| Project/Area Number |
17591583
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
TSURUMOTO Toshiyuki Nagasaki University, Hospital of Medicine and Dentistry, Assistant Professor (60304937)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Tomoko Nagasaki University, Graduate school of Biomedical Science, Associate Professor (30239107)
HIRAKATA Yoichi Nagasaki University, Hospital, Assistant Professor (50238341)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | infection / bacteria / pharmacology / bionfilm / osteomyelitis / antibiotics / sensitibitv / biomaterial |
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| Research Abstract |
Implant-related infection after orthopedic surgery is difficult to cure. One of the causes of infection is the bacterial biofilm that forms around biomaterials used during surgery. Therefore, it is necessary to investigate bacterial biofilms extensively to resolve the problems of these postoperative infections. However, no established culture method or quantification system exists for bacterial biofilms grown on the surface of the metallic biomaterials used in orthopedics, which are non radio lucent. The purpose of this study was to develop a quantitative method to evaluate the difference in resistance of stainless steel versus titanium to staphylococcal biofilms and the efficacy of antibiotics against biofilms.
The bacterial strains used in this study were three Staphylococcus aureus stains: strain Seattle 1945 and two clinical strains cultured from postoperative infections. Staphylococcal biofilms were formed on stainless steel washers (SUS304) and titanium washers (pure titanium). They were stained with crystal violet and were examined with a digital microscope to calculate the bacterial coverage rate (BCR) by NIH imaging.
The BCR of S. aureus biofi lms formed on stainless steel and titanium washers increased over time. The amount of biofilm on the surface of the stainless steel washers was significantly greater or tended to be greater than that on the titanium.
The newly developed quantitative method (static microtube culture and measurement system) was useful for assessing the amount of bacterial biofilms on the surface of nontranslucent biomaterial. We found that titanium may be more resistant to bacterial infection than stainless steel. To control implant-related severe infections, the biomaterials should be assessed from the viewpoint of their resistibility to bacterial adhesions and infections.
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