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Development of pancreatic cancer therapy and prevention using morphogen as a target signaling pathway

Research Project

Project/Area Number 17591414
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YAMAGUCHI Kouji  Kyushu University, Faculty of Medical Sciences, Associate Professor, 大学院医学研究院, 助教授 (50191226)

Co-Investigator(Kenkyū-buntansha) KATANO Mitsuo  Kyushu University, Faculty of Medical Sciences, Professor, 大学院医学研究院, 教授 (10145203)
NOMURA Masatoshi  Kyushu University, Faculty of Medical Sciences, Research Associate, 大学病院, 助手 (30315080)
NOMURA Masafumi  Kyushu University, Faculty of Medical Sciences, Associate Professor, 大学院医学研究院, 助教授 (30372741)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsIL1-β / pancreatic cancer / Hedgehog / NFkB / inflammation / obstructive pancreat it is / Sonic Hedgehog / invasion
Research Abstract

It has been reported that Hedgehog (HH) signal activation of ligand (Shh) dependence participates in pancreas carcinogenesis and development, but Shh is not clarified about mechanism developing excessively in any cancers including pancreatic cancer. In this theme, we analyzed the correlation between activity of nuclear factor-KB (NF-κB) and expression level of Shh in pancreatic cancer, because NF-κB is usually activated by obstructive pancreatitis caused by pancreatic cancer. We found a positive correlation between NF-κB p65 and Shh expression in surgically resected pancreas specimens, including specimens of chronic pancreatitis and pancreatic adenocarcinoma. Suppression of NF-κB by PDTC, decoy ODN or dominant-negativeNF-κB suppressed constitutive expression of Shh mRNA in pancreatic cancer cells. Vice versa, three inflammatory stimuli including IL1-β, TNF-a and LPS induced overexpression of Shh, resulting in activation of the Hh pathway, consistent with that blockade of NF-κB suppressed overexpression of Shh induced by these stimuli. Importantly, NF-KB-induced HH signal activation enhanced cell proliferation in pancreatic cancer cells. In addition, inhibition of the Hh pathway as well as NF-κB suppressed the enhanced cell invasion. Our data suggest that NF-κB activation is one of the mechanisms controling Shh overexpression in pancreatic cancer and that proliferation and invasion of pancreatic cancer cells is accelerated by NF-κB activation in part through HH signaling pathway.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (4 results)

All 2006 2005 Other

All Journal Article (4 results)

  • [Journal Article] Nuclear Factor-KB Contributes to Hedgehog Signaling Pathway Activation through Sonic Hedgehog Induction in Pancreatic Cancer2006

    • Author(s)
      Nakashima H., et al.
    • Journal Title

      Cancer Research 66: (14)

      Pages: 7041-7049

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nuclear Factor-KB Contributes to Hedgehog Signaling Pathway Activation through Sonic Hedgehog Induction in Pancreatic Cancer2006

    • Author(s)
      Nakashima H., et al.
    • Journal Title

      Cancer Research 66:(14)

      Pages: 7041-7049

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Immunohistochemical staining of hedgehog pathway-related proteins in human thymomas.2005

    • Author(s)
      Tasaki A., et al.
    • Journal Title

      Anticancer Res. 25(6A)

      Pages: 3697-3701

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Gli1, down-regulated in colorectal cancers, inhibits proliferation of colon cancer cells involving Wnt signalling activation.

    • Author(s)
      Akiyoshi T, et al.
    • Journal Title

      GUT in press

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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