J, et al.
    • Journal Title

      Proc.AIP CP 879

      Pages: 1956-1959

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interferometer-based phase-contrast X-ray CT of colon cancer specimens : Comparative study with 4.74 tesla MRI and optical microscopy.2007

    • Author(s)
      Takeda T, et al.
    • Journal Title

      J.Computed Assist Tomography 31

      Pages: 214-217

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] validity of fusion imaging of hamster heart obtained by nuorescent and phase-contrast X-ray CT with synchrotron radiation.2007

    • Author(s)
      Jin Wu
    • Journal Title

      Proc.AIP CP 879

      Pages: 1956-1959

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Micro-coronary vessel imaging using phase-contrast X-ray computed tomography.2006

    • Author(s)
      Jin Wu
    • Journal Title

      Proc. 12^<th> World Congress on Heart Disease

      Pages: 171-174

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Fusion imaging of fluorescent and phase-contrast x-ray Computed tomography using synchrotron radiation in medical biology.2006

    • Author(s)
      Jin Wu
    • Journal Title

      Proc. SPIE 6318

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Progress in biomedical application of phase-contrast x-ray imaging and fluorescent x-ray CT.2006

    • Author(s)
      Tohoru Takeda
    • Journal Title

      Proc. SPIE 6318

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantitative analysis of amyloid plaques in a mouse model of alzheimer's disease by phase-contrast X-ray computed tomography.2006

    • Author(s)
      Kyoko Noda-Saita
    • Journal Title

      Neuroscience 138

      Pages: 1205-1213

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 3D myocardial morphological observation of cardiomyopathic hamster by phase-contrast X-ray CT.2006

    • Author(s)
      Thet-Thet-Lwin
    • Journal Title

      Proc. 12^<th> World Congress on Heart Disease

      Pages: 181-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Micro-coronary vessel imaging using phase-contrast X-ray computed tomography.2006

    • Author(s)
      Wu J, et al.
    • Journal Title

      Proc.12^<th> World Congress on Heart Disease

      Pages: 171-174

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Fusion imaging of fluorescent and phase-contrast x-ray Computed tomography using synchrotron radiation in medical biology.2006

    • Author(s)
      Wu J, et al.
    • Journal Title

      Proc.SPIE 6318

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Progress in biomedical application of phase-contrast x-ray imaging and fluorescent x-ray CT.2006

    • Author(s)
      Takeda T, et al.
    • Journal Title

      Proc.SPIE 6318

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantitative analysis of amyloid plaques in a mouse model of alzheimer's disease by phase-contrast X-ray computed tomography.2006

    • Author(s)
      Noda-Saita K, et al.
    • Journal Title

      Neuroscience 138

      Pages: 1203-1213

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 3D myocardial morphological observation of cardiomyopathic hamster by phase-contrast X-ray CT.2006

    • Author(s)
      Thet-Thet-Lwin, et al.
    • Journal Title

      Proc.12^<th> World Congress on Heart Disease

      Pages: 181-184

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Fusion imaging of fluorescent and phase-contrast X-ray Computed tomography using synchrotron radiation in medical biology.2006

    • Author(s)
      Jin Wu
    • Journal Title

      Proc.SPIE 6318

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Progress in biomedical application of phase-contrast x-ray imaging and fluorescent x-ray CT.2006

    • Author(s)
      Tohoru Takeda
    • Journal Title

      Proc.SPIE 6318

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Quantitative analysis of amyloid plaques in a mouse model of alzheimer's desease by phase-contrast X-ray computed tomography.2006

    • Author(s)
      Kyoko Noda-Saita
    • Journal Title

      Neuroscience 138

      Pages: 1205-1213

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Integrated imaging of mouse brain with fluorescent and phase-contrast X-ray CT.2005

    • Author(s)
      Jin Wu
    • Journal Title

      Proc. 6^<th> Asian-Pacific Conference on Medical and Biological Engineering PA-3-37

      Pages: 1-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Evaluation of mouse brain fused image obtained from fluorescent and phase-contrast X-ray computed tomography using synchrotron radiation.2005

    • Author(s)
      Jin Wu
    • Journal Title

      Medical Imaging Technology 23

      Pages: 312-317

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coherence-contrast x-ray imaging based on x-ray interferometry.2005

    • Author(s)
      Akio Yoneyama
    • Journal Title

      Applied Optics 44

      Pages: 3258-3261

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] High-energy phase-contrast X-ray imaging using two-crystal X-ray interferometer.2005

    • Author(s)
      Akio Yoneyama
    • Journal Title

      J. Synchrotron Radiation 12

      Pages: 534-536

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Biomedical imaging by X-ray phase tomography using diffraction-enhanced imaging.2005

    • Author(s)
      Ichirou Koyama I
    • Journal Title

      Jpn J Appl Phys 44

      Pages: 8219-8221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Integrated imaging of mouse brain with fluorescent and phase-contrast X-ray CT.2005

    • Author(s)
      Jin Wu, et al.
    • Journal Title

      Proc.6^<th> Asian-Pacific Conference on Medical and Biological Engineering PA-3-37

      Pages: 1-4

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Evaluation of mouse brain fused image obtained from fluorescent and phase-contrast X-ray computed tomography using synchrotron radiation.2005

    • Author(s)
      Wu J, et al.
    • Journal Title

      Medical Imaging Technology 23

      Pages: 312-317

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Coherence-contrast x-ray imaging based on x-ray interferometry.2005

    • Author(s)
      Yoneyama A, et al.
    • Journal Title

      Applied Optics 44

      Pages: 3258-3261

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] High-energy phase-contrast X-ray imaging using two-crystal X-ray interferometer.2005

    • Author(s)
      Yoneyama A, et al.
    • Journal Title

      J.Synchrotron Radiation 12

      Pages: 534-536

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Biomedical imaging by X-ray phase tomography using diffraction-enhanced imaging.2005

    • Author(s)
      Koyama I, et al.
    • Journal Title

      Jpn J Appl Phys 44

      Pages: 8219-8221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Overview of clinical intravenous coronary angiography both in Japan and at ESRF.2005

    • Author(s)
      Ohtsuka S, et al.
    • Journal Title

      Nucl Instrum Meth A 548

      Pages: 78-83

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 蛍光および位相X線CTを用いたマウス脳融合画像の有用性2005

    • Author(s)
      呉 勁
    • Journal Title

      Medical Imaging Technology 23

      Pages: 312-317

    • NAID

      10016865075

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Phase-contrast and fluorescent X-ray imaging for biomedical researches.2005

    • Author(s)
      Tohoru Takeda
    • Journal Title

      Nuclear Instruments and Methods in Physics Research A 548

      Pages: 38-46

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cerebral Perfusion imaging of live mice by fluorescent x-ray CT.2005

    • Author(s)
      Tohoru Takeda
    • Journal Title

      Proc. IEEE ICIP 2005

    • Related Report
      2005 Annual Research Report
  • [Journal Article] In-vivo element imaging with fluorescent X-ray computed tomography.2005

    • Author(s)
      Tohoru Takeda
    • Journal Title

      Proc. 6^<th> Asian-Pacific Conference on Medical and Biological Engineering PA-3-33

      Pages: 1-4

    • Related Report
      2005 Annual Research Report
  • [Journal Article] High-energy phase-contrast X-ray imaging using two-crystal X-ray interferometer.2005

    • Author(s)
      Akio Yoneyama et al.
    • Journal Title

      J. Synchrotron Radiation 12

      Pages: 534-536

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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