Apoptosis in myocardial ischemia and remodeling and apostolic imaging
| Project/Area Number |
17591253
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kanazawa University |
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Principal Investigator |
TAKI Junichi Kanazawa University, Hospital, Instructor (10251927)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Kenichi Kanazawa University, Graduate School of Medicine, Associate Professor (00167545)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | apoptosis / myocardial infarction / Tc-99m-annexin V / reoerfusion / remodeling / myocardial ischemia / Tm-99m-annexin V |
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| Research Abstract |
To determine whether cardio-protective interventions such as ischemic preconditioning, postconditioning, and suppression of apoptosis by caspase inhibitor suppress myocardial apoptosis, Tc-99m-annexin V (Tc-AV) imaging was performed in a rat model of 20min ischemia and reperfusion. After 20min of coronary occlusion and reperfusion, Tc-AV was injected at 30min and 90min after reperfusion. One hour later the coronary artery was re-occluded and thallium-201 was injected to delineated area at risk and the rats were sacrificed 1 min later. Dual tracer autoradiography was performed to assess Tc-AV uptake and area at risk. In control rats, significant Tc-AV uptake was observed within ischemic area (Tc-AV uptake ratio (ischemic to normal area's count density ratio) was 4.15±1.89, 3.70±1.41 at 30min and 90min after reperfusion, respectively). In rats with ischemic preconditioning the uptake ratio decreased significantly (1.17±0.29, 1.33±0.74, respectively). By postconditioning the Tc-AV uptake was also suppressed (2.09±0.56, 1.88±0.69, respectively). Caspase inhibitor treatment also reduced the uptake (2.08±0.50, 1.27±0.24, respectively). Two weeks after reperfusion, Tc-AV uptake was not observed in rats without myocardial thinning (1.01± 0.29), however, the significant Tc-AV uptake was observed in the area of wall thinning or remodeling (1.71± 0.34). In conclusion, myocardial remodeling after ischemia may be related to apoptosis. Apoptosis can be suppressed by the cardio-protective interventions and Tc-AV imaging may be a way to monitor myocardial injury and its response to novel therapeutic interventions including postconditioning, preconditioning and antiapoptotic therapy.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Effect of postconditioning on myocardial 99mTc-annexin-V uptake : comparison with ischemic preconditioning and caspase inhibitor treatment2007
Author(s)
Taki, J., Higuchi, T., Kawashima, A., Fukuoka, M., Kayano, D., Tait, JF., Matsunari, I., Nakajima, K., Kinuya, S., Strauss, HW
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Journal Title
Journal of Nuclear Medicine 48
Pages: 1301-1307
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] 99m Tc-Annexin-V uptake in a rat model of variable ischemic severity and reperfusion time2007
Author(s)
Taki, J., Higuchi, T., Kawashima, A., Fukuoka, M., Kayano, D., Tait, JF., Matsunari, I., Nakajima, K., Kinuya, S., Strauss, HW
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Journal Title
Ciculation Journal 71
Pages: 1141-1146
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ischemic post-conditioning reduces Tc-99m-annexin V uptake in rat hearts2006
Author(s)
Taki, J., Higuchi, T., Matsunari, I., Kawano, M., Nakjima, K., Tonami, N
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Journal Title
Ciculation Journal 70
Pages: 5114-5115
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Time course of discordant BMIPP and thallium uptake after ischemia and reperfusion in a rat model.2005
Author(s)
Higuchi, T., Taki, J., Nakajima, K., Kinuya, S., Namura, M., Tonami, N
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Journal Title
Journal of Nuclear Medicine 46
Pages: 172-175
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 9mTc-annexin V Uptake Reduction by Caspase Inhibitor and Ischemic Preconditioning in A Rat Model of Myocardial Ichemia and Reperfusion2005
