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A New Function of The Lubricin and It's Role in the Pathogenesis of Arthritis.

Research Project

Project/Area Number 17591585
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKagoshima University

Principal Investigator

YAMAMOTO Takuya  Kagoshima University, Medical and Dental Hospital, Research Associate, 医学部・歯学部附属病院, 助手 (40381157)

Co-Investigator(Kenkyū-buntansha) KOMIYA Setsuro  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院医歯学総合研究科, 教授 (30178371)
MATSUYAMA Takami  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院医歯学総合研究科, 教授 (30145479)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsLubricin / Rheumatoid Arthritis / sMSF protein / Hyaluronic acid / 蛋白質 / 免疫学
Research Abstract

We previously found that the lubricin mRNA deleting the exon 6 (sMSF) is present in synovial fluids from rheumatoid arthritis (RA). To detect the sMSF protein, rabbit anti-human lubricin polyclonal antibody was produced immunizing the purified lubricin, and that reactivity with lubricin and sMSF recombinant protein were confirmed. In the detection of sMSF using that antibody and western blotting, a lot of the sMSF protein was present in synovial fluids from RA patients in comparison with that from OA patients.
The presence of the complex of lubricin and hyaluronic acid (HA) has not been clarified in vivo. Therefore, we tried to examine that complex in synovial fluids from RA and OA patients. The molecular weight (M.W.) of lubricin was ranged from 300 kDa to 5,000 kDa in RA and OA synovial fluids in the fractionation using Sepharose-S 500. There was much of the lower M.W. lubricin in synovial fluids from RA patients than OA patients. To determine whether the high M.W. lubricin is the complex with HA, HA was trapped with HA binding protein (HABP) that is specifically bound to HA, and then, lubricin in the HABP binding proteins was detected using anti-lubricin antibody and western blotting. The results showed that lubricin in RA and OA synovial fluids was present in the complex with HA. In addition, we found that lubricin is bound to joint cartilage via HA.
Recently, the high M.W. (3,000 kDa) or low M.W. (1,000 kDa) HA are available as a drug for treatment of arthritis. Thus, it is an interesting subject to know how these HA are contributed to the boundary lubrication by forming the complex with lubricin.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (6 results)

All 2006

All Journal Article (6 results)

  • [Journal Article] Expression of similar MSF variant in RA synovial fluids2006

    • Author(s)
      Tsuneyoshi Y
    • Journal Title

      Modern Rheumatology 16

      Pages: 109-109

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] The Z39Ig protein is a specific marker of synovial A cells in RA synovium2006

    • Author(s)
      Tanaka M
    • Journal Title

      Arthritis and Rheumatism 54・9

      Pages: 638-639

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] In vitro in vivo efficacies of the recombinant immunotoxin against the folate receptor β on RA synovial cells2006

    • Author(s)
      Nagai T
    • Journal Title

      Arthritis and Rheumatism 54・10

      Pages: 3126-3134

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Expression of similar MSF variant in RA synovial fluids2006

    • Author(s)
      Tsuneyoshi Y, Yamamoto T, et al.
    • Journal Title

      Modern Rheumatology 16

      Pages: 109-109

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The Z39Ig protein is a specific marker of synovial A cells in RA synovium2006

    • Author(s)
      Tanaka M, et al.
    • Journal Title

      Arthritis and Rheumatism 54. 9

      Pages: 638-639

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vitro in vivo efficacies of the recombinant immunotoxin against the folate receptor β on RA synovial cells2006

    • Author(s)
      Nagai T, et al.
    • Journal Title

      Arthritis and Rheumatism 54. 10

      Pages: 3126-3134

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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