Study on pancreatic cell lineage of growth and differentiation in culture cells and tissue
| Project/Area Number |
17591443
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
TSUCHIYA Mariko Tokyo Women's Medical University, School of Medicine, Senior Lecturer (00266826)
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| Co-Investigator(Kenkyū-buntansha) |
TSUCHIYA Ken Tokyo Women's Medical University, School of Medicine, Associate Professor (00246472)
YASUDA Kazuki International Medical Center of Japan, 研究部, Division Manager (80311611)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,580,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | pancreatic cell / β cell / transcription factor / siRNA / adipocyte / adinocytokine / mafA / c-maf / mafB / insulin / transcription factor / インスリン / 発生 分化 / β細胞 / maf |
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| Research Abstract |
Maf is a family of transcription factor proteins characterized by a typical bZip structure, a motif for protein dimerization and DNA binding, have been reported to be regulating several distinct developmental processes, cellular differentiation and establishment of function. We have shown the expression profiles of the large maf family in the developing pancreas of human and porcine(Pancreas 2006). One of the large maf molecules, mafA, has been found to be as a strong transactivator of insulin gene transcription in pancreatic p cells, however, it is now clear that the maf family not only regulate islet development but the development of the the entire pancreas and adipose tissue, resulting in the establishment of the glucose and adipokine network. We reported that suppression of the mafA mRNA level in the mouse pancreas by the RNA interference technique down-regulated expression of the adipocytokine genes in addition to the genes encoding insulin and glucagons(BBRC 2007). Adipocytes are highly involved in insulin action and glucose metabolism as well as in lipid metabolism. To explore the role of mafA in adipocytes, we investigated mafA mRNA expression and modified its level in cultured adipocytes, 3T3-L1 cells, by the siRNA technique, and analyzed the resulting alterations of morphological changes and the gene profile related to adipogenesis and adipocytokines during the differentiation. Cell differentiation was attenuated by mafA suppression, and no adipodroplet accumulation was observed in the cytoplasm of the adipocytes. mRNA expression of adipogenic genes which are essential genes for adipocyte differentiation, was suppressed by mafA siRNA interference. Thus, mafA is a key modulator and has multi-potentiality for establishing cell differentiation and cell function, that are involved in glucose and lipid metabolism.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] In vivo suppression of mafA mRNA with siRNA and analysis of the resulting alteration of the gene expression profile in mouse pancreas by the microarray method2007
Author(s)
Mariko, Tsuchiya, Takumi, Yoshida, Shigeki, Taniguchi, Kazuki, Yasuda, Atsushi, Maeda, Akiko, Hayashi, Junko Tanaka, Mutsuo, Shigemoto, Kohsaku, Nitta, Ken, Tsuchiya
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Journal Title
Biochemical Biophysical Research Communications 356
Pages: 129-135
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Potential roles of large mafs in cell lineages and developing pancreas2006
Author(s)
Mariko, Tsuchiya, Shigeki, Taniguchi, Kazuki, Yasuda, Kosaku, Nitta, Atsushi, Maeda, Mutsuo, Shigemoto, Ken Tsuchiya
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Journal Title
Pancreas 32
Pages: 408-416
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Gene profile related to mafs in vivo by siRNA techinique and microarray analysis in physiological and pathophysiological metabolic responses2007
Author(s)
Mariko, Tsuchiya, Atsushi, Maeda, Akiko, Hayashi, Junko, Tanaka, Takumi, Yoshida, Shigeki, Taniguchi, Kazuki Yasuda, Kosaku, Nitta, Ken, Tsuchiya
Organizer
67th scientific sessions of American Diabetic Association
Place of Presentation
Chicago
Year and Date
2007-06-24
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] In vivo suppression of mafs mRNA using siRNA and resulting alteration of gene expression profile in mouse pancreas by microarray analysis2006
Author(s)
Mariko, Tsuchiya, Takumi, Yoshida, Shigeki, Taniguchi, Kazuki, Yasuda, Atsushi, Maeda, Mutsuo, Shigemoto, Ken Tsuchiya
Organizer
66th scientific sessions of American Diabetic Association
Place of Presentation
Washington
Description
「研究成果報告書概要(欧文)」より
Related Report
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