| Co-Investigator(Kenkyū-buntansha) |
FUKUMORI Yoshinobu Kobe Gakuin University, The Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (60102927)
HIRATUKA Junichi Kawasaki Medical School, Department of Radiation Oncology, Associate Professor, 放射線医学(治療), 助教授 (30192298)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
TRP-2 gene is specifically expressed in melanoma cells, and is thought to related to the radio-resistance of melanoma. The aim of this project is to establish the method of improvement of radiotherapy for melanoma by suppression of TRP-2 gene expression, cultured melanoma cell level experiments were performed as the first step.
The results obtained through this research project are summarized as follows :
1. TRP-2 sh-RNA expression plasmids were transfected to tyrosinase-ecpression deficient amelanotic melanoma cell to construct TRP-2 expression deficient melanoma cell lines.
2. Evaluation systems for the killing effect of X-ray, UV, and cisplatin were established to the effect of TRP-2 expression in melanoma cells.
3. Evaluation system for measuring the content of melanin monomer DHICA, which is the product of TRP-2, was established.
4. Inversely, to evaluate the effect of TRP-2 expression in cells, non-melanoma cultured cell, HeLa cells were transfected with TRP-2 expression plasmid. Using this cell line we can clarify whether the radiation-resistance of melanoma is related to the expression and production of TRP-2 protein or melanogenesis related to TRP-2. We evaluated the properties of these cell lines concerning melanogenesis, cell biology, and molecular biology.
Comparing the these properties of established cell lines, we could not obtained clear results that TRP-2 gene suppression improve the killing effect of X-ray radiation, UV, and cisplatin. Currently, we improve the evaluation systems and methods, and continue to the evaluation of the effects.
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