| Co-Investigator(Kenkyū-buntansha) |
TAMADA Tsutomu Kawasaki Medical School, Lecturer, 医学部, 講師 (40278932)
YAMASHITA Takenori Kawasaki Medical School, Lecturer, 医学部, 講師 (30299200)
TOKIYA Ryoji Kawasaki Medical School, Assistant, 医学部臨床, 助手 (80309559)
UMETANI Keiji Japan Synchrotron Radiation Research Institute, Senior Scientist, 実験部門主幹, 研究員 (50344396)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
Purpose: Our studies aimed to evaluate the structures of micro vessels in tumor using monochromatic X-ray 3D-CT.
Materials and Methods: VX2 carcinomas were transplanted into the 50 auricles of rabbits, and randomize into 5 groups. To the first group, 12 hours after transplantation, we injected Ba-gelatine mixed emulsion into the auricular artery by hands. In the same way, to the second group we injected Ba-gelatine mixed emulsion 24 hours after injection, to the third 48 hours, to the fourth 72 hours, to the fifth after 7 days. First of all, we take two dimensional projections of the auricles using monochromatic x-ray, and select several auricles, had moderately consistency of vessels. Subsequently, 4 auricle were taken 3D-CT images. One is taken images in 3 different parameters to decide the adequate parameters. And, other 3 were take different period after injection to decide the adequate period after injection. Image analysis was done with Image-Pro Plus, TRI-3D VOL, and visually.
Results: Because of a great number of images, reconstruction of images has not finished. So numerical analysis has not been archived. In visually, the images, in 5.83 um/pixel is obviously clear rather than in 12 um/pixel. There is no differentiation between the image by 20KeV and by 36KeV. And most adequate period to evaluate the vessels seems to be 72 hours after transplantation.
Conclusion: The monochromatic X-ray 3D-CT system was a useful tool to evaluate the tumor microvessels. But to take images and to reconstruction images needs too much time.
After this, we are going to analyze the images immediately.
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