| Project/Area Number |
17591460
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAJIMA Jun The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (90188954)
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| Co-Investigator(Kenkyū-buntansha) |
KAKIMI Kazuhiro The University of Tokyo, Faculty of Medicine, Project Associate Professor, 医学部附属病院, 寄附講座教員(客員助教授) (80273358)
MURAKAWA Tomohiro The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (50359626)
FUKAMI Takeshi The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (40396742)
KURACHI Makoto The University of Tokyo, Graduate School of Medicine, Research Associate, 大学院医学系研究科, 助手 (00396722)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | non-small cell lung cancer / gamma / delta - T lymphocyte / immunotherapy / clinical trial / safety / recommended dosage / anticancer effect / NKG2D |
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| Research Abstract |
This study aimed to investigate the safety and antitumor effect of the new immunotherapy with activated autologous gamma delta T-lymphocyte (GDT) on the patients suffering from recurrent non-small cell lung cancer. Approximately 1000-folds increase of the number GDT, comprising 85 to 95% of whole cultured mononuclear cells, was observed. This GDT fraction expresses NKG2D, showing cytotoxic effect against MICA/B-positive non-small cell cancer (NSCLC) cell lines.
Under the admission of IRB in this hospital, we started the clinical trial on the treatment of refractory NSCLC with activated autologous GDT. We registered 8 patients with recurrent NSCLC after the documented informed consent. All except for 2 patients who attained enough number of GDT after in vitro culture were assigned to this clinical investigation.
We observed no harmful complications after administration of cultured GDT, except for one case who had suffered from common cold during the trial period, which were not related to the GDT therapy. We found that one of 3 patients who had undergone FACT-QOL questionnaire had exacerbation of QOL during the study, which were mainly due to the common cold.
All of the patients had measurable foci of recurrence, which were not decreased in size by Computed tomography during the study. We performed flow-cytometry study of the peripheral blood of the patients undergoing GDT therapy, and we observed that number of GDT was increased during the therapy. We also observed cytotoxic effect of the GDT against U266 cell line which expresses MICA on the surface. We confirmed that NKG2D-MICA/B cytotoxic effect on GDT of the patients.
In this study so far, we actually gave each patient the activated GDT only 4 times. We suggest that increment of the dosage of GDT would make breakthrough on the immunotherapy of NSCLC.
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