Development of cultured skin cells bearing enhanced antimicrobial activity
| Project/Area Number |
17591348
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Tokai University |
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Principal Investigator |
INOKUCHI SADAKI Tokai University, SCHOOL OF MEDICINE, PROFESSOR (60160008)
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| Co-Investigator(Kenkyū-buntansha) |
ITO ISAO TOKAI UNIVERSITY, 医学部, LECTURER (70297258)
UMEZAWA Kazuo TOKAI UNIVERSITY, 医学部, LECTURER (30349344)
SEKI TOMOKO TOKAI UNIVERSITY, 医学部, LECTURER (00316605)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,610,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | antimicrobial peptide / cultured skin / eene transfer / gene therapy / burns / defensin / MRSA / MDRP / 移植、再生医療 / ウイルス / ヒト抗菌ペプチド / ウイルスベクター / 表皮細胞 / 線維芽細胞 |
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| Research Abstract |
Purpose : Tb enhance the antimicrobial activity of cultured human skin cells by the introduction of human beta defensin genes.
Materials and Methods: cDNA of human beta defensin (hBD) 2 and 3 was cloned by the RT-PCR method. Recombinant adenovirus vectors encoding hBD2 or hBD3 cDNA driven by the CMV promoter were constructed. Cultured human keratinocytes or dermal fibroblasts were infected with each of the recombinant viruses. The supernatants of the virus-infected cells were examined for bactericidal activity against S.aureus, multi-drug resistant S.aureus (MRSA) , E.coli, P.aeruginosa and multi-drug resistant P.aeruginosa (MDRP)
Results: The supernatant of the hBD3-introduced cells showed high bactericidal activity against S.aureus and MRSA, but little, activity against E. coli, P.aeruginosa and multi-drug resistant P aeruginosa (MDRP) . The supernatant of the hBD2-introduced cells showed weak or no bactericidal activity.
Conclusion : The antimicrobial activity of cultured human skin cells against S.aureus, including MRSA, was enhanced by the introduction and overexpression of the hBD3 gene.
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Report
(4 results)
Research Products
(11 results)
