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Functional analysis of newly collagen (mouse Co124a1) expressed in bone tissues

Research Project

Project/Area Number 17591584
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionOita University

Principal Investigator

MATSUO Noritaka  Oita University, Department of Medicine, Assistant Professor, 医学部, 助手 (10284788)

Co-Investigator(Kenkyū-buntansha) SUMIYOSHI Hideaki  Oita University, Medicine, Assistant Professor, 医学部, 助手 (60343357)
HAMANAKA Ryoji  Oita University, Medicine, Assistant Professor, 医学部, 助手 (60274750)
YOSHIOKA Hidekatsu  Oita University, Medicine, Professor, 医学部, 教授 (00222430)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsCollagen / Bone tissue / Transcription / Bone differentiation
Research Abstract

Mouse col24al encodes a polypeptide chain of 1733 amino acids, and the overall structure is conserved. Oligo-CAP Race indicated that mouse col24al gene has at least two major splicing variants. Cell transfection experiments in combination with DNA-binding assays demonstrated that Col24al promoter activity is under the control of an upstream cis-acting element, which is shared by both transcripts and is recognized by specific combinations of c-Jun, CREB1, ATF1 and ATF2 dimers. Consistent with these results, overexpression of c-Jun, ATF1, ATF2 or CREB1 in transiently transfected osteoblastic cells stimulated transcription from reporter gene constructs driven by the Col24al promoter to different degrees. Moreover, chromatin immunoprecipitation experiments showed that these nuclear factors bind the same upstream sequence of the endogenous Co124a1 gene.
In situ hybridization on Neonatal (p4) tibia confirmed that col24al is expressed in bone shaft, but it is not expressed in cartilage or growth plates. In accordance with these in vivo observations, several osteoblast lineage cell lines, such as ROS17 and MC3T3-E1, and primary calvarial osteoblast, were found to express col24al. Furthermore, col24al expression was activated during BMP-2 induced differentiation of C3H10T1/2 mesenchymal cells.
Collectively these data provide new information about transcriptional control of collagen fibrillogenesis, in addition to implicating for the first time CREB/AP1 protein complexes in the regulation of collagen gene expression in osteoblasts.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (24 results)

All 2006 2005 Other

All Journal Article (24 results)

  • [Journal Article] CREB/AP1 protein complexs regulate transcription of the collagen XXIV gene (Co124al) in osteoblasts.2006

    • Author(s)
      N.Matsuo, S.Tanaka et al.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 5445-5452

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multifactor complex containing B element binding factor, BBF, and repressors regulate the human a1(III) collagen gene (COL3A1).2006

    • Author(s)
      H.Sumiyoshi, N.Matsuo et al.
    • Journal Title

      Acta Medica Okayama. 60

      Pages: 181-189

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] CREB/AP1 protein complexs regulate transcription of the collagen XXIV gene (Col24al) in osteoblasts.2006

    • Author(s)
      N.Matsuo, S.Tanaka, M.K.Gordon, M.Koch, H.Yoshioka, F.Ramirez
    • Journal Title

      J. Biol. Chem. 281

      Pages: 5445-5452

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multifactor complex containing B element binding factor, BBF, and repressors regulate the human al(III) collagen gene (COL3A1).2006

    • Author(s)
      H.Sumiyoshi, N.Matsuo, T.Shin, K.Shirabe, H.Yoshioka
    • Journal Title

      Acta Medica Okayama. 60

      Pages: 181-189

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] CREB/AP1 protein complexs regulate transcription of the collagen XXIV gene (Col24al) in osteoblasts2006

    • Author(s)
      Noritaka Matsuo et al.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 5445-52

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Multifactor complex containing B element binding factor, BBF, and repressors regulate the human al (III) collagen gene (COL3A1)2006

    • Author(s)
      Hideaki Sumiyoshi et al.
    • Journal Title

      Acta Medica Okayama 60

      Pages: 181-9

    • Related Report
      2006 Annual Research Report
  • [Journal Article] CREB/AP1 protein complexs regulate transcription of the collagen XXIV gene (Col24a1) in osteoblasts2006

    • Author(s)
      Noritaka Matsuo et al.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 5445-5452

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Adiponectin is expressed in the brown adipose tissue and surrounding immature tissues in mouse embryos.2005

    • Author(s)
      N.Fujimoto, N.Matsuo, et al.
    • Journal Title

      Biochim Biophys Acta 1731

      Pages: 1-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] コラーゲンと臓器線維症2005

    • Author(s)
      吉岡秀克, 松尾哲孝
    • Journal Title

      西日本泌尿器科 67

      Pages: 176-184

    • NAID

      10016901566

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] MN-cadherin and its novel variant are transiently expressed in chick embryo spinal cord2005

