| Project/Area Number |
17591397
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
TAKEDA Yutaka Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (90397696)
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| Co-Investigator(Kenkyū-buntansha) |
DONO Keizo Osaka University, Graduate School of Medicine, lecturer, 医学系研究科, 講師 (60283769)
NAGANO Hiroaki Osaka University, Graduate School of Medicine, lecturer, 医学系研究科, 講師 (10294050)
MIYAMOTO Atsushi Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (00362731)
MARUBASHI Shigeru Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (20362725)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | novel molecular markers of human hepatocellular carcinoma / DNA microarray analysis |
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| Research Abstract |
Samples from 100 HCC tissues and 7 normal livers without virus infection were obtained with informed consent from patients who underwent hepatic resection at Osaka University Hospital from 1997 to 2003. Gene expression profiling of primary HCC tumors from 100 patients was examined by human 30K DNA microarray(AceGene Human 30K; DNA Chip Research Inc and Hitachi Software Engineering Co., Yokohama Japan). The gene expression data were uploaded into the network analysis tool (Ingenuity systems, Mountain View, CA; http://www.ingenuity.com). We identified several activated "hotspot" regions containing a concentration of upregulated genes, and further focused on several of these genes. Several "hotspot" regions revealed integrin and Akt/NF-□B signaling as activated pathways in HCC. We identified key members linked to these signaling pathways including osteopontin (SPP1), glypican-3 (GPC3), vimentin (VIM), and more two genes. We compared gene expression levels generated from quantitative RT-PCR with those from microarray analysis by Pearson's correlation coefficients for each candidate gene. Each of the five genes analyzed showed significant correlation and confirmed the results obtained by DNA microarray. Immunohistochemical staining revealed colocalization of these genes in 15 (75%) of 20 human HCC samples. We discovered several molecules commonly upregulated in HCC as potential key players in this disease.
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