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Prognostic factors in Ewing/PNET family tumors

Research Project

Project/Area Number 17591598
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTeikyo University

Principal Investigator

ABE Satoshi  Teikyo University, School of Medicine, Lecturer (30246053)

Co-Investigator(Kenkyū-buntansha) IMAMURA Tetsuo  Teikyo University, School of Medicine, Professor (40010566)
TSUJI Kaori  Teikyo University, School of Medicine, Research Assistant (40198833)
KASHIMA Kenji  Tokyo University Hospital, 医学部付属病院, Research Assistant (70292729)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsEwing / PNET family tumor / Neural differentiation / Immunohistochemistry / Microarray / Prognosis / 融合遺伝子
Research Abstract

We have identified neurotrophic receptors (trkA, trkB, trkC) and neurotrophic factors (NGF, BDNF, NT-3) in 8 cases of Ewing/PNET family tumors by RT-PCR. Consequently, these factors were analyzed by immunohistochemistry, trkA, and trkB were positive in 7/8 and, NGF was positive in 5/8. There were 5 cases co-expressing both trkA and NGF, which suggested autocrine loop mechanism in tumor growth. In this study, there were discrepancy in the expression of these factors between Ewing's sarcoma of bone (ESB) and extraskeletal Ewing's sarcoma (ESK). We performed oligonucleotide microarray analysis for 11 cases of Ewing sarcoma (ESB: 5 cases, ESK: 6 cases) to analyze the tendency of the gdne expression profile. The result of cluster analysis and tree view of 54, 676 genes expression profile had suggested that there was no relationship between gene expression profile and location of tumor. Signal expression pattern of genes of neurotrophic receptors and neurotrophic factors were relatively low, but inverse relationship were observed between them. We found up-regulated gene expressions in IGF1-R, ID2, CD99, and down-regulated in TGFB-R2.
The results of RT-PCR and signal expression patterns by oligonucleotide microarray analysis were well-correlated, but there were discrepancies between the results of RT-PCR and immunohistochemistry. Further studies of gene analysis with more samples are mandatory.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (5 results)

All 2007

All Journal Article (5 results)

  • [Journal Article] 骨および骨外性Ewing肉腫におけるマイクロアレイ法による網羅的遺伝子解析:発生部位による遺伝子的差異の検討2007

    • Author(s)
      三木 勇治
    • Journal Title

      帝京医学雑誌 30(6)

      Pages: 313-320

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Clinical outcome of patients with Ewing sarcoma family of tumors of bone in Japan:the Japanese Musculoskeletal Oncology Group cooperative study2007

    • Author(s)
      Obata H, Ueda T, Kawai A, Ishii T, Ozaki T, Abe S, Tanaka K, Tsuchiya H, Matsumine A, Yabe H
    • Journal Title

      Cancer 109(4)

      Pages: 767-775

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gene expression profile of Ewing sarcoma of bone and extraskeletal Ewing sarcoma : Comparison of genetic differences in their location2007

    • Author(s)
      Miki, Y
    • Journal Title

      Teikyo Medical Journal 30(6)

      Pages: 313-320

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] the Japanese Musculoskeletal Oncology Group. Clinical outcome of patients with Ewing sarcoma family of tumors of bone in Japan : the Japanese Musculoskeletal Oncology Group cooperative study2007

    • Author(s)
      Obata H, Ueda T, Kawai A, Ishii T, Ozaki T, Abe S, Tanaka K, Tsuchiya H, Matsumine A, Yabe H
    • Journal Title

      Cancer 109(4)

      Pages: 767-775

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Clinical outcome of patients with Ewing sarcoma family of tumors of bone in Japan : the Japanese Musculoskeletal Oncology Group cooperative study.2007

    • Author(s)
      Hideto Obata et al.
    • Journal Title

      Cancer 109(4)

      Pages: 767-75

    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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