| Project/Area Number |
17591379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KARAKO Takashi The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (00313213)
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| Co-Investigator(Kenkyū-buntansha) |
MAKUUCHI Masatoshi The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (60114641)
KOKUDO Norihiro The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (00205361)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Cholangiocarcinoma / Clinicopathology / Glycocojugates / Mucin / Liver cancer / Immunohistochemistry / Tumor marker / Diagnosis / 糖鎖 |
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| Research Abstract |
Patients with cholangiocarcinoma (CC) has particularly worse prognosis than those with hepatocellular carcinoma (HCC), which is due to the difficulty of the diagnosis of CC in many cases. Thus, it is important to develop an effective tumor marker that can diagnosis CC in early phase and estimate patients' prognosis. In this study, we aimed to elucidate significance of KL-6 mucin in CC patients by immunohistochemical and seralogical analyses. The histochemical analysis showed that positive staining of KL-6 mucin was detected only in CC tissues but not in HCC tissues and normal liver regions of all liver cancer tissues. Serological analysis showed serum KL-6 mucin level was elevated in CC patients compared with healthy individuals, HCC patients and metastatic liver cancer patients (P<0.0001) and that CC patients were able to be discrminate from other liver cancer patients by setting a cut-off value at 248 U/mL. These results indicated that KL-6 mucin might be useful in the histochemical detection and serological discrimination of CC patients. Furthermore, the immunohistochemical analysis of metastatic liver cancer tissues displayed that all tissues were positive for KL-6 mucin, but the characteristics of KL-6 expression is not the same as CC tissues as well as serum KL-6 mucin level. Moreover, experiment using various kinds of colon cancer lines showed that the localization of KL-6 mucin was significantly related to the cancer behavior such as cancer cell morphology and metastatic potential. The present study indicates that KL-6 is prospective tool for CC diagnosis and elucidation of various gastrointestinal cancer behaviors.
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