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Cyte-physiological study of abnormal Ca^<2+> metabolism in ischemic and epileptic neuronal death

Research Project

Project/Area Number 17591527
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionJichi Medical University

Principal Investigator

OGURO Keiji  Jichi Medical University, Dep.Neurosurgery, Assistant professor, 医学部, 講師 (90231232)

Co-Investigator(Kenkyū-buntansha) YOKOTA Hidenori  Jichi Medical University, Dep.Neurosurgery, Assistant professor, 医学部, 講師 (90254929)
SHIMAZAKI Kuniko  Jichi Medical University, Dep.Neurosurgery, Assistant professor, 医学部, 講師 (40142153)
WATANABE Eiju  Jichi Medical University, Dep.Neurosurgery, Professor, 医学部, 教授 (50150272)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordscerebral ischemia / calcium / hippocampus / slice / EL mouse / epilepsy / IP_4
Research Abstract

Functional changes in IP_33kinase knock-out mice
IP_33-kinasemetabolizu IP_3 to 1,3,4,5-tetrakisphosphate (IP_4). Until recently physiological roles of IP_33-kinase or IP_4 are not well known. IP_33-kinase (A) is abundant in neuronal cells and IP_4 level in IP_33-kinase(A) deficient mice is significantly decreased. To know the role of IP_33-kinase or IP_4 in neuronal tissue, histochemical, behavioral and physiological study were performed using IP_33-kinase(A) deficient and wild type mice. In histochemical study with IP_33-kinase antibody, IP_33-kinase strongly express in hioppocampal CA1 region in normal gerbil. Passive avoidance with electric shocking chamber revealed less learning and memory function in IP_33-kinase(A) deficient mice. Hippocampal damage induced by global ischemia by 10 min common carotid occlusion did not show significant differences between IP_33-kinase(A) deficient and wild type mice. Intracellular Ca^<2+> increase following oxygen-glucose deprivation was also exam … More ined using hippocampal slice of deficient and wild type mice. These results suggest that IP_33-kinase and IP_4 have important roles in the process of learning and memory.
Enhanced excitability on hippocampal CA3 in EL mice
We observed the changes in intracellular calcium on EL and control DDY mice hippocampal slice after oxygen-glucose deprivation (OGD) using calcium imaging with Rhod2-AM. In EL mice, the cytosolic calcium on hippocampal CA1, CA3 was significantly increased after OGD. On the contrary control DDY mice showed increase just on CA1 region. We also performed the same experiments in developing animals (2-8w) to know from when the hyper excitability in CA3 can be seen. In developing EL mice, calcium increase after OGD was less than the adults evenly in CA1 and CA3. The fluorescent intensity was successively increased in both CA1 and CA3 according to its age. These results suggest that the enhanced excitability on hippocampal CA3 in EL mice is obtained gradually according to the age. It is not a postgrowth acquisition. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (22 results)

All 2007 2006 2005

All Journal Article (20 results) Book (2 results)

  • [Journal Article] てんかん外科の術前診断の現状2007

    • Author(s)
      横田英典, 小黒恵司, 渡辺英寿
    • Journal Title

      脳神経外科ジャーナル 16

      Pages: 164-170

    • Related Report
      2006 Annual Research Report
  • [Journal Article] わが国におけるてんかん外科の現状2007

    • Author(s)
      渡辺英寿
    • Journal Title

      脳神経外科速報 17

      Pages: 74-81

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Zinc-dependent multi-conductance channel activity in mitochondria isolated from ischemic brain.2006

    • Author(s)
      Bonanni L, Yokota H, Sensi SL, Zukin RS, Jonas EA, et al.
    • Journal Title

      J Neurosci 26

      Pages: 6851-6862

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Zinc-dependent multi-conductance channel activity in mitochondria isolated from ischemic brain2006

    • Author(s)
      Bonanni L, Yokota H, Sensi SL, Zukin RS, Jonas EA, et al.
    • Journal Title

      J Neurosci 26

      Pages: 6851-6862

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Elevation of ST2 protein leves in cerebrospinal fluid following subarachnoid hemorrhage2006

    • Author(s)
      Kanda M, Ohto-Ozaki H., Kuroiwa K, Tominaga S, Watanabe E, Iwashita H
    • Journal Title

      Acta Neurologica Scondinavica 113

      Pages: 327-333

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Zine-dependent multi-conductance channel activity in mitochondria Isolated from ischemic brain.2006

    • Author(s)
      Bonanni L, Yokota H, Sensi SL, Zukin RS, Jonas EA, et al.
    • Journal Title

      J Neurosci 26

      Pages: 6851-6862

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differential effects of sublethal ischemia and chemical preconditioning with 3-nitropropionic acid on protein expression in gerbil hippocampus.2005

    • Author(s)
      Kato K, Shimazaki K, Kamiya T, Amemiya S, Inaba T, Oguro K, Katayama Y
    • Journal Title

      Life Sciences 77

      Pages: 2867-2878

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Blockade of calcium-permeable AMPA receptors protects hippocampal neurons against global ischemia-induced death.2005

    • Author(s)
      Noh KM, Yokota H, Mashiko T, Castillo EP, Zukin RS, Bennett MVL
    • Journal Title

