| Project/Area Number |
17591642
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of Miyazaki |
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Principal Investigator |
SHIRASAKA Tetsuro University of Miyazaki, Faculty of Medicine, Lecture, 医学部, 講師 (00274788)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATO Kunitake University of Miyazaki, Faculty of Medicine, Associate Professor, 医学部, 助手 (20234461)
MATSUOKA Hiroshi University of Miyazaki, Faculty of Medicine, Associate Professor, 医学部, 助手 (30315379)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Dexmedetomidine / Orexin / PVN / Potassium channel / α2-agonist / Membrane Current / カリウムチャネル / G1RL / GIRK |
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| Research Abstract |
We investigated interaction between dexmedetomidine (DEX) and orexin (ORX) system. First, we identified ORX neuron by observing depolarizing effect induced by ORX application. Nest, we examined the effect of DEX (10^<-6>M) on depolarization induced by ORX-A (10^<-6>M). We bath-applied ORX-A (10^<-6>M) for one minute and applied DEX (10^<-6>M). Depolarization induced by ORX followed by hyperpolarization induced by DEX was observed. DEX hyperpolarized PVN neuron dose-dependently (3x10^<-9>〜6x 10^<-6>M). The effect of DEX was blocked by an α2-adrenoceptor antagonist, yohimbine (10^<-5>M) and a G-protein-coupled inwardly rectifying K^+ (GIRK) channel blocker, tertiapin.
Next, we examined the effect of SB-334867, an ORX-antagonist on the hyperpolarization induced by DEX. We first bath-applied SB-334867 (10^<-5>M) or artificial cerebrospinal fluid (control) for 5 min and applied DEX (10^<-6> M) for 2 min. We compared SB-334867 group or control group. Significant difference was not observed between two groups. We could not find the interaction between DEX and ORX system at the cell level.
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