    • Author(s)
      K.Shirabe, Y.Kimura, et al.
    • Journal Title

      Biochem Biophys Res Commun. 334

      Pages: 108-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pro-alpha3(V) collagen chain is expressed in bone and its basic N-terminal peptide adheres to osteosarcoma cells.2005

    • Author(s)
      K.Yamaguchi, N.Matsuo et al.
    • Journal Title

      Matrix Biol. 24

      Pages: 283-294

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multiple proteins are involved in the protein-DNA complex in the proximal promoter of the human alphal(III) collagen gene (COL3A1).2005

    • Author(s)
      T.Yoshino, H.Sumiyoshi et al.
    • Journal Title

      Biochim Biophys Acta. 1729

      Pages: 94-104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sp1 and Sp3 transcription factors upregulate the proximal promoter of the human prostate-specific antigen gene in prostate cancer cells.2005

    • Author(s)
      T.Shin, H.Sumiyoshi et al.
    • Journal Title

      Arch Biochem Biophys 435

      Pages: 94-104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adiponectin is expressed in the brown adipose tissue and surrounding immature tissues in mouse embryos.2005

    • Author(s)
      N.Fujimoto, N.Matsuo, H.Sumiyoshi, K.Yamaguchi, T.Saikawa, H.Yoshimatsu, H.Yoshioka
    • Journal Title

      Biochim Biophys Acta. 1731

      Pages: 1-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] MN-cadherin and its novel variant are transiently expressed in chick embryo spinal cord.2005

    • Author(s)
      K.Shirabe, Y.Kimura, N.Matsuo, M.Fukushima, H.Yoshioka, H.Tanaka
    • Journal Title

      Biochem Biophys Res Commun. 334

      Pages: 108-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pro-alpha3(V) collagen chain is expressed in bone and its basic N-terminal peptide adheres to osteosarcoma cells.2005

    • Author(s)
      K.Yamaguchi, N.Matsuo, H.Sumiyoshi, N.Fujimoto, K.Iyama, H.Yoshioka
    • Journal Title

      Matrix Biol. 24

      Pages: 283-294

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Multiple proteins are involved in the protein-DNA complex in the proximal promoter of the human alpha 1 (III) collagen gene (COL3A1).2005

    • Author(s)
      T.Yoshino, H.Sumiyoshi, T.Shin, N.Matsuo, Y.Inagaki, Y.Ninomiya, H.Yoshioka
    • Journal Title

      Biochim Biophys Acta. 1729

      Pages: 94-104

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sp1 and Sp3 transcription factors upregulate the proximal promoter of the human prostate-specific antigen gene in prostate cancer cells.2005

    • Author(s)
      T.Shin, H.Sumiyoshi, N.Matsuo, F.Satoh, Y.Nomura, H.Mimata, H.Yoshioka
    • Journal Title

      Arch Biochem Biophys. 435

      Pages: 291-302

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adiponectin is expressed in the brown adipose tissue and surrounding immature tissues in mouse embryos.2005

    • Author(s)
      Noritaka Fujimoto et al.
    • Journal Title

      Biochim Biophys Acta. 1731

      Pages: 1-12

    • Related Report
      2005 Annual Research Report
  • [Journal Article] コラーゲンと臓器線維症2005

    • Author(s)
      吉岡秀克 et al.
    • Journal Title

      西日本泌尿器科雑誌 67

      Pages: 176-184

    • NAID

      10017397452

    • Related Report
      2005 Annual Research Report
  • [Journal Article] MN-cadherin and its novel variant are transiently expressed in chick embryo spinal cord.2005

    • Author(s)
      Komei Shirabe et al.
    • Journal Title

      Biochem Biophys Res Commun, 334

      Pages: 108-116

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Pro-alpha3(V) collagen chain is expressed in bone and its basic N-terminal peptide adheres to osteosarcoma cells.2005

    • Author(s)
      Kenichi Yamaguchi et al.
    • Journal Title

      Matrix Biol. 24

      Pages: 283-294

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Multiple proteins are involved in the protein-DNA complex in the proximal promoter of the human alpha1(III) collagen gene (COL3A1).2005

    • Author(s)
      Tomoaki Yoshino et al.
    • Journal Title

      Biochim Biophys Acta. 1729

      Pages: 94-104

    • Related Report
      2005 Annual Research Report
  • [Journal Article] This paper is in Japanese.

    • Journal Title

      This paper is in Japanese.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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