| Project/Area Number |
17591428
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
KIKUCHI Shojiro Kyoto Prefectural University of Medicine, Graduate School of Medicine, assistant, 医学研究科, 助教(学内講師) (70381960)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OCHIAI Toshiya Kyoto Prefectural University of Medicine, Graduate School of Medicine, assistant, 医学研究科, 助教(学内講師) (30311913)
YAMAGISHI Hisakazu Kyoto Prefectural University of Medicine, Graduate School of Medicine, president, 医学研究科, 学長 (40128723)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2006: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | carcinoma / development / differentiation / cell / tissue / 分化 / サイトカイン |
|---|
| Research Abstract |
1. Clinical sample from cancer patient-Twenty samples of surgical specimens from esophageal cancer patient were successfully collected for RNA analysis and primary culture.
2. Dermokine expression assay---Real-time PCR assay show that expression levels of dermokine-beta were low in cancer tissue.
3. Primary culture of resected esophageal cancer tissue---We add recombinant dermokine-beta to cultured primary cell to clarify the function of dermokine-beta in squamous cell carcinoma cell.
4. Large scale purification of dermokine-alpha, beta---We successfully established 293EBNA-1 cell expression system to generate recombinant dermokine-beta protein. Large scale purification of dermokine-alpha were not successful.
5. Variant isoform of dermokine-We analyzed morphological change of esophageal cancer cell under various concentration of recombinant dermokine-beta, but we could not find obvious changes. Recently various isoform of dermokine were reported in other group. Now we focused on isoform specific function of dermokine in cancer cell.
|
