Serum anti-myomeglin antibodies is a useful tumor marker to detect gastrointestinal cancer

• Project/Area Number: 17591370
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Chiba University
• Principal Investigator: SHIMADA Hideaki Chiba University, GRADUATE SCHOOL OF MEDICINE, ASSISTANTPROFESSOR, 大学院医学研究院, 講師 (20292691)
• Co-Investigator(Kenkyū-buntansha): HIWASA Takaki Chiba University, GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 大学院医学研究院, 助教授 (30260251) ; OCHIAI Takenori Chiba University, GRADUATE SCHOOL OF MEDICINE, 大学院医学研究院, 教授 (80114255)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000); Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: SEREX / Esophageal cancer / Squamous cell carcinoma / tumor marker / prognosis / myomegalin / 胃癌 / 大腸癌 / 癌抗原 / IgG抗体

Research Abstract

In order to identify new serum markers of esophageal squamous cell carcinoma (SCC), we performed serological identification of antigens by recombinant cDNA expression cloning (SEREX). E. coli was infected with λZAPII phage cDNA library prepared from mRNA of an esophageal cancer cell line (T.Tn), and IPTG-induced cDNA products were screened for interaction with antibodies in allogeneic sera of patients with esophageal SCC. We identified myomegalin (phosphodiesterase 4D interacting protein/PDE4DIP) as a new SEREX antigen for esophageal SCC. Western blotting analysis revealed that serum anti-myomegalin antibodies (s-myomegalin-Abs) were present in 43 out of 91 (47%) patients but in only one out of 45 (2.2%) healthy controls. Anti-myomegalin Abs were present 38% of stage I patients. The positive rate of s-myomegalin-Abs was the highest among the other conventional tumor markers. Although the presence of s-myomegalin-Abs was not related to any of clinicopathological features of the patients concerned, multivariate analysis indicated that presence of s-myomegalin-Abs was significantly associated with favorable prognosis

Research Products

• [Journal Article] Serum anti-myomegalin antibodies in patients with esophageal squamous cell carcinoma (2007)
  • Author(s): Shimada, H., Kuboshima, M., Shiratori, T., Nabeya, Y., Takeuchi, A., Takagi, H., Nomura, F., Takiguchi, M., Ochiai, T., Hiwasa, T
  • Journal Title: International Journal of Oncology 30 Pages: 97-103
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Serum anti-myomegalin antibodies in patients with esophageal squamous cell carcinoma (2007)
  • Author(s): Shimada, H., Kuboshima, M., Shiratori, T., Nabeya, Y., Takeuchi, A., Takagi, H., Nomura, F., Takiguchi, M., Ochiai, T., Hiwasa, T.
  • Journal Title: International Journal of Oncology Pages: 3097-3103
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Serological identification of antigens by recombinant cDNA expression cloning (SEREX) using antibodies from patients with esophageal squamous cell carcinoma (2006)
  • Author(s): Hiwasa, T., Shimada, H., Ochiai, T., Takiguchi
  • Journal Title: Moleculomics and Thereafter Pages: 99-117
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Serological identification of antigens by recombinant cDNA expression cloning (SEREX) using antibodies from patients with esophageal squamous cell carcinoma (2006)
  • Author(s): Hiwasa, T., Shimada, H., Ochiai, T., Takiguchi
  • Journal Title: Moleculomics and Thereafter 26 Pages: 99-117
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An essential role of alternative splicing of c-myc suppressor FUSE-binding protein-interacting repressor in carcinogenesis. (2006)
  • Author(s): Matsushita K, Tomonaga T, Shimada H, Shioya A, Higashi M, Matsubara H, Harigaya K, Nomura F, Libutti D, Levens D, Ochiai T.
  • Journal Title: Cancer Res. 66(3) Pages: 1409-1417
• [Journal Article] Proteomic analysis of primary esophageal squamous cell carcinoma reveals down regulation of a cell adhesion protein, periplakin. (2006)
  • Author(s): Nishimori T, Tomonaga T, Matsushita K, Oh-Ishi M, Kodera Y, Maeda T, Nomura F, Matsubara H, Shimada H, Ochiai T.
  • Journal Title: Proteomics 6(3) Pages: 1011-1018
• [Journal Article] Identification of a novel SEREX antigen, SLC2A1/GLUT1, in esophageal squamous cell carcinoma. (2006)
  • Author(s): Kuboshima M, Shimada H, Liu TL, Nakashima K, Nomura F, Takiguchi M, Hiwasa T, Ochiai T.
  • Journal Title: Int J Oncol. 28(2) Pages: 463-468