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Establishment of the hepatic reserve and molecular biological analyses of liver function after hepatectomy.

Research Project

Project/Area Number 17591417
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionUniversity of Miyazaki

Principal Investigator

CHIJIIWA Kazuo  University of Miyazaki, Surgery 1, Professor (90179945)

Co-Investigator(Kenkyū-buntansha) KAI Masahiro  Miyazaki University, Surgery 1, Assistant Professor (40264379)
HIDAKA Hideki  Miyazaki University, Surgery 1, Assistant Professor (90398264)
MAEHARA Naoki  Miyazaki University, Surgery 1, Assistant Professor (30381065)
OHUCHIDA Jiro  Miyazaki University, Surgery 1, Assistant Professor (10363590)
OTANI Kazuhiro  Miyazaki University, Surgery 1, Assistant Professor (60437936)
旭吉 雅秀  宮崎大学, 医学部, 医員
自見 政一郎  宮崎大学, 医学部, 助手 (90363598)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsobstructive jaundice / hepatectomy / liver failure / bile acid transporter / portal vein ligation / 閉塞性黄疽
Research Abstract

1. The differences in the expression of the organic anion (bile acid, bilirubin) transporters depending on the duration of bile duct ligation have been examined in rats. Blood and liver tissues during the period from 3 to 28 days after the ligation were obtained. The excretion transporters, the mRNA expressions of mrp2 decreased while those of mrp3 increased from 3 days until 28 days after the ligation. The uptake transporters, ntcp and oatp decreased until 28 days after the ligation, while the mdr2 did not change after the ligation. After biliary drainage, the change in expression of these mRNA and protein levels are now examined.
2. The differences in hepatic reserve function and parameters to predict the liver failure in the early stage after extended hepatectomy were examined in rats. We determined the bile flow, biliary bile acid secretion and the mRNA expressions of bile acid transporters after 70 % or 90 % hepatectomy in rats. The bile flow and bile acid secretion both decreased … More significantly after the 90 % hepatectomy, which is a model for acute liver failure (ref.3). Biliary bile acid excretion and the expression of mrp2 decreased, while the expressions of mrp3 and bsep increased after 90 % hepatectomy. In contrast, the expression of mrp2 increased, while the expressions of mrp3 and bsep decreased after 70 % hepatectomy. The expressions of mrp2 and mrp3 was differently regulated after 70 % and 90 % hepatectomy. We suspected that expressions of mrp2 and mrp3 might be able to predict the liver failure after extended hepatectomy.
3. We examined the expression of growth factors, transcription factors, and cell cycle regulators in response to regeneration after a two-third hepatectomy and portal vein branch ligation. We reported that the mRNA expression of Cyclin Dl decreased in the atrophying and increased in the proliferating lobes after portal vein branch ligation and after hepatectomy in rats. We also reported the mechanisms of liver damage after hepatic ischemia and reperfusion in rats (ref.4). Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (31 results)

All 2007 2006 2005 Other

All Journal Article (21 results) (of which Peer Reviewed: 9 results) Presentation (10 results)

  • [Journal Article] Outcome of Radical Surgery for Stage IV Gallbladder Carcinoma2007

    • Author(s)
      Chijiiwa, K., Kai, M., Nagano, M., et. al.
    • Journal Title

      Journal of Hepato-Biliary-Pancreatic Surgery 14

      Pages: 345-350

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A Curative Resection Improves the Postoperative Survival Rate Even in Patients with Advanced Gallbladder Carcinoma2007

    • Author(s)
      Kai, M., Chijiiwa, K., Ohuchida, J., et. al.
    • Journal Title

      Journal of Gastrointestinal Surgery 11

      Pages: 1025-1032

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Outcome of Radical Surgery for Stage IV Gallbladder Carcinoma2007

    • Author(s)
      Chijiiwa, K., Kai, M., Nagano, et. al.
    • Journal Title

      Journal of Hepato-Biliary-Pancreatic Surgery 14

      Pages: 345-350

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Vertical Retrocolic Duodenojejunostomy Decreases Delayed Gastric Emptying after Pylorus Preserving Pancreatoduodenectomy.2007

    • Author(s)
      Chijiiwa, K., Ohuchida, J., Hiyoshi, M., et. al.
    • Journal Title

      Hepato-Gastroenterology 51・3

      Pages: 1874-1877

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Outcome of Radical Surgery for StageIV Gallbladder Carcinoma.2007

