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Basic research for anti-biofilms drugs discovery

Research Project

Project/Area Number 17591583
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionNagasaki University

Principal Investigator

TSURUMOTO Toshiyuki  Nagasaki University, Hospital of Medicine and Dentistry, Assistant Professor (60304937)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Tomoko  Nagasaki University, Graduate school of Biomedical Science, Associate Professor (30239107)
HIRAKATA Yoichi  Nagasaki University, Hospital, Assistant Professor (50238341)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsinfection / bacteria / pharmacology / bionfilm / osteomyelitis / antibiotics / sensitibitv / biomaterial
Research Abstract

Implant-related infection after orthopedic surgery is difficult to cure. One of the causes of infection is the bacterial biofilm that forms around biomaterials used during surgery. Therefore, it is necessary to investigate bacterial biofilms extensively to resolve the problems of these postoperative infections. However, no established culture method or quantification system exists for bacterial biofilms grown on the surface of the metallic biomaterials used in orthopedics, which are non radio lucent. The purpose of this study was to develop a quantitative method to evaluate the difference in resistance of stainless steel versus titanium to staphylococcal biofilms and the efficacy of antibiotics against biofilms.
The bacterial strains used in this study were three Staphylococcus aureus stains: strain Seattle 1945 and two clinical strains cultured from postoperative infections. Staphylococcal biofilms were formed on stainless steel washers (SUS304) and titanium washers (pure titanium). They were stained with crystal violet and were examined with a digital microscope to calculate the bacterial coverage rate (BCR) by NIH imaging.
The BCR of S. aureus biofi lms formed on stainless steel and titanium washers increased over time. The amount of biofilm on the surface of the stainless steel washers was significantly greater or tended to be greater than that on the titanium.
The newly developed quantitative method (static microtube culture and measurement system) was useful for assessing the amount of bacterial biofilms on the surface of nontranslucent biomaterial. We found that titanium may be more resistant to bacterial infection than stainless steel. To control implant-related severe infections, the biomaterials should be assessed from the viewpoint of their resistibility to bacterial adhesions and infections.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (36 results)

All 2007 2006 2005

All Journal Article (17 results) (of which Peer Reviewed: 2 results) Presentation (18 results) Book (1 results)

  • [Journal Article] New quantitative image analysis of staphylococcal biofilms on the surfaces of nontranslucent metallic biomaterials2007

    • Author(s)
      Adachi K, Tsurumoto T, et. al.
    • Journal Title

      Jorunal of orthopaedic science 12

      Pages: 157-160

    • NAID

      10019572462

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 金属表面の黄色ブドウ球菌バイオフィルム形成と培養条件2007

    • Author(s)
      梶山 史郎, 弦本 敏行, 他
    • Journal Title

      日本骨関節感染症学会雑誌 20

      Pages: 93-96

    • NAID

      40015482938

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 骨関節感染症治療の進展感染症成立の背景一起炎菌とバイオフィルム2007

    • Author(s)
      弦本 敏行
    • Journal Title

      関節外科 26

      Pages: 163-165

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] New quantitative image analysis of staphylococcal biofilms on the surfaces of nontranslucent metallic biomaterials.2007

    • Author(s)
      K. Adachi, T. Tsurumoto, et. al.
    • Journal Title

      Journal of Orthopaedic Science 12

      Pages: 178-84

    • NAID

      10019572462

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Staphylococcus aureus biofilms on metallic surface(Japanese)2007

    • Author(s)
      S. Kajiyama, T. Tsurumoto., et. al.
    • Journal Title

      Nihon kotsu kansetsu kansensho gakkai zasshi 20

      Pages: 93-96

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Background of bacterial infection at bone and joint(Japanese)2007

    • Author(s)
      T. Tsurumoto
    • Journal Title

      Kansetsu geka 26

      Pages: 163-165

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] New quantitative image analysis of staphylococcal biofi lma on the surfaces of nontranslucent metallic biomaterials2007

    • Author(s)
      Adachi K, Tsurumoto T, et al.
    • Journal Title

      Journal of Orthopaedic Science 12(in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 金属表面の黄色ブドウ球菌バイオフィルム形成と培養条件2007

