Pathology and treatment for osteoarthritis-efficient use of endogenous insulin-like growth factor-1
| Project/Area Number |
17591582
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
MATSUMOTO Tomoko Nagasaki University, Graduate School of Biomedical Sciences, Associate professor, 大学院医歯薬学総合研究科, 助教授 (30239107)
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| Co-Investigator(Kenkyū-buntansha) |
OSAKI Makoto Nagasaki University, Graduate School of Biomedical Sciences, Assistant professor, 大学院医歯薬学総合研究科, 助手 (20380959)
SHINDO Hiroyuki Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院医歯薬学総合研究科, 教授 (30107677)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Osteoarthritis / Insulin-like growth factor / articular cartilage / cartilage repair / IGF-結合蛋白 |
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| Research Abstract |
The aim of this study was to clarify the effects of insulin-like growth factor (IGF)-I on cartilage metabolism, and finally to investigate the treatment for osteoarthritis. IGF-I was added to the culture medium of human articular chondrocytes with/without interleukin (IL)-1 or dexamethasone. Total RNA was extracted from the cell layer, and mRNA expression for aggrecan, type II collagen, matrix metalloprotease (MMP), IGF-I, IGF-binding proteins, and IGF-I receptor was examined using RT-PCR and real time-PCR. IGF-I induced the expression of aggrecan and type II collagen mRNA, and slightly decreased the inhibitory effect of IL-I or dexamethasone on mRNA expression for aggrecan. IGF-I showed little effects on MMP-3, -9, 13 expression. Further IL-1 induced the expression of IGF-I but decreased IGF-receptor. On the contrary, dexamethasone induced IGF-1 receptor but decreased IGF-I expression. mRNA expression for IGF-binding proteins was also regulated by both f IL-1 and dexamethasone These findings suggest that IGF-I promote synthesis of cartilage matrix, and protect articular cartilage from degradation. IGF-I might be one of the candidates for treatment of osteoarthritis.
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Report
(3 results)
Research Products
(13 results)