    • Author(s)
      Chijiiwa, K., Kai, M., Nagano, M., et. al.
    • Journal Title

      Journal of Hepato-Biliary-Pancreatic Surgery 14・4

      Pages: 345-350

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Curative Resection Improve the Postoperative Surgical Rate Even in Patients with Advanced Gallbladder Carcinoma.2007

    • Author(s)
      Kai, M., Chijiiwa, K., Ohuchida, J., et. al.
    • Journal Title

      Journal of Gastrointestinal Surgery 11・8

      Pages: 1025-1032

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 膵胆管合流異常における胆嚢粘膜上皮細胞のDNA酸化障害.2007

    • Author(s)
      大谷和広, 千々岩一男:
    • Journal Title

      胆と膵 28・12

      Pages: 1029-1034

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 胆嚢癌に対するS_4a+S_5切除のエビデンス.2007

    • Author(s)
      甲斐真弘, 千々岩一男:
    • Journal Title

      外科 69・11

      Pages: 1282-1290

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Biliary Lipid Output in the Early Stage of Acute Liver Failure Induced by 90%Hepatectomy in the Rat2006

    • Author(s)
      Tanaka, S.-I., Chijiiwa, K., Maeda, Y.
    • Journal Title

      Joumal of Surrgical Research 134

      Pages: 81-86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Inhibition of the Nitric Oxide Synthase Prevents Hepatic, but Not Pulmonary, Injury Following Ischemia-Reperfusion of Rats2006

    • Author(s)
      Takamatsu, Y., Shimada, K., Yamaguchi, K., et. al.
    • Journal Title

      Digestive Diseases and Sciences 51

      Pages: 571-579

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Biliary Lipid Output in the Early Stage of Acute Liver Failure Induced by 90% Hepatectomy in the Rat2006

    • Author(s)
      Tanaka, S.-I., Chiiiiwa, K., Maeda, Y
    • Journal Title

      Journal of Surgical Research 134

      Pages: 81-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of Inducible Nitric Oxide Synthase Prevents Hepatic, but Not Pulmonary, Injury Following Ischemia-Reperfusion of Rat Liver2006

    • Author(s)
      Takamatsu, Y., Shimada, K., Yamagichi, K., et. al.
    • Journal Title

      Digestive Diseases and Sciences 51

      Pages: 571-579

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of Inducible Nitric Oxide Synthase Prevents Hepatic, but not Pulmonary, Injury Following Ischemia-Reperfusion of Rat Liver.2006

    • Author(s)
      Takamatsu, S., Shimada, K., Yamaguchi, K., et al.
    • Journal Title

      Digestive Diseases and Sciences 51・3

      Pages: 571-579

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Biliary Lipid Output in the Early Stage of Acute Liver Failure Induces by 90% Hepatectomy in the Rat.2006

    • Author(s)
      Tanaka, S-I., Chijiiwa, K., Maeda, Y.
    • Journal Title

      Journal of Surgical Research 134・1

      Pages: 81-86

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Hs肝細胞癌に対する選択的肝阻血法を用いた系統的肝亜区域切除術による長期予後の改善2005

    • Author(s)
      牧野 一郎, 千々岩 一男, 近藤 千博, 永野 元章, 旭吉 雅秀, 大内田 次郎
    • Journal Title

      日本消化器外科学会雑誌 38

      Pages: 1407-1413

    • NAID

      110001789812

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Prognostic Benefit of Selective Portal Vein Occlusion During Hepatic Resection for Hepatocellular Carcionoma2005

    • Author(s)
      Makino, I., Chijiiwa, K., Kondo, K., et. al.
    • Journal Title

      Surgery 137

      Pages: 626-631

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 胆嚢癌手術における拡大リンパ節郭清とその適応2005

    • Author(s)
      千々岩 一男, 甲斐 真弘, 大内田 次郎, 旭吉 雅秀, 永野 元章, 前原 直樹, 近藤 千博
    • Journal Title

      消化器外科 28

      Pages: 1499-1505

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effective of Anatomical Subsegmentectomy of the Liver Under Selective Portal Inflow Occlusion in Patients with Hepatocellular Carcinoma Limited to a Subsegment2005

    • Author(s)
      Makino, I., Chijiiwa, K., Kondo, K., et. al.
    • Journal Title

