| Project/Area Number |
17591543
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KONDO Eiji Hokkaido Univ., Creative Research Initiative Sousei., Asso. Prof, 創成科学協同研究機構, 特任助教授 (60374724)
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| Co-Investigator(Kenkyū-buntansha) |
YASUDA Kazunori Hokkaido Univ., Grad.School of Med., Prof, 大学院医学研究科, 教授 (20166507)
TOHYAMA Harukazu Hokkaido Univ., Grad.School of Med., Asso.Prof, 大学院医学研究科, 助教授 (60301884)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Tendon / Matrix / Remodeling / Graft / Ligament / Synovial cell / Cell therapy / Anterior cruciate ligament / 腱移植 |
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| Research Abstract |
Purpose
The present study was conducted to clarify the effect of cell therapy with autologous synovial tissue-derived fibroblasts activated by transforming growth factor (TGF)-betal on the necrotized anterior cruciate ligament (ACL).
Materials and Methods
Thirty-six mature female Japanese White rabbits were used in this study. In Group I, the fibrin glue with autologous synovial tissue-derived fibroblasts after TGF-beta stimulation was wrapped around the necrotized ACL after the freeze-thaw treatment. In Group II, the one without TGF-beta stimulation was wrapped around the frozen-thawed ACL. In Group III, the fibrin glue without fibroblasts was applied in the same manner on the frozen-thawed ACL.
Results
Histological observation found that implantation of fibroblasts after TGF-beta stimulation accelerated cellular infiltration into the ACL following fibroblast necrosis. Biomechanically, the transplantation of synovial tissue-derived autologous fibroblasts activated by TGF-beta inhibited mechanical deterioration of the ACL after the freeze-thaw treatment.
Discussion
The present study has shown that the cell-based therapy using synovial tissue-derived fibroblasts activated by TGF-beta1 is one of possible solutions against mechanical deterioration of the graft after ACL reconstruction.
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