| Project/Area Number |
17591623
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Gifu University |
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Principal Investigator |
AKAMATSU Shigeru Gifu University, graduate School of Medicine, Part time lecturer, 大学院医学系研究科, 非常勤講師 (20167828)
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| Co-Investigator(Kenkyū-buntansha) |
KOZAWA Osamu Gifu University, graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (90225417)
DOHI Shuji Gifu University, graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (40155627)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | cardiac myocytes / ischemia / heat shock protein / crstallin / molecular chaperone / 血小板 / 血栓 / 凝固・線溶系 |
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| Research Abstract |
We investigated the role of the fibrinolytic system in cardiac remodeling and its clinical relevance. αB-crystallin is the most abundant low-molecular-weight heat shock protein in heart and recent studies have demonstrated that it plays a cardioprotective role during myocardial infarction both in vivo and in vitro. On the other hand, platelet-derived growth factor (PDGF), a potent serum mitogen, has been reported to improve cardiac function after myocardial infarction. Using a mouse myocardial infarction model, we investigated whether αB-crystallin is phosphorylated during myocardial infarction and the implication of PDGF-BB. Phosphorylation of αB-crystallin at Ser-59 was time dependently induced and plasma PDGF-BB levels were concomitantly increased. Moreover, PDGF-BB-stimulated phosphorylation of αB-crystallin was suppressed by SB203580, a specific inhibitor of p38 mitogen-activated protein kinase, in primary cultured cardiac myocytes.
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