      The Japanese Journal of Gastroenterological Surgery 38

      Pages: 1407-1413

    • NAID

      110001789812

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Prognostic Benefit of Selective Portal Vein Occlusion During Hepatic Resection for Hepatocellular Carcinoma2005

    • Author(s)
      Makino, I., Chijiiwa, K., Ohuchida, J., et. al.
    • Journal Title

      Surgery 137

      Pages: 626-631

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Significance and Indication of Extended Lymph Node Dissecction for Advanced Gallbladder Carcinoma2005

    • Author(s)
      Chijiiwa, K., Kai, M., Ohuchida, et. al.
    • Journal Title

      Gastroenterological Surgery 28

      Pages: 1499-1505

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Biliary Lipid Output in the Early Stage of Acute Liver Failure Induced by 90% Hepatectomy in the Rat.

    • Author(s)
      Tanaka, S-I., Chijiiwa, K., Maeda, Y.
    • Journal Title

      Journal of Surgical Research (in press)

    • Related Report
      2005 Annual Research Report
  • [Presentation] ラット急性肝不全モデルにおける有機アニオントランスポーターの変動2007

    • Author(s)
      佐野 浩一郎, 千々岩 一男, 甲斐 真弘, 日高 淑晶, 金丸 幹郎
    • Organizer
      第107回日本タト科学会定期学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2007-04-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of Organic Anion Transporters in Acute Liver Failure Rat Model2007

    • Author(s)
      Sano, K., Chijiiwa, K., Kai, M., Hidaka, Y., Kanemaru, M.
    • Organizer
      The 108th Annual Congress of Japan Surgical Society
    • Place of Presentation
      Osaka
    • Year and Date
      2007-04-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 肝細胞増殖因子活性化因子(HGFA)knockoutマウスを用いた肝再生能の検討2006

    • Author(s)
      内山 周一郎, 長池 幸樹, 千々岩 一男, 片岡 寛章
    • Organizer
      第48回日本消化器病学会大会(DDW-Japan 2006)
    • Place of Presentation
      札幌
    • Year and Date
      2006-10-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Liver Regeneration after Hepatectomy in HGFA Knockout Mice2006

    • Author(s)
      Uchivama, S., Nasaike, K., Chiiiiwa, K, Kataoka, H
    • Organizer
      The 48th Annual Meeting of the Japanese Society of Gastroenterology
    • Place of Presentation
      Sapporo
    • Year and Date
      2006-10-13
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 胆汁酸が培養肝細胞の増殖能に及ぼす影響2006

    • Author(s)
      豊永 健二, 菊池 秀彦, 中山 建男, 千々岩 一男
    • Organizer
      第106回日本外科学会定期学術集会
    • Place of Presentation
      東京
    • Year and Date
      2006-03-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of Bile Acids on Proliferation of Cultured Rat Hepatocyte2006

    • Author(s)
      Toyonaga, T., Kikuchi, H., Nakayama, T., Chiiiiwa, K.
    • Organizer
      The 106th Annual Congress of Japanese Surgical Society
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-03-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 閉塞性黄疸および胆汁内,外による減黄瘻後のトランスポーターの発現変化2006

    • Author(s)
      日高 淑晶, 千々岩 一男, 自見 政一郎, 佐野 浩一郎, 金丸 幹郎, 内山 周一郎, 甲斐 真弘
    • Organizer
      第106回日本タト科学会定期学術集会
    • Place of Presentation
      東京
    • Year and Date
      2006-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression of Bile Acid Transporters after Internal or External Drainage in Obstructive Jaundiced Rat Model2006

    • Author(s)
      Hidaka, Y., Chijiiwa, K., Jimi, S.-I., et. al.
    • Organizer
      The 106th Annual Congress of Japanese Surgical Society
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 閉塞性黄疸および減黄後の肝臓のtransporterの変化2005

    • Author(s)
      日高 淑晶, 千々岩 一男, 自見 政一郎, 佐野 浩一郎, 内山 周一郎, 甲斐 真弘
    • Organizer
      第17回西日本臨床胆汁酸研究会
    • Place of Presentation
      奈良
    • Year and Date
      2005-07-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of Biliary Drainage on Expression of Organic Anion Transporters in Obstructive Jaundiced Rats2005

    • Author(s)
      Hidaka, Y., Chijiiwa, K., Jimi, S.-I., et. al.
    • Organizer
      The 17th Nishi-Nihon Clinical Bile Acid Meeting
    • Place of Presentation
      Nara
    • Year and Date
      2005-07-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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