Molecular Diagnosis of colorectal cancer using preoperative endoscopic biopsies with clinical DNA chip

• Project/Area Number: 17591396
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Osaka University
• Principal Investigator: TAKEMASA Ichiro Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (50379252)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Hirofumi Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (30322184) ; IKEDA Masataka 0saka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (80335356) ; SEKIMOTO Mitsugu Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (10273658)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: colorectal cancer / DNA chip / gene expression profile / molecular diagnostic system / endoscopic biopsy / 遣伝子診断システム

Research Abstract

The aim of this study is to establish the preoperative molecular diagnostic system for colorectal cancer using endoscopic biopsies and to apply the system into clinic with the improvement of technical and financial problems in cDNA microarray. We had established the molecular diagnostic system for the prognosis of colorectal cancer with 30K human whole gene DNA chip using 200 surgical resected specimens, and we also identified the key genes related to the metastasis and recurrence.
With the results provided, we had revealed following two important knowledge related to gene expression profile of colorectal cancer; there is smaller difference of inter-tumor genetic heterogeneity than that of intra-tumor genetic heterogeneity, and so we can obtain representative gene expression profile using only one endoscopic biopsy. And we can distinguish colorectal adenoma and cancer from intact colorectal mucosa with our diagnostic system.

Research Products

• [Journal Article] Hypoxia-induced up-regulation of angiopoietin-2 in colorectal cancer. (2006)
  • Author(s): Gu J., et al.
  • Journal Title: Oncol Rep 15(4) Pages: 779-783
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma : a new approach to identify novel tumor markers. (2006)
  • Author(s): Kurokawa Y., et al.
  • Journal Title: Int J Oncol 28(2) Pages: 383-391
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Construction of a novel DNA decoy that inhibits the oncogenic beta-catenin/T-cell factor pathway. (2006)
  • Author(s): Seki Y., et al.
  • Journal Title: Mol Cancer Ther 5(4) Pages: 985-994
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Antisense to cyclin D1 inhibits, vascular endothelial growth factor-stimulated growth of vascular endothelial cells : implication of tumor vascularization. (2006)
  • Author(s): Yasui M., et al.
  • Journal Title: Clin Cancer Res 12(15) Pages: 4720-4729
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prediction of peritoneal metastasis in advanced gastric cancer by gene expression profiling of the primary site. (2006)
  • Author(s): Motoori M., et al.
  • Journal Title: Eur J Cancer 42 Pages: 1897-1903
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression and Mutation of SMAD4 in poorly Differentiated Carcinoma and Signet-ring Cell Carcinoma of the Colorectum. (2006)
  • Author(s): Seshimo I., et al.
  • Journal Title: J. Exp. Clin. Cancer Res 25 Pages: 433-442
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Low-dose oxaliplatin enhances the antitumor efficacy of paclitaxel in human gastric cancer cell lines. (2006)
  • Author(s): Gu J., et al.
  • Journal Title: Digestion 74(1) Pages: 19-27
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of PPARdelta in multistage carcinogenesis of the colorectum : implications of malignant cancer morphology. (2006)
  • Author(s): Takayama O., et al.
  • Journal Title: Br J Cancer 95(7) Pages: 889-895
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Correlation of GLUT-1 Overexpression, Tumor Size, and Depth of Invasion with 18F-2-fluoro-2-deoxy-D : -glucose Uptake by Positron Emission Tomography in Colorectal Cancer. (2006)
  • Author(s): Gu J., et al.
  • Journal Title: Dig Dis Sci 11(3) Pages: 2198-2205
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Involvement of ribonucleotide reductase M1 subunit overexpression in gemcitabine resistance of human pancreatic cancer. (2006)
  • Author(s): Nakahira S., et al.
  • Journal Title: Int J Cancer 27 120(6) Pages: 1355-1363
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Hypoxia-induced up-regulation of angiopoietin-2 in colorectal cancer. (2006)
