Identifying novel molecular markers of human hepatocellular carcinoma
| Project/Area Number |
17591397
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
TAKEDA Yutaka Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (90397696)
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| Co-Investigator(Kenkyū-buntansha) |
DONO Keizo Osaka University, Graduate School of Medicine, lecturer, 医学系研究科, 講師 (60283769)
NAGANO Hiroaki Osaka University, Graduate School of Medicine, lecturer, 医学系研究科, 講師 (10294050)
MIYAMOTO Atsushi Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (00362731)
MARUBASHI Shigeru Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (20362725)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | novel molecular markers of human hepatocellular carcinoma / DNA microarray analysis |
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| Research Abstract |
Samples from 100 HCC tissues and 7 normal livers without virus infection were obtained with informed consent from patients who underwent hepatic resection at Osaka University Hospital from 1997 to 2003. Gene expression profiling of primary HCC tumors from 100 patients was examined by human 30K DNA microarray(AceGene Human 30K; DNA Chip Research Inc and Hitachi Software Engineering Co., Yokohama Japan). The gene expression data were uploaded into the network analysis tool (Ingenuity systems, Mountain View, CA; http://www.ingenuity.com). We identified several activated "hotspot" regions containing a concentration of upregulated genes, and further focused on several of these genes. Several "hotspot" regions revealed integrin and Akt/NF-□B signaling as activated pathways in HCC. We identified key members linked to these signaling pathways including osteopontin (SPP1), glypican-3 (GPC3), vimentin (VIM), and more two genes. We compared gene expression levels generated from quantitative RT-PCR with those from microarray analysis by Pearson's correlation coefficients for each candidate gene. Each of the five genes analyzed showed significant correlation and confirmed the results obtained by DNA microarray. Immunohistochemical staining revealed colocalization of these genes in 15 (75%) of 20 human HCC samples. We discovered several molecules commonly upregulated in HCC as potential key players in this disease.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] TGF-betal-induced cell growth arrest and partial differentiation is related to the suppression of Id1 in human hepatoma cells.2006
Author(s)
Damdinsuren B., Nagano H., Kondo M., Natsag J., Hanada H., Nakamura M., Wada H., Kato H., Marubashi S., Miyamoto A., Takeda Y., Umeshita K., Dono K., Monden M.
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Journal Title
Oncol Rep 15(2)
Pages: 401-408
Related Report
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[Journal Article] Expression pattern of angiogenic factors and prognosis after hepatic resection in hepatocellular carcinoma : importance of angiopoietin-2 and hypoxia-induced factor-1 alpha.2006
Author(s)
Wada H., Nagano H., Yamamoto H., Yang Y., Kondo M., Ota H., Nakamura M., Yoshioka S., Kato H., Damdinsuren B., Tang D., Marubashi S., Miyamoto A., Takeda Y., Umeshita K., Nakamori S., Sakon M., Dono K., Wakasa K., Monden M.
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Journal Title
Liver International 26
Pages: 414-423
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[Journal Article] A case of hepatocellular carcinoma with multiple lung, spleen, and remnant liver metastasis successfully treated by combination chmotherapy with the novel oral DPD- inhibiting chemotherapeutic drug S-1 and interferon-α.2006
Author(s)
Nakamura M., Nagano H., Wada H., Noda T., Ota H., Damdinsuren B., Marubashi S., Miyamoto A., Takeda Y., Umeshita k., Dono K., Monden M.
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Journal Title
J Gastroenterol 10(1007)
Pages: 1120-1125
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[Journal Article] Vitamin K2 has growth inhibition effect against hepatocellular carcinoma cell lines but does not enhance anti-tumor effect of combination treatment of interferon-alpha and fluorouracil in vitro.2006
Author(s)
Nakamura M, Nagano H, Noda T, Wada H, Ota H, Damdinsuren B, Marubashi S, Miyamoto A, Takeda Y, Doki Y, Umeshita K, Dono K, Sakon M, Monden M.
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Journal Title
Hepatology Research 35
Pages: 289-295
Related Report
