The contribution of lung protective ventilation and anti-cytokine therapy in ventilator induced lung injury
| Project/Area Number |
17591628
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka University |
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Principal Investigator |
FUJINO Yuji Osaka University, Graduate School of Medicine, Lecturer, 医学系研究科, 講師 (50252672)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIYAMA Akinori Osaka University, Hospital, Assistant, 医学部附属病院, 助手 (00324856)
OHTA Noriyuki Osaka university, Hospital, Assistant, 医学部附属病院, 助手 (60379162)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Acute lung injury / HFO / mechanical ventilation / proinflammatory cytokine / chemokine receptor / quantitative RT-PCR / 急性呼吸不全 / 肺傷害 / 高頻度換気法 |
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| Research Abstract |
Prior to the assessment of anti-cytokine therapy, we tried to elucidate the effect of pCO2 on the production of proinflammatory cytokine in order to determine the effect of pCO2 in HFO upon experimental results in animal study.
Wistar rats were ventilated and administered HCl intratracheally. After HC1 injection, rats were randomized to two groups: hypercapnia group and normocapnia group. The animals were ventilated with HFO for 4hrs. Lung volume was titrated to achieve PaCO2 40-50 mmHg (normocapnia group) or 80mmHg (hypercapnia group). After 4hrs of mechanical ventilation, the level of TNF-a,IL-6,and CINC-1 in BAL fluid was determined. No significant difference was observed between these two groups. However, W/D ratio and histological study showed that hypercapnia attenuated lung injury.
Furthermore, we tried to establish quantitative RT-PCR system of chemokine receptors(CCR4,5,6,7,9,CXCR3,4) by the use of colitis model. Chemokine receptors except CCR6 were upregulated in diseased mice. Interestingly, mRNA of CCR7 and CXCR4 were upregulated in diseased colon compared with non-diseased small intestine.
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Report
(3 results)
Research Products
(12 results)