  • Author(s): Gu J., Yamamoto H., Ogawa M., Ngan C.Y., Danno K., Hemmi H., Kyo N., Takemasa I., Ikeda M., Sekimoto M., Monden M.
  • Journal Title: Oncol Rep. 15(4) Pages: 779-783
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma : a new approach to identify novel tumor markers. (2006)
  • Author(s): Kurokawa Y., Honma K., Takemasa I., Nakamori S., Kita-Matsuo H., Motoori M, Nagano H, Dono K, Ochiya T., Monden M, Kato K.
  • Journal Title: Int J Oncol. 28(2) Pages: 383-391
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Construction of a novel DNA decoy that inhibits the oncogenic beta-catenin/T-cell factor pathway. (2006)
  • Author(s): Seki Y, Yamamoto H, Yee Ngan C, Yasui M, Tomita N, Kitani K, Takemasa I, Ikeda M, Sekimoto M, Matsuura N, Albanese C, Kaneda Y, Pestell RG, Monden M.
  • Journal Title: Mol Cancer Ther. 5(4) Pages: 985-994
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Antisense to cyclin DI inhibits vascular endothelial growth factor-stimulated growth of vascular endothelial cells : implication of tumor vascularization. (2006)
  • Author(s): Yasui M, Yamamoto H., Ngan CY., Damdinsuren B., Sugita Y., Fukunaga H., Gu J., Maeda M., Takemasa I., Ikeda M., Fujio Y, Sekimoto M., Matsuura N., Weinstein IB., Monden M.
  • Journal Title: Clin Cancer Res. 12(15) Pages: 4720-4729
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prediction of peritoneal metastasis in advanced gastric cancer by gene expression profiling of the primary site. (2006)
  • Author(s): Motoori M., Takemasa I., Doki Y., Saito S., Miyata H., Takiguchi S., Fujiwara Y, Yasuda T., Yano M., Kurokawa Y., Komori T., Yamasaki M., Ueno N., Oba S., Ishii S., Monden M., Kato K.
  • Journal Title: Eur J Cancer 42 Pages: 1897-1903
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression and Mutation of SMAD4 in poorly Differentiated Carcinoma and Signet-ring Cell Carcinoma of the Colorectum. (2006)
  • Author(s): Seshimo I, Yamamoto H., Mishima H., Kurata A., Suzuki R., Ezumi K., Takemasa 1., Ikeda M., Fukushima T., Tujinaka T., Sekimoto M., Kikkawa N., Takenoshita S., Monden M.
  • Journal Title: J.Exp.Clin.Cancer Res 25 Pages: 433-442
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Low-dose oxaliplatin enhances the antitumor efficacy of paclitaxel in human gastric cancer cell lines. (2006)
  • Author(s): Gu J., Yamamoto H., Lu X., Ngan CY., Tsujino T., Konishi K., Takemasa I., Ikeda M., Nagata H., Hashimoto S., Matsuzaki T., Sekimoto M., Takagi A., Monden M.
  • Journal Title: Digestion. 74(1) Pages: 19-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of PPARdelta in multistage carcinogenesis of the colorectum : implications of malignant cancer morphology. (2006)
  • Author(s): Takayama 0., Yamamoto H., Damdinsuren B., Sugita Y., Ngan CY., Xu X., Tsujino T., Takemasa I., Ikeda M., Sekimoto M., Matsuura N., Monden M.
  • Journal Title: Br J Cancer 95(7) Pages: 889-895
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Correlation of GLUT-1 Overexpression, Tumor Size, and Depth of Invasion with 18F-2-fluoro-2-deoxy-D : -glucose Uptake by Positron Emission Tomography in Colorectal Cancer. (2006)
  • Author(s): Gu J., Yamamoto H, Fukunaga H, Danno K, Takemasa I, Ikeda M, Tatsumi M, Sekimoto M, Hatazawa J, Nishimura T, Monden M.
  • Journal Title: Dig Dis Sci. 11(3) Pages: 2198-205
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvement of ribonuclectide reductase M1 Subunit overexpression in gemcttabine resistance of human pancreatic cancer. (2006)
  • Author(s): Nakahira S., et al.
  • Journal Title: Int J Canet 27 120(6) Pages: 1355-63
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hypoxia-induced up-regulation of angiopoietin-2 in colorectal cancer. (2006)
  • Author(s): Gu J., Yamamoto H., Ogawa M., Ngan C.Y., Danno K., Hemmi H., Kyo N., Takemasa I., Ikeda M., Sekimoto M., Monden M.
  • Journal Title: Oncol Rep 15(4) Pages: 779-783
• [Journal Article] Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma : a new approach to identify novel tumor markers. (2006)
  • Author(s): Kurokawa Y., Honma K., Takemasa I., Nakamori S., Kita-Matsuo H., Motoori M, Nagano H, Dono K, Ochiya T., Monden M, Kato K.
  • Journal Title: Int J Oncol 28(2) Pages: 383-391
• [Journal Article] Construction of a novel DNA decoy that inhibits the oncogenic beta-catenin/T-cell factor pathway. (2006)
