| Project/Area Number |
17591612
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | University of Tsukuba |
|---|
Principal Investigator |
INOMATA Shinichi University of Tsukuba, Graduate School of Comprehensive Human Sciences, Associate Professor (10282352)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIYOSHI Hiroyuki RIKEN Bio Resource Center, Subteam for Manipulation of Cell Fate, Team leader (70219830)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥4,020,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥120,000)
Fiscal Year 2007: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
|
|---|
| Keywords | severe pain / enkephalin / virus vector / gene therapy / ウイルスベクター / ペインクリニック / 疼痛 |
|---|
| Research Abstract |
Total RNA of a human adrenal gland was obtained from patients, received surgical operation, with their informed consents. The cDNA was synthesized by reverse transcriptase using a primer. The pre-pro-enkephalin (PPE) gene was then amplified by polymerase chain reaction with specific upstream and downstream oligoneucleotides. The polymerase chain reaction product contained the full-length PPE coding cDNA. The amplified fragment was cloned into a vector (pDON-AI-2-Neo or pMEI-5 Neo [Not I-BamHI]). The plasmid DNA was purified and digested with restriction enzymes and electrophoresed on agarose gel. The higher efficacy of infection was observed in pDON-AI-2-Neo.
|
