| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Research Abstract |
We have identified neurotrophic receptors (trkA, trkB, trkC) and neurotrophic factors (NGF, BDNF, NT-3) in 8 cases of Ewing/PNET family tumors by RT-PCR. Consequently, these factors were analyzed by immunohistochemistry, trkA, and trkB were positive in 7/8 and, NGF was positive in 5/8. There were 5 cases co-expressing both trkA and NGF, which suggested autocrine loop mechanism in tumor growth. In this study, there were discrepancy in the expression of these factors between Ewing's sarcoma of bone (ESB) and extraskeletal Ewing's sarcoma (ESK). We performed oligonucleotide microarray analysis for 11 cases of Ewing sarcoma (ESB: 5 cases, ESK: 6 cases) to analyze the tendency of the gdne expression profile. The result of cluster analysis and tree view of 54, 676 genes expression profile had suggested that there was no relationship between gene expression profile and location of tumor. Signal expression pattern of genes of neurotrophic receptors and neurotrophic factors were relatively low, but inverse relationship were observed between them. We found up-regulated gene expressions in IGF1-R, ID2, CD99, and down-regulated in TGFB-R2.
The results of RT-PCR and signal expression patterns by oligonucleotide microarray analysis were well-correlated, but there were discrepancies between the results of RT-PCR and immunohistochemistry. Further studies of gene analysis with more samples are mandatory.
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