| Project/Area Number |
17591406
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
IIZUKA Norio Yamaguchi University, Graduate School of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (80332807)
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| Co-Investigator(Kenkyū-buntansha) |
TAMESA Takao Yamaguchi University, Hospital, Assistant Professor, 医学部附属病院, 助手 (30359905)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | ID2 / Hepatocellular carcinoma / metastasis / HCC / portal vein invasion |
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| Research Abstract |
The purpose of this study is to explore the molecular and biologic functions of Inhibitor of DNA binding/differentiation 2 (ID2), which was found to be responsible for portal vein invasion (PVI) of hepatocellular carcinoma (HCC) in a previous genome-wide search for the PVI-related genes.
(1) To validate the expression pattern on our previous DNA microarray study, we measured ID2 mRNA levels in a new cohort of 92 HCCs by real-time reverse transcription-PCR. ID2 mRNA level correlated inversely with PVI (P < 0.001), TNM stage (P < 0.001), tumor size (P < 0.001), and early intrahepatic recurrence (P < 0.05). Patients with low levels of ID2 had significantly poorer prognoses than those with high levels of ID2.
(2)To clarify the precise roles of ID2, we performed in vitro analysis with expression vectors and small interfering RNAs. These gene-targeting experiments revealed inhibitory effects of ID2 on the invasive potential of HCC cells, which could be explained in part by altering levels and/or secretion of MMPs and some angiogenesis-related proteins.
We reported the relation between ID2 mRNA levels and progression of HCC (Tsunedomi et al., Int J Oncol, 2006). Moreover, we are submitting a paper in combination with the above results ((1) and (2)). Also, the relevant findings on HCC research were reported in 5 scientific medical journals worldwide.
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