| Project/Area Number |
17591574
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
TANAKA Nobuhiho Hiroshima University, Hospital, Assistant, 病院, 助手 (20363062)
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| Co-Investigator(Kenkyū-buntansha) |
OCHI Mitsuo Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院医歯薬学総合研究科, 教授 (70177244)
YASUNAGA Yuji Hiroshima University, Graduate School of Biomedical Sciences, Visiting professor, 大学院医歯薬学総合研究科, 客員教授 (40253075)
MOCHIZUKI Yu Hiroshima University, Hospital, Associate professor, 病院・助教授 (10284192)
石田 治 広島大学, 大学院・医歯薬学総合研究科, 助教授 (00243551)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | neural progenitor cell / magnetic beads / cell delivery system / axon growth / co-culture / spinal cord injury |
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| Research Abstract |
In order to develop the neural progenitor cell magnetic targeting, a new method to form the magnetically labeled NPCs using RGDS peptide is established. Labeled NPCs preserved the characteristics of non-labeled NPCs and they also have the potential to be localised by magnetic force. (Neuro Report 2005, Characterization of labeled neural progenitor cells for magnetic targeting)
And to assess the corticospinal axon growth potential in varying concentrations of NPCs and in magnetically localized labeled NPCs, quantitatively using our original organotypic co-culture system. Differential potentials were not changed whether labeled NPCs were localized or scattered in vitro. Corticospinal axon growth was promoted in accordance with the transplanted NPC numbers around the organotypic co-culture. Labeled NPCs had almost the same potential for axon growth as non-labeled NPCs. Labeled NPCs localized by magnetic force promoted axon growth much more than scattered Labeled NPCs without a magnet. (Spine in press, Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote axon growth in organotypic co-cultures)
Of course, further studies of spinal cord injury models are necessary, magnetic targeting systems using these labeled NPCs have the potential to be a successful administration method of NPCs. The magnetic targeting of labeled NPCs suggests the potential utility as a prospective therapeutic approach in spinal cord injury.
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