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Pathology and treatment for osteoarthritis-efficient use of endogenous insulin-like growth factor-1

Research Project

Project/Area Number 17591582
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionNagasaki University

Principal Investigator

MATSUMOTO Tomoko  Nagasaki University, Graduate School of Biomedical Sciences, Associate professor, 大学院医歯薬学総合研究科, 助教授 (30239107)

Co-Investigator(Kenkyū-buntansha) OSAKI Makoto  Nagasaki University, Graduate School of Biomedical Sciences, Assistant professor, 大学院医歯薬学総合研究科, 助手 (20380959)
SHINDO Hiroyuki  Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院医歯薬学総合研究科, 教授 (30107677)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsOsteoarthritis / Insulin-like growth factor / articular cartilage / cartilage repair / IGF-結合蛋白
Research Abstract

The aim of this study was to clarify the effects of insulin-like growth factor (IGF)-I on cartilage metabolism, and finally to investigate the treatment for osteoarthritis. IGF-I was added to the culture medium of human articular chondrocytes with/without interleukin (IL)-1 or dexamethasone. Total RNA was extracted from the cell layer, and mRNA expression for aggrecan, type II collagen, matrix metalloprotease (MMP), IGF-I, IGF-binding proteins, and IGF-I receptor was examined using RT-PCR and real time-PCR. IGF-I induced the expression of aggrecan and type II collagen mRNA, and slightly decreased the inhibitory effect of IL-I or dexamethasone on mRNA expression for aggrecan. IGF-I showed little effects on MMP-3, -9, 13 expression. Further IL-1 induced the expression of IGF-I but decreased IGF-receptor. On the contrary, dexamethasone induced IGF-1 receptor but decreased IGF-I expression. mRNA expression for IGF-binding proteins was also regulated by both f IL-1 and dexamethasone These findings suggest that IGF-I promote synthesis of cartilage matrix, and protect articular cartilage from degradation. IGF-I might be one of the candidates for treatment of osteoarthritis.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report

Research Products

(13 results)

All 2006 2005

All Journal Article (13 results)

  • [Journal Article] 【関節炎マーカーの基礎と臨床】COMP(cartilage oligomeric matrix protein)2006

    • Author(s)
      松本智子
    • Journal Title

      リウマチ科 35(6)

      Pages: 559-564

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interleukin-6 levels in synovial fluids of patients with rheumatoid arthritis correlated with the infiltration of inflammatory cells in synovial membrane2006

    • Author(s)
      Matsumoto T, Tsurumoto T, Shindo H
    • Journal Title

      Rheumatol Int 26

      Pages: 1096-1100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Effeets of insulin-like growth factor I on transforming growth factor betal induced chondrogenesis of synovium-derived mesenchymal stem cells cultured in a polyglycolic acid scaffold2006

    • Author(s)
      Sakimura K, Matsumoto T, et al.
    • Journal Title

      Cells Tissues Organs 183

      Pages: 55-61

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cartilage Oligomeric Matrix protein2006

    • Author(s)
      Matsumoto T
    • Journal Title

      Rheumatology 35

      Pages: 559-564

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of insulin-like growth factor I on transforming growth factor beta1 induced chondrogenesis of synovium-derived mesenchymal stem cells cultured in a polyglycolic acid scaffold2006

    • Author(s)
      Sakimura K, Matsumoto T, et al.
    • Journal Title

      Cells Tissues Organs 183

      Pages: 55-61

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 【関節炎マーカーの基礎と臨床】COMP (cartilage oligomeric matrix protein)2006

    • Author(s)
      松本智子
    • Journal Title

      リウマチ科 35(6)

      Pages: 559-564

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of insulin-like growth factor I on transforming growth factor betal induced chondrogenesis of synovium-derived mesenchymal stem cells cultured in a polyglycolic acid scaffold2006

    • Author(s)
      Sakimura K, Matsumoto T, et al.
    • Journal Title

      Cell Tissues Organs 183

      Pages: 55-61

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enhanced expression of insulin-like growth factor-binding proteins in human osteoarthritic cartilage detected by immunohistochemistry and in situ hybridization.2005

    • Author(s)
      Iwanaga H, Matsumoto T, et al.
    • Journal Title

      Osteoarthritis Cartilage 13

      Pages: 439-448

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of polyethylene particles isolated from periprosthetic tissue of loosened hip arthroplasty and comaprison with radiographic appearance.2005

    • Author(s)
      Koseki H, Matsumoto T. et al.
    • Journal Title

      J Orthopaedic Science 10

      Pages: 284-290

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis2005

    • Author(s)
      Yabe Y, Matsumoto T. et al.
    • Journal Title

      J Orthop Sci 10

      Pages: 589-594

    • NAID

      10017080350

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of polyethylene particles isolated from periprosthetic tissue of loosened hip arthroplasty and comaprison with radiographic appearance.2005

    • Author(s)
      Koseki H, et al.
    • Journal Title

      J Orthopaedic Science 10

      Pages: 284-290

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Enhanced expression of insulin-like growth factor-binding proteins in human osteoarthritic cartilage detected by immunohistochemistry and in situ hybridization.2005

    • Author(s)
      Iwanaga H, et al.
    • Journal Title

      Osteoarthritis Cartilage 13

      Pages: 439-448

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis.2005

    • Author(s)
      Yabe Y et al.
    • Journal Title

      J Orthop Sci. 10

      Pages: 589-594

    • Related Report
      2005 Annual Research Report

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Published: 2005-03-31   Modified: 2016-04-21  

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