Investigation of the role of the fibrinolytic system in cardiac remodeling and its clinical relevance

• Project/Area Number: 17591623
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Gifu University
• Principal Investigator: AKAMATSU Shigeru Gifu University, graduate School of Medicine, Part time lecturer, 大学院医学系研究科, 非常勤講師 (20167828)
• Co-Investigator(Kenkyū-buntansha): KOZAWA Osamu Gifu University, graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (90225417) ; DOHI Shuji Gifu University, graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (40155627)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,800,000 (Direct Cost: ¥3,800,000); Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
• Keywords: cardiac myocytes / ischemia / heat shock protein / crstallin / molecular chaperone / 血小板 / 血栓 / 凝固・線溶系

Research Abstract

We investigated the role of the fibrinolytic system in cardiac remodeling and its clinical relevance. αB-crystallin is the most abundant low-molecular-weight heat shock protein in heart and recent studies have demonstrated that it plays a cardioprotective role during myocardial infarction both in vivo and in vitro. On the other hand, platelet-derived growth factor (PDGF), a potent serum mitogen, has been reported to improve cardiac function after myocardial infarction. Using a mouse myocardial infarction model, we investigated whether αB-crystallin is phosphorylated during myocardial infarction and the implication of PDGF-BB. Phosphorylation of αB-crystallin at Ser-59 was time dependently induced and plasma PDGF-BB levels were concomitantly increased. Moreover, PDGF-BB-stimulated phosphorylation of αB-crystallin was suppressed by SB203580, a specific inhibitor of p38 mitogen-activated protein kinase, in primary cultured cardiac myocytes.

Research Products

• [Journal Article] Midazolam suppresses thrombin-induced heat shock protein 27 phosphorylation through inhibition of p38 mitogen-activated protein kinase in cardiac myocytes. (2005)
  • Author(s): Tanabe K, Akamatsu S, Suga H, Takai S, Kato K, Dohi S, Kozawa O
  • Journal Title: J. Cell. Biochem. 96 Pages: 56-64
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Possible involvement of phosphatidylinositol 3-kinase/Akt signal pathway in vasopressin-induced HSP27 phosphorylation in aortic smooth muscle A10 cells. (2005)
  • Author(s): Suga H, Nakajima K, Shu E, Kanno Y, Hirade K, Ishisaki A, Matsuno H, Tanabe K, Akamatsu S, Kato K, Oiso Y, Kozawa O
  • Journal Title: Arch. Biochem. Biophys 438 Pages: 137-145
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] αB-crystallin is phosphorylated during myocardial infarction : involvement of platelet-derived growth factor-BB. (2005)
  • Author(s): Shu E, Matsuno H, Akamatsu S, Kanno Y, Suga H, Nakajima K, Ishisaki A, Kato K, Kitajima Y, Kozawa O
  • Journal Title: Arch. Biochem. Biophys. 438 Pages: 111-118
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Possible involvement of phosphatidylinositol 3-kinase/Akt signal pathway in vasopressin-induced HSP27 phosphorylation in aortic smooth muscle A10 cells. (2005)
  • Author(s): Suga H, Nakajima K, Shu E, Kanno Y, Hirade K, Ishisaki A, Matsuno H, Tanabe K, Akamatsu S, Kato K, Oiso Y, Kozawa O
  • Journal Title: Arch. Biochem. Biophys. 438 Pages: 137-145
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] αB-crystallin is phosphorylated during myocardial infarction : involvement of platelet-derived growth factor-BB. (2005)
  • Author(s): Shu E, Matsuno H, Akamatsu S, Kanno Y, Suga H, Nakajima K, Ishisaki A, Kato K, Kitajima Y, Kozawa O
  • Journal Title: Arch.Biochem.Biophys. 438 Pages: 111-118