      Proc Natl Acad Sci U S A 102

      Pages: 12230-12235

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-apoptotic and neuroprotective effects of edaravone following transient global ischemia in rats2005

    • Author(s)
      Amemiya S, Kamiya T, Nito C, Inaba T, Kato K, Ueda M, Shimazaki K, Katayama Y
    • Journal Title

      Eur J Pharmacol 516

      Pages: 125-130

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Specific and efficient transduction of cochlear inner hair cells with recombinant adeno-associated virus type3 vector2005

    • Author(s)
      Liu Y, Okada T, Sheykholeslami K, Shimazaki K, Nomoto T, Muramatsu S, Kanazawa T, Takeuchi K, Ajalli R, Mizukami H, Kume A, Ichimura K, Ozawa K
    • Journal Title

      Mol Ther 12

      Pages: 725-733

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Large-scale production of recombinant viruses by use of a large culture vessel with active gassing.2005

    • Author(s)
      Okada T, Nomoto T, Yoshioka T, Nonaka-Sarukawa M, Ito T, Ogura T, Iwata-Okada M, Uchibori R, Shimazaki K, Mizukami H, Kume A, Ozawa K
    • Journal Title

      Hum Gene Ther 16

      Pages: 1212-1218

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Differential effects of sublethal ischemia and chemical preconditioning 3-nitropropionic acid on protein expression in gerbil hippocampus2005

    • Author(s)
      Kato K, Shimazaki K, Kamiya T, Amemiya S, Inaba T, Oguro K, Katayama Y.
    • Journal Title

      Life Sciences 77

      Pages: 2867-2878

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Blockade of calcium-permeable AMPA receptors protects hippocampal neurons against global ischemia-induced death2005

    • Author(s)
      Noh KM, Yokota H, Mashiko T, Castillo EP, Zukin RS, Bennett MVL.
    • Journal Title

      Proc Natl Acad Sci USA 102

      Pages: 12230-12235

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-apoptotic and neuroprotective effects of edaravone following transient global ischemia in rats2005

    • Author(s)
      Amemiya S, Kamiya T, Nito C, Inaba T, Kato K, Ueda M, Shimazaki K, Katayama Y.
    • Journal Title

      Eur J Pharmacol 516

      Pages: 125-130

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Specific and efficient transduction of cochlear inner hair cells with recombinant adeno-associated virus type3 vector2005

    • Author(s)
      Liu Y, Okada T, Sheykholeslami K, Shimazaki K, Nomoto T, Muramatsu S, Kanazawa T, Takeuchi K, Ajalli R, Mizukami H, Kume A, Ichimura K, Ozawa K.
    • Journal Title

      Mol Ther 12

      Pages: 725-733

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Large-scale production of recombinant viruses by use of a large culture vessel with active gassing2005

    • Author(s)
      Okada T, Nomoto T, Yoshioka T, Nonaka-Sarukawa M, Ito T, Ogura T, Iwata-Okada M, Uchibori R, Shimazaki K, Mizukami H, Kume A, Ozawa K.
    • Journal Title

      Hum Gene Ther 16

      Pages: 1212-1218

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Differential effects of sublethal ischemia and chemical preconditioning with 3-nitropropionic acid on protein expression in gerbil hippocampus.2005

    • Author(s)
      Kato K, Shimazaki K, Kamiya T, Amemiya S, Inaba T, Oguro K, Katayama Y
    • Journal Title

      Life Science 77

      Pages: 2867-2878

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats.2005

    • Author(s)
      Amemiya S, Kamiya T, Nito C, Inaba T, Kato K, Ueda M, Shimazaki K, Katayama Y
    • Journal Title

      Eur J Pharmacol 516

      Pages: 125-130

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Specific and Efficient Transduction of Cochlear Inner Hair Cells with Recombinant Adeno-Associated Virus Type 3 Vector.2005

    • Author(s)
      Liu Y, Okada, Sheykholeslami K, Shimazaki K, Nomoto T, Muramatsu S, Kanazawa T, Takeuchi K, Ajalli R, Mizukami H, Kume A, Ichimura K, Ozawa K
    • Journal Title

      Mol Ther. 12

      Pages: 725-733

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Large-scale production of recombinant viruses by use of a large culture vessel with active gassing.2005

    • Author(s)
      Okada T, Nomoto T,, Yoshioka T, Nonaka-Sarukawa M, Ito T, Ogura T, Iwata-Okada, M, Uchibori R, Shimazaki K, Kume A, Ozawa K
    • Journal Title

      Hum Gene Ther 16

      Pages: 1212-1218

    • Related Report
      2005 Annual Research Report
  • [Book] イオンチャンネルを共有する受容体.グルタミン酸 in生物薬科学実験講座-情報伝達物質[I]受容体-(石橋貞彦,市川 厚,堅田利明編集)(廣川書店)2005

    • Author(s)
      島崎久仁子, 川合述史
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] イオンチャネルを共有する受容体.グルタミン酸.In:生物薬科学実験講座- 情報伝達物質 [I]受容体-(石橋貞彦, 市川厚, 堅田利明 編)2005

    • Author(s)
      島崎久仁子, 川合述史
    • Publisher
      廣川書店、東京
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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