    • Author(s)
      梶山史郎, 弦本 敏行, ほか
    • Journal Title

      日本骨関節感染症学会雑誌 20

      Pages: 93-96

    • NAID

      40015482938

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Antimicrobial susceptibility of Staphylococcus aureus and Staphylococcus epidermidis biofilms isolated from infected total hip arthroplasty cases2006

    • Author(s)
      Nishimura S., Tsurumoto T, et. al.
    • Journal Title

      Jorunal of orthopaedic science 11

      Pages: 45-50

    • NAID

      10017328805

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 細菌への時間の暴露差異におけるBiofilm形成の定量2006

    • Author(s)
      安達 耕一, 弦本 敏行, 他
    • Journal Title

      日本骨・関節感染症研究会雑誌 19

      Pages: 52-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antimicrobial susceptibility of Staphylococcus aureus and Staphylococcus epidermidis biofilms isolated from infected total hip arthroplasty cases2006

    • Author(s)
      S. Nishimura, T. Tsurumoto, et. al.
    • Journal Title

      Journal of Orthopaedic Science 11

      Pages: 46-50

    • NAID

      10017328805

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantification of bacterial biofilms(Japanese)2006

    • Author(s)
      K. Adachi, T. Tsururnoto, et. al.
    • Journal Title

      Nihon kotsu kansetsu kansensho kennkyukai zasshi 19

      Pages: 52-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antimicrobial susceptibility of Staphlococcus aureus and Staphylococcus epidermidis biofilms isolated from infected total hip arthroplasty cases2006

    • Author(s)
      Nishimura S, Tsurumoto T, et al.
    • Journal Title

      Journal of Orthopaedic Science 11

      Pages: 46-50

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 【骨・関節感染症の診断と治療】骨・関節感染症の臨床検査法2005

    • Author(s)
      弦本 敏行
    • Journal Title

      リウマチ科 33

      Pages: 580-585

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Laboratory data on bone and joint infection(Japanese)2005

    • Author(s)
      T. Tsurumoto
    • Journal Title

      Riumachi-ka 33

      Pages: 580-585

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Guideline for microbiological laboratory test for bacterial infection(Japanese)2005

    • Author(s)
      T. Tsurumoto
    • Journal Title

      Bunko-do

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 細菌への暴露時間の差異におけるBiofilm形成の定量2005

    • Author(s)
      安達耕一, 弦本敏行, ほか
    • Journal Title

      日本骨・関節感染症学会雑誌 19

      Pages: 52-55

    • NAID

      40015467822

    • Related Report
      2005 Annual Research Report
  • [Presentation] Antibacterial Orthopaedic Metal Surface with Photocatalytic Bactericidal Effect of TiO2 on Staphylococcus aureus2007

    • Author(s)
      K.Shiraishi, H.Kloseki, T.Tsurumoto, K.Baba, et. al.
    • Organizer
      1st Asian Biomaterials Congress
    • Place of Presentation
      Tsukuba,Japan
    • Year and Date
      2007-12-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Antibacterial Orthopaedic Metal Surface with Photocatalytic Bactericidal Effect of TiO2 on Staphylococcus aureus2007

    • Author(s)
      K. Shiraishi, H. Koseki, T. Tsurumoto, et. al.
    • Organizer
      1st Asian Biomaterials Congress
    • Place of Presentation
      Tsukuba
    • Year and Date
      2007-12-06
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] 表皮ブドウ球菌バイオフィルム形成過程における金属表面占拠率と生物学的活性の経時的評価2007

    • Author(s)
      梶山 史郎, 弦本 敏行, ほか
    • Organizer
      第22回目本整形外科学会基礎学術集会
    • Place of Presentation
      浜松市
    • Year and Date
      2007-10-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] 酸化チタン光触媒作用による金属表面への抗菌性付与2007

    • Author(s)
      白石 公太郎, 小関 弘展, 弦本 敏行, ほか
    • Organizer
      第22回日本整形外科学会基礎学術集会
    • Place of Presentation
      浜松市
    • Year and Date
      2007-10-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Bacterial coverage rate and biological activity of S. epidermidis on biofilms2007

    • Author(s)
      S. Kajiyama, T. Tsurumoto, et. al.
    • Organizer
      22th Annual research meeting of the Japanese Orthopaedic Association
    • Place of Presentation
      Hamamatsu
    • Year and Date
      2007-10-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Antibacterial Orthopaedic Metal Surface with Photocatalytic Bactericidal Effect of TiO2 on Staphylococcus aureus2007

