| Project/Area Number |
17591396
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
TAKEMASA Ichiro Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (50379252)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Hirofumi Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (30322184)
IKEDA Masataka 0saka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (80335356)
SEKIMOTO Mitsugu Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (10273658)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | colorectal cancer / DNA chip / gene expression profile / molecular diagnostic system / endoscopic biopsy / 遣伝子診断システム |
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| Research Abstract |
The aim of this study is to establish the preoperative molecular diagnostic system for colorectal cancer using endoscopic biopsies and to apply the system into clinic with the improvement of technical and financial problems in cDNA microarray. We had established the molecular diagnostic system for the prognosis of colorectal cancer with 30K human whole gene DNA chip using 200 surgical resected specimens, and we also identified the key genes related to the metastasis and recurrence.
With the results provided, we had revealed following two important knowledge related to gene expression profile of colorectal cancer; there is smaller difference of inter-tumor genetic heterogeneity than that of intra-tumor genetic heterogeneity, and so we can obtain representative gene expression profile using only one endoscopic biopsy. And we can distinguish colorectal adenoma and cancer from intact colorectal mucosa with our diagnostic system.
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