| Project/Area Number |
17591599
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Teikyo University |
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Principal Investigator |
TAKAI Shinro Teikyo University, Medicine, Professor, 医学部, 教授 (10226730)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Yoshinobu Teikyo University, Medicine, Associate Professor, 医学部, 講師 (00295651)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | articular cartilage / osteochondral defect / tissue regeneration / tidemark / mesenchymal cell / autologous cell transplantation / transgenic rat / in situ hybridization |
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| Research Abstract |
The research which used the autologous cell graft for joint cartilage defect restoration has accomplished mostly. Restoration of normal hyaline cartilage has not been attained. We have completed the method for distinguishing donor and recipient cells using the combination of transgenic rat and DNA in situ hybridization. We reported those in the 48th and 49th Annual Meeting of the Orthopaedic Research Society (ORS, US), it was clearly demonstrated that cells from surrounding bone marrow replace the underlining bone of the donor with the donor cells. The model of following the transplanted cells was completed using GFP transgenic rats. This method is more reliable than the previously reported method using transgenic rat and DNA in situ hybridization. Transplantation of osteochondral autograft is widely used as a therapeutic strategy for the full thickness defect of articular cartilage. Although the viable graft has been demonstrated after the transplantation, the fate of host (intrinsic)
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and donor (extrinsic) cells and their effect on the biomechanical properties have not been elucidated. The objective of this study was to follow intrinsic and extrinsic cells and to examine the biomechanical properties in the healing tissue after transplantation of osteochondral autograft, An autologous transplantation model was used, in which a donor transgenic rat and recipient wild-type rat was genetically identical each other except for transgenes. It was hypothesized that intrinsic and extrinsic cells contribute to the regeneration of osteochondral defect after the transplantation of osteochondral autograft and affect viscoelastic properties of healing autograft. Intrinsic and extrinsic cells were clearly detected up to 12 weeks after transplantation. Extrinsic cells began to be identified close to the newly synthesized bone in the thickened cartilage at 6 weeks, and the regeneration of the subchondral bone completed by 12 weeks. It was also demonstrated that the remodeling of the subchondral bone occurred by enchondral ossification of extrinsic chondrocytes. Viscoelastic properties of the autograft at 6 weeks became inferior compared to those of the control, followed by the recovery up to control level by 12 weeks while the enchondral ossification was found. Less
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