    • Author(s)
      K. Shiraishi, H. Koseki, T. Tsurumoto, et. al.
    • Organizer
      22th Annual research meeting of the Japanese Orthopaedic Association
    • Place of Presentation
      Hamamatsu
    • Year and Date
      2007-10-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] 金属表面における表皮ブドウ球菌バイオフィルム形成初期過程の評価2007

    • Author(s)
      梶山 史郎, 弦本 敏行, ほか
    • Organizer
      第30回日本骨・関節感染症学会
    • Place of Presentation
      山梨県北杜市
    • Year and Date
      2007-06-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] 酸化チタン光触媒作用による金属表面への抗菌性付与2007

    • Author(s)
      白石 公太郎, 小関 弘展, 弦本 敏行, ほか
    • Organizer
      第30回日本骨・関節感染症学会
    • Place of Presentation
      山梨県北杜市
    • Year and Date
      2007-06-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] quantification of bacterial biofilms in early stage2007

    • Author(s)
      S. Kajiyama, T. Tsurumoto, et. al.
    • Organizer
      30th Annual research meeting of the Japanese Society for Study of Bone and Joint Infections
    • Place of Presentation
      Kitamori
    • Year and Date
      2007-06-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Antibacterial Orthopaedic Metal Surface with Photocatalytic Bactericidal Effect of TiO2 on Staphylococcus aureus2007

    • Author(s)
      K. Shiraishi, H. Koseki, T. Tsurumoto, et. al.
    • Organizer
      30th Annual research meeting of the Japanese Society for Study of Bone and Joint Infections
    • Place of Presentation
      Kitamori
    • Year and Date
      2007-06-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] メタル(非透過粧体材料)表面におけ宕バイオフィルムの趨的解析2006

    • Author(s)
      安達 耕一, 弦本 敏行, ほか
    • Organizer
      第21回日本整形外科学会基礎学術集
    • Place of Presentation
      長崎市
    • Year and Date
      2006-10-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Microtiter MethodとWasherを用いたStatic Culture Methodの比較2006

    • Author(s)
      梶山 史郎, 弦本 敏行, ほか
    • Organizer
      第21回日本整形外科学会基礎学術集
    • Place of Presentation
      長崎市
    • Year and Date
      2006-10-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Quantification of bacterial biofilms on metallic biomaterials2006

    • Author(s)
      K. Adachi, T. Tsurumoto, et. al.
    • Organizer
      21th Annual research meeting of the Japanese Orthopaedic Association
    • Place of Presentation
      Nagasaki
    • Year and Date
      2006-10-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Comparison of microtiter method and static culture method2006

    • Author(s)
      S. Kajiyama, T. Tsurumoto, et. al.
    • Organizer
      21th Annual research meeting of the Japanese Orthopaedic Association
    • Place of Presentation
      Nagasaki
    • Year and Date
      2006-10-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] 細菌への暴露時間の差異におけるBiofilm形成の定量2006

    • Author(s)
      安達 耕一, 弦本 敏行, ほか
    • Organizer
      第29回日本骨・関節感染症研究会
    • Place of Presentation
      米子市
    • Year and Date
      2006-06-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Quantification of bacterial biofilms2006

    • Author(s)
      K. Adachi, T. Tsurumoto, et. al.
    • Organizer
      29th Annual research meeting of the Japanese Society for Study of Bone and Joint Infections
    • Place of Presentation
      Yonago
    • Year and Date
      2006-06-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Static Micro-tube MethodによるBiofilmの形成および定量的評価2005

    • Author(s)
      安達 耕一, 弦本 敏行, ほか
    • Organizer
      第20回日本整形外科学会基礎学術集会
    • Place of Presentation
      伊勢市
    • Year and Date
      2005-10-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Quantification of bacterial biofilms by static-micro tube method2005

    • Author(s)
      K.Adachi, T. Tsurumoto, et. al.
    • Organizer
      20th Annual research meeting of the Japanese Orthopaedic Association
    • Place of Presentation
      Ise
    • Year and Date
      2005-10-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] ベッドサイドで役立つ微生物検査ガイド9.整形外科領域の感染症が疑われたとき2006

    • Author(s)
      弦本 敏行
    • Total Pages
      5
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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