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Development of Tissue Engineered Heart Valve utilizing Autologous Bone Marrow Cells and Decellularized Homograft

Research Project

Project/Area Number 17591489
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionTokyo Women's Medical University

Principal Investigator

SAITO Satoshi  Tokyo Women's Medical University, School of Medicine, Associate Professor, 医学部, 講師 (60246551)

Co-Investigator(Kenkyū-buntansha) SHIN'OKA Toshiharu  Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (20192122)
KUROSAWA Hiromi  Tokyo Women's Medical University, School of Medicine, Professor, 医学部, 教授 (50075511)
ISHIYAMA Masakuni  Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (30246558)
YAMAMOTO Noboru  Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (50297470)
長津 正芳  東京女子医科大学, 医学部, 助教授 (60231487)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsRegenerative Medicine / Tissue Engineering / Tissue Engineered graft / Biodegradable Scaffold / Bone Marrow Cells / Decellularization / Homograft / Tissue Engineered valve / 再生心臓弁 / 細胞播種 / 内皮細胞
Research Abstract

Although in the hope of being the main resources of matrix for clinical application, pig aorta which is of high thickness have not been decellularized efficiently so far. Therefore the optimized decellularization protocol for pig aorta in this study based on Courtman's protocol was developed. DNA content test, Hematoxylin and eosin (HE) staining and immunohistological examination against a-actin, vimentin and desmin demonstrated the high decellularizaiton efficiency of the protocol. The high efficiency in cell removal didn't compromise the strength of the matrix which maintained mechanical strength comparable to that of the matrix from Courtman's protocol. Less immunoreaction and calcification in the matrix retrieved from subcutaneous model further proved the higher decellularization efficiency of the optimized protocol compare to the Courtman's protocol. In conclusion, we think the decellularization protocol present in this study is an ideal decellularization protocol for pig aorta.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2006 2005

All Journal Article (17 results) Book (1 results)

  • [Journal Article] Evaluation of Tissue-Engineered Vascular Autografts2006

    • Author(s)
      Matsumura Goki
    • Journal Title

      Tissue Engineering 12(11)

      Pages: 3075-3075

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 組織工学による心臓弁、血管再生2006

    • Author(s)
      宮本 真嘉
    • Journal Title

      医学のあゆみ 217(5)

      Pages: 424-424

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 先天性心疾患領域における再生医療・Tissue Engineeringの展望2006

    • Author(s)
      市原 有起
    • Journal Title

      小児外科 38(2)

      Pages: 239-239

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 培養人工血管-Tissue Engineering Graft-2006

    • Author(s)
      保々 恭子
    • Journal Title

      分子心血管病 7(5)

      Pages: 503-503

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Evaluation of Tissue-Engineered Vascular Autografts2006

    • Author(s)
      Goki Matsumura
    • Journal Title

      Tissue Engineering 12(11)

      Pages: 3075-3075

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 培養人工血管-TissueEngineering Graft-2006

    • Author(s)
      保々 恭子
    • Journal Title

      分子心血管病 7(5)

      Pages: 503-503

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005

    • Author(s)
      Shin' oka Toshiharu
    • Journal Title

      J Thorac Cardiovasc Surg 129(6)

      Pages: 1330-1338

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005

    • Author(s)
      Hibino Narutoshi
    • Journal Title

      J Thorac Cardiovasc Surg 129(5)

      Pages: 1064-1070

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005

    • Author(s)
      Toshiharu Shin' oka
    • Journal Title

      J Thorac Cardiovasc Surg 129(6)

      Pages: 1330-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005

    • Author(s)
      Narutoshi Hibino
    • Journal Title

      J Thorac Cardiovasc Surg 129(5)

      Pages: 1064-70

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005

    • Author(s)
      Shin'oka Toshiharu
    • Journal Title

      J Thorac Cardiovasc Surg 129(6)

      Pages: 1330-8

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005

    • Author(s)
      Hibino N, Shin'oka T, Matsumura G, Ikada Y, Kurosawa H
    • Journal Title

      J Thorac Cardiovasc Surg 129

      Pages: 1064-1070

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Autopericardial patch tracheoplasty for tracheal stenosis after arterial switch operation2005

    • Author(s)
      Takiguchi M, Aoki M, Shin'oka T, Hiramatsu T, Imai Y
    • Journal Title

      J Card Surg 20

      Pages: 244-246

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A successful total cavopulmonary connection conversion 13 years after anatomic repair for tricuspid atresia IIc with severe pulmonary resistance2005

    • Author(s)
      Sugimoto K, Kurosawa H, Shin'oka T, Nagatsu M, Morishima S
    • Journal Title

      J Thorac Cardiovasc Surg 129

      Pages: 684-685

    • Related Report
      2005 Annual Research Report
  • [Journal Article] One-stage intracardiac repair in combination with external stenting of the trachea and right bronchus for tetralogy of Fallot2005

    • Author(s)
      Sakamoto T, Nagase Y, Hasegawa H, Shin'oka T, Tomimatsu H, Kurosawa
    • Journal Title

      J Thorac Cardiovasc Surg 130

      Pages: 1717-1718

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Successful Application of Pulmonary Arterial Patch for Coronary Angioplasty Late after Arterial Switch Operation2005

    • Author(s)
      Kosaka Y, Shin'oka T, Yamazaki K, Kurosawa H
    • Journal Title

      Jpn J Thorac Cardiovasc Surg 53

      Pages: 601-603

    • NAID

      10016903731

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mid-term clinical results of tissue engineered vascular autografts seeded with autologous bome marrow cells2005

    • Author(s)
      Shin'oka T, Matsumura G, Hibino N, Naito Y, Watanabe M, Konuma T, Sakamoto T, Nagatsu M, Kurosawa
    • Journal Title

      J Thorac Cardiovasc Surg 129

      Pages: 1330-1338

    • Related Report
      2005 Annual Research Report
  • [Book] Cardiovascular Regeneration Therapies Using Tissue Engineering Approaches2005

    • Author(s)
      Matsumura G, Shin'oka T
    • Total Pages
      238
    • Publisher
      Springer
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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