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The strategy based on gene expression profiling using microarray for tailor-made treatment in patients with lung cancer

Research Project

Project/Area Number 17591458
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionChiba University

Principal Investigator

IYODA Akira  Chiba University, Hospital, Assistant Professor, 医学部附属病院, 助手 (10302548)

Co-Investigator(Kenkyū-buntansha) FUJISAWA Takehiko  Chiba University, Graduate School of Medicine, Professor, 大学院医学研究院, 教授 (80110328)
SEKI Naohiko  Chiba University, Graduate School of Medicine, Associate Professor, 大学院医学研究院, 助教授 (50345013)
SHIBUYA Kiyoshi  Chiba University, Hospital, Associate Professor, 医学部附属病院, 講師 (20302565)
HIROSHIMA Kenzo  Chiba University, Graduate School of Medicine, Associate Professor, 大学院医学研究院, 助教授 (80218833)
MOTOHASHI Shinichiro  Chiba University, Graduate School of Medicine, Associate Professor, 大学院医学研究院, 産学官連携研究員 (60345022)
飯笹 俊彦  千葉大学, 大学院・医学研究院, 助教授 (10272303)
守屋 康充  千葉大学, 医学部附属病院, 医員 (90375692)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsLung cancer / Microarray / Prognosis / tailor-made treatment
Research Abstract

Purpose Lymph node metastasis and tumor recurrence are major factors associated with poor prognosis in the cancer, but little is known of their molecular mechanisms. The aim of this study was to identify genes differentially expressed between normal and cancerous lung tissues, and to investigate the gene-expression profiles of 100 primary lung cancers to select a set of gene predictors for the clinical features of lung cancer.
Experimental Design Gene expression profiles were obtained using an oligonucleotide microarray, and the construction of predictor sets was performed by evaluating the statistical significance of the expression levels of selected genes.
Results In the search for candidate genes, 530 genes showed differential expression in adenocarcinoma and 519 genes in squamous cell carcinoma. Ninety-four genes showed a distinct expression pattern exclusively in cancer tissues with lymph-node metastasis and 60 genes showed involvement with tumor recurrence. Using the most suitable set of genes, it was possible to predict the clinical futures of patients with lung cancer. The prediction scoring system yielded 90.9% accuracy for forecasting adenocarcinoma and 91.5% accuracy for squamous cell carcinoma, 71.4% accuracy for forecasting lymph node metastasis and 84.6% accuracy for tumor recurrence in independent cases.
Conclusions The gene expression analysis and the combination of statistical analysis successfully distinguished histopathological types and clinical features such as lymph node metastasis and recurrence. The findings of this study show the possibility of subgrouping lung cancer based on the combination of pathological diagnosis and molecular classification.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (9 results)

All 2007 2006 Other

All Journal Article (9 results)

  • [Journal Article] Cytological findings of pre-invasive bronchial lesions detected by light-induced fluorescence endoscopy in a lung cancer screening system2007

    • Author(s)
      Baba M, Iyoda A, et al.
    • Journal Title

      Oncol Rep 17

      Pages: 579-583

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Transbronchial fine needle aspiration cytological examination : A useful tool for diagnosing primary lung cancer2006

    • Author(s)
      Iyoda A, et al.
    • Journal Title

      Thoracic and Cardiovascular Surgeon 54

      Pages: 117-119

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Prognostic impact of large cell neuroendocrine histology in patients with pathological stage la pulmonary non-small cell carcinoma2006

    • Author(s)
      Iyoda A, et al.
    • Journal Title

      The Journal of Thoracic and Cardiovascular Surgery 132

      Pages: 312-315

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prospective study of adjuvant chemotherapy for pulmonary large cellneuroendocrine carcinoma2006

    • Author(s)
      Iyoda A, et al.
    • Journal Title

      Ann Thorac Surg 82

      Pages: 1802-1807

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 肺大細胞神経内分泌癌2006

    • Author(s)
      伊橡田明, 他
    • Journal Title

      肺癌 46

      Pages: 315-320

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prospective study of adjuvant chemotherapy for pulmonary large cell neuroendocrine carcinoma2006

    • Author(s)
      Iyoda A, et al.
    • Journal Title

      Ann Thorac Surg 82

      Pages: 1802-1807

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pulmonary large cell neuroendocrine carcinoma2006

    • Author(s)
      Iyoda A, et al.
    • Journal Title

      Japanese Journal of Lung Cancer 46

      Pages: 315-320

    • NAID

      110004813245

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 肺大細胞神経内分泌癌2006

    • Author(s)
      伊豫田明, 他
    • Journal Title

      肺癌 46

      Pages: 315-20

    • NAID

      110004813245

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Prognostic impact of large cell neuroendocrine histology in patients with pathological stage 1a pulmonary non-small cell carcinoma

    • Author(s)
      Iyoda A, et al.
    • Journal Title

      The Journal of Thoracic and Cardiovascular Surgery (In press)

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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