  • Author(s): Seki Y, Yamamoto H, Yee Ngan C, Yasui M, Tomita N, Kitani K, Takemasa I, Ikeda M, Sekimoto M, Matsuura N, Albanese C, Kaneda Y, Pestell RG, Monden M.
  • Journal Title: Mol Cancer Ther 5(4) Pages: 985-994
• [Journal Article] Antisense to cyclin D1 inhibits vascular endothelial growth factor-stimulated growth of vascular endothelial cells : implication of tumor vascularization. (2006)
  • Author(s): Yasui M, Yamamoto H., Ngan CY., Damdinsuren B., Sugita Y., Fukunaga H., Gu J., Maeda M., Takemasa I., Ikeda M., Fujio Y., Sekimoto M., Matsuura N., Weinstein IB., Monden M.
  • Journal Title: Clin Cancer Res 12(15) Pages: 4720-4729
• [Journal Article] Prediction of peritoneal metastasis in advanced gastric cancer by gene expression profiling of the primary site. (2006)
  • Author(s): Motoori M., Takemasa I., Doki Y., Saito S., Miyata H., Takiguchi S., Fujiwara Y., Yasuda T., Yano M., Kurokawa Y., Komori T., Yamasaki M., Ueno N., Oba S., Ishii S., Monden M., Kato K.
  • Journal Title: Eur J Cancer 42 Pages: 1897-1903
• [Journal Article] Expression and Mutation of SMAD4 in poorly Differentiated Carcinoma and Signet-ring Cell Carcinoma of the Colorectum. (2006)
  • Author(s): Seshimo I, Yamamoto H., Mishima H., Kurata A., Suzuki R., Ezumi K., Takemasa I., Ikeda M., Fukushima T., Tujinaka T., Sekimoto M., Kikkawa N., Takenoshita S., Monden M
  • Journal Title: J. Exp. Clin. Cancer Res 25 Pages: 433-442
• [Journal Article] Low-dose oxaliplatin enhances the antitumor efficacy of paclitaxel in human gastric cancer cell lines. (2006)
  • Author(s): Gu J., Yamamoto H., Lu X., Ngan CY., Tsujino T., Konishi K., Takemasa I., Ikeda M., Nagata H., Hashimoto S., Matsuzaki T., Sekimoto M., Takagi A., Monden M.
  • Journal Title: Digestion 74(1) Pages: 19-27
• [Journal Article] Expression of PPARdelta in multistage carcinogenesis of the colorectum : implications of malignant cancer morphology. (2006)
  • Author(s): Takayama O., Yamamoto H., Damdinsuren B., Sugita Y., Ngan CY., Xu X., Tsujino T., Takemasa I, Ikeda M., Sekimoto M., Matsuura N., Monden M.
  • Journal Title: Br J Cancer 95(7) Pages: 889-895
• [Journal Article] Correlation of GLUT-1 Overexpression, Tumor Size, and Depth of Invasion with 18F-2-fluoro-2-deoxy-D : -glucose Uptake by Positron Emission Tomography in Colorectal Cancer. (2006)
  • Author(s): Gu J., Yamamoto H, Fukunaga H, Danno K, Takemasa I, Ikeda M, Tatsumi M, Sekimoto M, Hatazawa J, Nishimura T, Monden M.
  • Journal Title: Dig Dis Sci 11(3) Pages: 2198-2205
• [Journal Article] Involvement of ribonucleotide reductase M1 subunit overexpression in gemcitabine resistance of human pancreatic cancer. (2006)
  • Author(s): Nakahira S, Nakamori S., Tsujie M., Takahashi Y., Okami J., Yoshioka S., Yamasaki M., Marubashi S., Takemasa I., Miyamoto A., Takeda Y., Nagano H., Dono K., Umeshita K., Sakon M., Monden M.
  • Journal Title: Int J Cancer 27 120(6) Pages: 1355-1363
• [Journal Article] Central genetic alterations common to all HCV-positive, HBV-positive and non-B, non-C hepatocellular carcinoma : a new approach to identify novel tumor markers. (2006)
  • Author(s): Kurokawa Y, Honma K, Takemasa I, Nakamori S, Kita-Matsuo H, Motoori M, Nagano H, Dono K, Ochiya T, Monden M, Kato K.
  • Journal Title: Int J Oncnl. 28(2) Pages: 383-391
• [Journal Article] Prediction of recurrence in advanced gastric cancer patients after curative resection by gene expression profiling. (2005)
  • Author(s): Motoori M, Takemasa I, Yano M, Saito S, Miyata H, Takiguchi S, Fujiwara Y, Yasuda T, Doki Y, Kurokawa Y, Ueno N, Oba S, Ishii S, Monden M, Kato K.
  • Journal Title: Int J Cancer 114(6) Pages: 963-968
• [Journal Article] DNAマイクロアレイを用いたトランスクリプトーム解析による腫瘍マーカーの探索 (2005)
  • Author(s): 小森孝通, 竹政伊知朗, 山崎誠, 本告正明, 吉岡慎一, 小山太一, 池田正孝, 山本浩文, 関本貢嗣, (松原謙一), 門田守人
  • Journal Title: 日本分子腫瘍マーカー研究会誌 20 Pages: 76-77
  • NAID: 130004436355
• [Journal Article] 肝細胞癌・化学療法感受性予測-IFN/5FU併用療法の効果予測- (2005)
  • Author(s): 黒川幸典, 永野浩昭, 門田守人
  • Journal Title: 肝臓 46・10 Pages: 35-39
• [Book] 『消化器病学の進歩2005-モノグラフ 消化器病学のニューフロンティア編』DNA chipの癌臨床への応用-大腸癌診断用DNA chip(Mini Chip)の開発・作製-(荒川泰行 編集) (2005)
  • Author(s): 山崎誠, 竹政伊知朗, 門出守人
  • Total Pages: 407
  • Publisher: 財団法人日本消化器病学会
• [Book] 『癌転移のメカニズムがよくわかる 肺転移のすべて』2.網羅的遺伝子・タンパク解析 1)DNAマイクロアレイ (2005)
  • Author(s): 竹政伊知朗, 池田正孝, 山本浩文, 関本貢嗣, 門田守人
  • Total Pages: 146
  • Publisher: 永井書店