Regeneration therapy of coronaray artery conbined growth factor and omental flap as flow source

• Project/Area Number: 17591468
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: SAJI Yoshiaki Kyoto University, Graduate School of Medicine, Assistant professor, 医学研究科, 助手 (00399776)
• Co-Investigator(Kenkyū-buntansha): TABATA Yasuhiko Kyoto University, Department of Biomaterials Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (50211371) ; KOMEDA Masashi Kyoto University, Graduate School of Medicine, professor, 医学研究科, 教授 (20303810) ; MARUI Akira Kyoto University, Graduate School of Medicine, Assistant professor, 医学研究科, 助手 (60402856)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: Arteriogenesis / Biologic coronary artery grafting / Omental flap / Controlled-release bFGF / Bio-CABG / バイオバイパス

Research Abstract

OBJECTIVE : The purpose of this study was to evaluate the therapeutic efficacy of the combined growth factor therapy with an omental flap in a rabbit model of chronic myocardial ischemia.
METHODS : Chronic ischemia was created in rabbits by placing a constrictor on the left circumflex artery. Four weeks later the animals were divided into 3 groups: group FG, in which a gelatin hydrogel sheet incorporating 100 microg of basic fibroblast growth factor was placed over the left circumflex region followed by covering with the omental flap including the intact gastroepiploic artery ; group F, in which only the basic fibroblast growth factor sheet was placed; and group N, in which no treatment was done.
RESULTS : Cine magnetic resonance imaging analysis showed a greater percentage wall thickening in the left circumflex region in group FG than in other groups (group FG, 49.2% +/-4.5%; group F, 41.2% +/-3.8% ; group N, 32.1% +/-2.5%, P =.035, group FG vs group F). A colored microsphere assay showed higher perfusion in the left circumflex region in group FG than in group F. Perfusion in the left circumflex region was decreased after clamping the gastroepiploic artery pedicle in group FG (before clamping, 2.83 +/-0.72 mL × min(-1) × g(-1); after clamping, 1.93 +/-0.59 mL × min(-1) × g(-1); P <.01). In vivo angiography via gastroepiploic artery showed direct "to-and-fro" visible collaterals between the gastroepiploic and occluded left circumflex coronary arteries in group FG.
CONCLUSION : The combined growth factor therapy with an omental flap induced arteriogenesis and provided additional perfusion via the gastroepiploic artery to ameliorate regional dysfunction in the chronically ischemic myocardium.

Research Products

• [Journal Article] A combination of omental flap and growth factor therapy induces arteriogenesis and increases myocardial perfusion in chronic myocardial ischemia : evolving concept of biologic coronary artery bypass grafting. (2006)
  • Author(s): Takaba K, Jiang C, Saji Y, Nemoto S, Ikeda T, Urayama S, Azuma T, HokugoH, Tsutsumi S, Tabata Y, Komeda M.
  • Journal Title: J Thorac Cardiovasc Surg. Oct;132(4) Pages: 891-899
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A combination of omental flap and growth factor therapy induces arteriogenesis and increases myocardial perfusion in chronic myocardial ischemia : evolving concept of biologic coronary artery bypass grafting. (2006)
  • Author(s): Takaba K, Jiang C, Saji Y, Nemoto S, Ikeda T, Urayama S, Azuma T, Hokugo H, Tsutsumi S, Tabata Y, Komeda M.
  • Journal Title: The Journal of Thoracic Cardiovascular Surgery,2006 Oct 132(4) Pages: 891-99
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A combination of omental flap and growth factor therapy induces arteriogenesis and increases myocardial perfusion in chronic myocardial ischemia : evolving concept of biologic coronary artery bypass grafting. (2006)
  • Author(s): 鷹羽 浄顕
  • Journal Title: J Thorac Cardiovasc Surg. Oct;132(4) Pages: 891-899
• [Journal Article] Biologic Coronary Artery Bypass Grafting : A Combination of Omental Flap And Growth Factor Therapy Induces Arteriogenesis And Provides Excellent Myocardial Perfusion in Chronoic Ischemic Myocardium
  • Author(s): Takaba K, Jiang C, Saji Y, Nemoto S, Ikeda T, Urayama S, Azuma T, Hokugo H, Tsutsumi S, Tabata Y, Komeda M.
  • Journal Title: J Thorac Cardiovasc Surg (Submitted)