Author(s)
Taki J, Higuchi T, Kawashima A, Muramori A, Fukuoka M, Kayano D, Nakajima K. Matsunari I, Jean-Luc Vanderheyden, Tait JF, Strauss HW, Tonami N
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Journal Title
Related Report
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[Presentation] Postconditioning attenuates myocardial Tc-99m-annexin-V uptake in rats with ischaemia and reperfusion : comparison with ischemic preconditioning and caspase inhibitor treatment2007
Author(s)
Taki, J., Higuchi, T., Kawashima, A., Matsunari, I., Nakajima, K., Vanderheyden, JL, Tait, JF., Strauss, HW
Organizer
8th international Conferences of Nuclear Cardiology
Place of Presentation
Prague
Year and Date
2007-05-02
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effect of ischemic post-conditioning on Tc-99m-annexin-V uptake in rats with ischemia and reperfusion : Comparison with preconditioning and caspase inhibitor2006
Author(s)
Taki, J., Higuchi, T., Kawashima, A., Tait, JF., Matsunari, I., Nakajima, K., Vanderheyden, J., Tonami,N., Strauss, HW
Organizer
53rd annual meeting of the Society of Nuclear Medicine
Place of Presentation
San Diego
Year and Date
2006-06-06
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Ischemic post-conditioning reduces Tc-99m-annexin V uptake in rat hearts2006
Author(s)
Taki, J., Higuchi, T., Matsunari, I., Kamano, M., Nakajima, K., Tonami, N
Organizer
The 70th Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
Nagoya
Year and Date
2006-03-24
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 99m Tc-annexin V Uptake Reduction by Caspase Inhibitor and Ischemic Preconditioning in A Rat Model of Myocardial Ichemia and Reperfusion2005
Author(s)
Taki J, Higuchi T, Kawashima A, Muramori A, Fukuoka M, Kayano D, Nakajima K, Matsunari I, Jean-Luc Vanderheyden, Tait JF, Strauss HW, Tonami N
Organizer
第45回日本核医学会学総会
Place of Presentation
船堀
Year and Date
2005-11-11
Description
「研究成果報告書概要(和文)」より
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[Presentation] Jean-Luc Vanderheyden, Tait JF, Strauss HW Tonami N, 99mTc-annexin V Uptake Reduction by Caspase Inhibitor and Ischemic Preconditioning in A Rat Model of Myocardial Ichemia and Reperfusion2005
Author(s)
Taki, J., Higuchi, T., Kawashima, A., Muramori, A., Fukuoka, M., Kayano, D., Nakajima, K., Matsunari, I
Organizer
45 th Annual Meeting of the Japanese Society of Nuclear Medicine
Place of Presentation
Funabori
Year and Date
2005-11-11
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Relationship between apoptosis and ischemic severity in rats : Assessment by Tc-99m-annexin V2005
Author(s)
Taki, J., Higuchi, T., Muramori, A., Fukuoka, M., Kayano, D., Nakajima, K., Matsunari, I., Tonami, N
Organizer
61st Tyubu Regional Conferences, Society of Japanese Nuclear Medicine
Place of Presentation
Toyama
Year and Date
2005-07-02
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Tc-99m-annexin V uptake depends on ischemic severity and reperfusion time2005
Author(s)
Taki J, Higuchi T, Kawashima A, Nakajima K, Muramori A, Matsunari I, Tait JF, Vanderheyden JL, Strauss HW, Tonami N.
Organizer
7th international Conferences of Nuclear Cardiology
Place of Presentation
Lisbon, Portugal
Year and Date
2005-05-10
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Tc-99m-annexin V uptake depends on ischemic severity and reperfusion time2005
Author(s)
Taki, J., Higuchi, T., Kawashima, A., Nakajima, K., Muramori, A., Matsunari, I., Tait, JF., Vanderheyden, JL, Strauss, HW., Tonami, N
Organizer
7th international Conferences of Nuclear Cardiology
Place of Presentation
Lisbon
Year and Date
2005-05-10
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Ischemic preconditioning reduces Tc-99m-annexin V uptake in rat hearts2005
Author(s)
Taki, J., Higuchi, T., Matsunari, I., Kawano, M., Nakajima, K., Tonami, N
Organizer
The 69th Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
Yokohama
Year and Date
2005-03-20
Description
「研究成果報告書概要(欧文)」より
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